Molecular characterization of the gastrointestinal eukaryotic virome in elderly people in Belem, Para, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | http://patua.iec.gov.br//handle/iec/4545 |
Resumo: | Acute gastroenteritis is one of the main causes of mortality and morbidity worldwide, affecting mainly children, the immunocompromised and elderly people. Enteric viruses, especially rotavirus A, are considered important etiological agents, while long-term care facilities are considered favorable environments for the occurrence of sporadic cases and outbreaks of acute gastroenteritis. Therefore, it is important to monitor the viral agents present in nursing homes, especially because studies involving the elderly population in Brazil are scarce, resulting in a lack of available virological data. As a result, the causative agent remains unidentified in a large number of reported acute gastroenteritis cases. However, the advent of next-generation sequencing provides new opportunities for viral detection and discovery. The aim of this study was to identify the viruses that circulate among elderly people with and without acute gastroenteritis, living in residential care homes in Belém, Pará, Brazil, between 2017 and 2019. Ninety-three samples were collected and screened by immunochromatography and qPCR. After, the samples were analyzed individually or in pools by next generation sequencing to identify the viruses circulating in this population. In 26 sequenced samples, members of 13 eukaryotic virus families were identified. The most abundantly present virus families were Parvoviridae, Genomoviridae and Smacoviridae. Contigs displaying similarity to pegiviruses were also detected. Furthermore, a near-complete rotavirus A genome was obtained and could be classified as G3P[8] genotype with the equine DS-1-like genetic background. Complete sequences of the VP4 and VP7 genes of a rotavirus C were also detected, belonging to G4P[2]. This study demonstrates the first characterization of the gastrointestinal virome in elderly in Northern Brazil. A diversity of viruses was found to be present in patients with and without diarrhea, reinforcing the need to monitor elderly people residing in long-term care facilities, especially in cases of acute gastroenteritis. |
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Portal, Thayara MoraisVanmechelen, BertVan Espen, LoreJansen, DaanTeixeira, Dielle MonteiroSousa, Emanuella Sarmento Alho deSilva, Victor Pereira daLima, Juliana Silva deReymão, Tammy Katlhyn AmaralSequeira, Carina GuilhonVentura, Ana Maria Revorêdo da SilvaSilva, Luciana Damascena daResque, Hugo ReisMetthijnssens, JelleGabbay, Yvone Benchimol2022-06-03T13:11:56Z2022-06-03T13:11:56Z2022PORTAL, Thayara Morais et al. Molecular characterization of the gastrointestinal eukaryotic virome in elderly people in Belem, Para, Brazil. Infection, Genetics and Evolution, v. 99, n. 105241, p. 1-10, Apr. 2022.1567-1348http://patua.iec.gov.br//handle/iec/454510.1016/j.meegid.2022.105241Acute gastroenteritis is one of the main causes of mortality and morbidity worldwide, affecting mainly children, the immunocompromised and elderly people. Enteric viruses, especially rotavirus A, are considered important etiological agents, while long-term care facilities are considered favorable environments for the occurrence of sporadic cases and outbreaks of acute gastroenteritis. Therefore, it is important to monitor the viral agents present in nursing homes, especially because studies involving the elderly population in Brazil are scarce, resulting in a lack of available virological data. As a result, the causative agent remains unidentified in a large number of reported acute gastroenteritis cases. However, the advent of next-generation sequencing provides new opportunities for viral detection and discovery. The aim of this study was to identify the viruses that circulate among elderly people with and without acute gastroenteritis, living in residential care homes in Belém, Pará, Brazil, between 2017 and 2019. Ninety-three samples were collected and screened by immunochromatography and qPCR. After, the samples were analyzed individually or in pools by next generation sequencing to identify the viruses circulating in this population. In 26 sequenced samples, members of 13 eukaryotic virus families were identified. The most abundantly present virus families were Parvoviridae, Genomoviridae and Smacoviridae. Contigs displaying similarity to pegiviruses were also detected. Furthermore, a near-complete rotavirus A genome was obtained and could be classified as G3P[8] genotype with the equine DS-1-like genetic background. Complete sequences of the VP4 and VP7 genes of a rotavirus C were also detected, belonging to G4P[2]. This study demonstrates the first characterization of the gastrointestinal virome in elderly in Northern Brazil. A diversity of viruses was found to be present in patients with and without diarrhea, reinforcing the need to monitor elderly people residing in long-term care facilities, especially in cases of acute gastroenteritis.This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brasil (CAPES) – Finance Code 001 / Fonds Wetenschappelijk Onderzoek (FWO) (1S78019N)Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Programa de Pós-Graduação em Virologia. Ananindeua, PA, BrasilKU Leuven-University of Leuven. Rega Institute Department of Microbiology, Immunology and Transplantation. Laboratory of Clinical and Epidemiological Virology. Leuven, BelgiumKU Leuven-University of Leuven. Rega Institute Department of Microbiology, Immunology and Transplantation. Laboratory of Clinical and Epidemiological Virology. Leuven, BelgiumKU Leuven-University of Leuven. Rega Institute Department of Microbiology, Immunology and Transplantation. Laboratory of Clinical and Epidemiological Virology. Leuven, BelgiumMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Programa de Pós-Graduação em Virologia. Ananindeua, PA, BrasilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Programa de Iniciação Científica Cnpq - Fapespa. Ananindeua, PA, BrasilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Programa de Iniciação Científica Cnpq - Fapespa. Ananindeua, PA, BrasilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Programa de Iniciação Científica Cnpq - Fapespa. Ananindeua, PA, BrasilFederal University of Pará. Institute of Biological Sciences. Biology of Infectious and Parasitic Agents Graduate Program,. Belém, PA, BrazilState University of Pará. Belém, PA, BrazilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, BrasilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, BrasilMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, BrasilKU Leuven-University of Leuven. Rega Institute Department of Microbiology, Immunology and Transplantation. Laboratory of Clinical and Epidemiological Virology. Leuven, BelgiumMinistério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. 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dc.title.pt_BR.fl_str_mv |
Molecular characterization of the gastrointestinal eukaryotic virome in elderly people in Belem, Para, Brazil |
title |
Molecular characterization of the gastrointestinal eukaryotic virome in elderly people in Belem, Para, Brazil |
spellingShingle |
Molecular characterization of the gastrointestinal eukaryotic virome in elderly people in Belem, Para, Brazil Portal, Thayara Morais Gastroenterite / virologia Infecções por Rotavirus Habitação para Idosos Instituição de Longa Permanência para Idosos |
title_short |
Molecular characterization of the gastrointestinal eukaryotic virome in elderly people in Belem, Para, Brazil |
title_full |
Molecular characterization of the gastrointestinal eukaryotic virome in elderly people in Belem, Para, Brazil |
title_fullStr |
Molecular characterization of the gastrointestinal eukaryotic virome in elderly people in Belem, Para, Brazil |
title_full_unstemmed |
Molecular characterization of the gastrointestinal eukaryotic virome in elderly people in Belem, Para, Brazil |
title_sort |
Molecular characterization of the gastrointestinal eukaryotic virome in elderly people in Belem, Para, Brazil |
author |
Portal, Thayara Morais |
author_facet |
Portal, Thayara Morais Vanmechelen, Bert Van Espen, Lore Jansen, Daan Teixeira, Dielle Monteiro Sousa, Emanuella Sarmento Alho de Silva, Victor Pereira da Lima, Juliana Silva de Reymão, Tammy Katlhyn Amaral Sequeira, Carina Guilhon Ventura, Ana Maria Revorêdo da Silva Silva, Luciana Damascena da Resque, Hugo Reis Metthijnssens, Jelle Gabbay, Yvone Benchimol |
author_role |
author |
author2 |
Vanmechelen, Bert Van Espen, Lore Jansen, Daan Teixeira, Dielle Monteiro Sousa, Emanuella Sarmento Alho de Silva, Victor Pereira da Lima, Juliana Silva de Reymão, Tammy Katlhyn Amaral Sequeira, Carina Guilhon Ventura, Ana Maria Revorêdo da Silva Silva, Luciana Damascena da Resque, Hugo Reis Metthijnssens, Jelle Gabbay, Yvone Benchimol |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Portal, Thayara Morais Vanmechelen, Bert Van Espen, Lore Jansen, Daan Teixeira, Dielle Monteiro Sousa, Emanuella Sarmento Alho de Silva, Victor Pereira da Lima, Juliana Silva de Reymão, Tammy Katlhyn Amaral Sequeira, Carina Guilhon Ventura, Ana Maria Revorêdo da Silva Silva, Luciana Damascena da Resque, Hugo Reis Metthijnssens, Jelle Gabbay, Yvone Benchimol |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Gastroenterite / virologia Infecções por Rotavirus Habitação para Idosos Instituição de Longa Permanência para Idosos |
topic |
Gastroenterite / virologia Infecções por Rotavirus Habitação para Idosos Instituição de Longa Permanência para Idosos |
description |
Acute gastroenteritis is one of the main causes of mortality and morbidity worldwide, affecting mainly children, the immunocompromised and elderly people. Enteric viruses, especially rotavirus A, are considered important etiological agents, while long-term care facilities are considered favorable environments for the occurrence of sporadic cases and outbreaks of acute gastroenteritis. Therefore, it is important to monitor the viral agents present in nursing homes, especially because studies involving the elderly population in Brazil are scarce, resulting in a lack of available virological data. As a result, the causative agent remains unidentified in a large number of reported acute gastroenteritis cases. However, the advent of next-generation sequencing provides new opportunities for viral detection and discovery. The aim of this study was to identify the viruses that circulate among elderly people with and without acute gastroenteritis, living in residential care homes in Belém, Pará, Brazil, between 2017 and 2019. Ninety-three samples were collected and screened by immunochromatography and qPCR. After, the samples were analyzed individually or in pools by next generation sequencing to identify the viruses circulating in this population. In 26 sequenced samples, members of 13 eukaryotic virus families were identified. The most abundantly present virus families were Parvoviridae, Genomoviridae and Smacoviridae. Contigs displaying similarity to pegiviruses were also detected. Furthermore, a near-complete rotavirus A genome was obtained and could be classified as G3P[8] genotype with the equine DS-1-like genetic background. Complete sequences of the VP4 and VP7 genes of a rotavirus C were also detected, belonging to G4P[2]. This study demonstrates the first characterization of the gastrointestinal virome in elderly in Northern Brazil. A diversity of viruses was found to be present in patients with and without diarrhea, reinforcing the need to monitor elderly people residing in long-term care facilities, especially in cases of acute gastroenteritis. |
publishDate |
2022 |
dc.date.accessioned.fl_str_mv |
2022-06-03T13:11:56Z |
dc.date.available.fl_str_mv |
2022-06-03T13:11:56Z |
dc.date.issued.fl_str_mv |
2022 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PORTAL, Thayara Morais et al. Molecular characterization of the gastrointestinal eukaryotic virome in elderly people in Belem, Para, Brazil. Infection, Genetics and Evolution, v. 99, n. 105241, p. 1-10, Apr. 2022. |
dc.identifier.uri.fl_str_mv |
http://patua.iec.gov.br//handle/iec/4545 |
dc.identifier.issn.-.fl_str_mv |
1567-1348 |
dc.identifier.doi.-.fl_str_mv |
10.1016/j.meegid.2022.105241 |
identifier_str_mv |
PORTAL, Thayara Morais et al. Molecular characterization of the gastrointestinal eukaryotic virome in elderly people in Belem, Para, Brazil. Infection, Genetics and Evolution, v. 99, n. 105241, p. 1-10, Apr. 2022. 1567-1348 10.1016/j.meegid.2022.105241 |
url |
http://patua.iec.gov.br//handle/iec/4545 |
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eng |
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eng |
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Elsevier |
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Elsevier |
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