The IL6-174G/C polymorphism associated with high levels of IL-6 contributes to HCV infection, but Is not related to HBV infection, in the Amazon Region of Brazil
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Digital do Instituto Evandro Chagas (Patuá) |
Texto Completo: | https://patua.iec.gov.br/handle/iec/4531 |
Resumo: | The dysregulation of cytokine production can lead to an inefficient immune response, promoting viral persistence that induces the progression of chronic viral hepatitis. The study investigated the association of the IL6-174G/C polymorphism with changes in cytokine levels and its influence on the persistence and progression of chronic hepatitis caused by HBV and HCV in 72 patients with chronic hepatitis B (HBV), 100 patients with hepatitis C (HCV), and a control group of 300 individuals. The genotyping of the IL6-174G/C polymorphism was performed by real-time PCR, and cytokine levels were measured by enzyme-linked immunosorbent assay (ELISA). HCV patients with the wild-type genotype (GG) had a higher viral load (p = 0.0230). The plasma levels of IL-6 were higher among patients infected with HBV and HCV than among the control group (p < 0.0001). Patients with HCV were associated with increased inflammatory activity (A2-A3; p < 0.0001). In hepatitis C, carriers of the GG genotype had higher levels of IL-6 (p = 0.0286), which were associated with A2-A3 inflammatory activity (p = 0.0097). Patients with A2-A3 inflammatory activity and GG genotype had higher levels of IL-6 than those with the GC/CC genotype (p = 0.0127). In conclusion, the wild-type genotype for the IL6-174G/C polymorphism was associated with high levels of IL-6 and HCV viral load and inflammatory activity, suggesting that this genotype may be a contributing factor to virus-induced chronic infection. |
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Queiroz, Maria Alice FreitasSantiago, Angélica MenezesMoura, Tuane Carolina FerreiraAmoras, Ednelza da Silva GraçaConde, Simone Regina Souza da SilvaVallinoto, Izaura Maria Vieira CayresIshak, RicardoVallinoto, Antonio Carlos Rosário2022-06-01T14:44:04Z2022-06-01T14:44:04Z2022QUEIROZ, Maria Alice Freitas et al. The IL6-174G/C polymorphism associated with high levels of IL-6 contributes to HCV infection, but Is not related to HBV infection, in the Amazon Region of Brazil. Viruses, v. 14, n. 3, p. 1-13, Feb. 2022.1999-4915https://patua.iec.gov.br/handle/iec/453110.3390/v14030507The dysregulation of cytokine production can lead to an inefficient immune response, promoting viral persistence that induces the progression of chronic viral hepatitis. The study investigated the association of the IL6-174G/C polymorphism with changes in cytokine levels and its influence on the persistence and progression of chronic hepatitis caused by HBV and HCV in 72 patients with chronic hepatitis B (HBV), 100 patients with hepatitis C (HCV), and a control group of 300 individuals. The genotyping of the IL6-174G/C polymorphism was performed by real-time PCR, and cytokine levels were measured by enzyme-linked immunosorbent assay (ELISA). HCV patients with the wild-type genotype (GG) had a higher viral load (p = 0.0230). The plasma levels of IL-6 were higher among patients infected with HBV and HCV than among the control group (p < 0.0001). Patients with HCV were associated with increased inflammatory activity (A2-A3; p < 0.0001). In hepatitis C, carriers of the GG genotype had higher levels of IL-6 (p = 0.0286), which were associated with A2-A3 inflammatory activity (p = 0.0097). Patients with A2-A3 inflammatory activity and GG genotype had higher levels of IL-6 than those with the GC/CC genotype (p = 0.0127). In conclusion, the wild-type genotype for the IL6-174G/C polymorphism was associated with high levels of IL-6 and HCV viral load and inflammatory activity, suggesting that this genotype may be a contributing factor to virus-induced chronic infection.Conselho Nacional de Desenvolvimento Científico e Tecnológico—CNPQ (grants #480128/2013-8, #312979/2018-5, #301869/2017-0) and by the Federal University of Para (PROPESP/PAPQ/2021).Federal University of Pará. Institute of Biological Sciences. Laboratory of Virology. Belém, PA, BrazilFederal University of Pará. Institute of Biological Sciences. Laboratory of Virology. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Programa de Pós-Graduação em Virologia. Ananindeua, PA, BrasilFederal University of Pará. Institute of Biological Sciences. Laboratory of Virology. Belém, PA, Brazil / Federal University of Pará. Institute of Biological Sciences. Graduate Program in Biology of Infectious and Parasitic Agents. Belém, PA, BrazilFederal University of Pará. Institute of Biological Sciences. Laboratory of Virology. Belém, PA, BrazilFederal University of Pará. João de Barros Barreto University Hospital. Pathology Service. Belém, PA, Brazil / Federal University of Pará. Institute of Health Sciences. School of Medicine. Belém, PA, BrazilFederal University of Pará. Institute of Biological Sciences. Laboratory of Virology. Belém, PA, BrazilFederal University of Pará. Institute of Biological Sciences. Laboratory of Virology. Belém, PA, BrazilFederal University of Pará. Institute of Biological Sciences. Laboratory of Virology. Belém, PA, BrazilengMDPIThe IL6-174G/C polymorphism associated with high levels of IL-6 contributes to HCV infection, but Is not related to HBV infection, in the Amazon Region of Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleHepatite Crônica / patologiaVírus da Hepatite A / patogenicidadeVírus de HepatitePolimorfismo Genético / genéticainfo:eu-repo/semantics/openAccessreponame:Repositório Digital do Instituto Evandro Chagas (Patuá)instname:Instituto Evandro Chagas (IEC)instacron:IECORIGINALThe IL6-174G/C polymorphism associated with high levels of IL-6 contributes to HCV infection, but Is not related to HBV infection, in the Amazon Region of Brazil.pdfThe IL6-174G/C polymorphism associated with high levels of IL-6 contributes to HCV infection, but Is not related to HBV infection, in the Amazon Region of Brazil.pdfapplication/pdf1872359https://patua.iec.gov.br/bitstreams/1baca805-9b23-4f14-bdc1-3549657a49fe/download0212e4366f1525396a3e5807ff7d2229MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82182https://patua.iec.gov.br/bitstreams/3840dc57-4084-429e-8629-9422efb3b344/download11832eea31b16df8613079d742d61793MD52TEXTThe IL6-174G/C polymorphism associated with high levels of IL-6 contributes to HCV infection, but Is not related to HBV infection, in the Amazon Region of Brazil.pdf.txtThe IL6-174G/C polymorphism associated with high levels of IL-6 contributes to HCV infection, but Is not related to HBV infection, in the Amazon Region of Brazil.pdf.txtExtracted texttext/plain53229https://patua.iec.gov.br/bitstreams/d51ee4c3-3cd3-4016-a0e8-6d1eefeb99df/download4d4cc6486c6859d32de132661c3cb1e5MD55THUMBNAILThe IL6-174G/C polymorphism associated with high levels of IL-6 contributes to HCV infection, but Is not related to HBV infection, in the Amazon Region of Brazil.pdf.jpgThe IL6-174G/C polymorphism associated with high levels of IL-6 contributes to HCV infection, but Is not related to HBV infection, in the Amazon Region of Brazil.pdf.jpgGenerated Thumbnailimage/jpeg5839https://patua.iec.gov.br/bitstreams/9a03e5a9-28ec-4a3b-a868-5ad00299efe1/download337bd640781737bc98eb2a698d12458bMD56iec/45312022-10-20 23:57:38.192oai:patua.iec.gov.br:iec/4531https://patua.iec.gov.brRepositório InstitucionalPUBhttps://patua.iec.gov.br/oai/requestclariceneta@iec.gov.br || Biblioteca@iec.gov.bropendoar:2022-10-20T23:57:38Repositório Digital do Instituto Evandro Chagas (Patuá) - Instituto Evandro Chagas (IEC)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 |
dc.title.pt_BR.fl_str_mv |
The IL6-174G/C polymorphism associated with high levels of IL-6 contributes to HCV infection, but Is not related to HBV infection, in the Amazon Region of Brazil |
title |
The IL6-174G/C polymorphism associated with high levels of IL-6 contributes to HCV infection, but Is not related to HBV infection, in the Amazon Region of Brazil |
spellingShingle |
The IL6-174G/C polymorphism associated with high levels of IL-6 contributes to HCV infection, but Is not related to HBV infection, in the Amazon Region of Brazil Queiroz, Maria Alice Freitas Hepatite Crônica / patologia Vírus da Hepatite A / patogenicidade Vírus de Hepatite Polimorfismo Genético / genética |
title_short |
The IL6-174G/C polymorphism associated with high levels of IL-6 contributes to HCV infection, but Is not related to HBV infection, in the Amazon Region of Brazil |
title_full |
The IL6-174G/C polymorphism associated with high levels of IL-6 contributes to HCV infection, but Is not related to HBV infection, in the Amazon Region of Brazil |
title_fullStr |
The IL6-174G/C polymorphism associated with high levels of IL-6 contributes to HCV infection, but Is not related to HBV infection, in the Amazon Region of Brazil |
title_full_unstemmed |
The IL6-174G/C polymorphism associated with high levels of IL-6 contributes to HCV infection, but Is not related to HBV infection, in the Amazon Region of Brazil |
title_sort |
The IL6-174G/C polymorphism associated with high levels of IL-6 contributes to HCV infection, but Is not related to HBV infection, in the Amazon Region of Brazil |
author |
Queiroz, Maria Alice Freitas |
author_facet |
Queiroz, Maria Alice Freitas Santiago, Angélica Menezes Moura, Tuane Carolina Ferreira Amoras, Ednelza da Silva Graça Conde, Simone Regina Souza da Silva Vallinoto, Izaura Maria Vieira Cayres Ishak, Ricardo Vallinoto, Antonio Carlos Rosário |
author_role |
author |
author2 |
Santiago, Angélica Menezes Moura, Tuane Carolina Ferreira Amoras, Ednelza da Silva Graça Conde, Simone Regina Souza da Silva Vallinoto, Izaura Maria Vieira Cayres Ishak, Ricardo Vallinoto, Antonio Carlos Rosário |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Queiroz, Maria Alice Freitas Santiago, Angélica Menezes Moura, Tuane Carolina Ferreira Amoras, Ednelza da Silva Graça Conde, Simone Regina Souza da Silva Vallinoto, Izaura Maria Vieira Cayres Ishak, Ricardo Vallinoto, Antonio Carlos Rosário |
dc.subject.decsPrimary.pt_BR.fl_str_mv |
Hepatite Crônica / patologia Vírus da Hepatite A / patogenicidade Vírus de Hepatite Polimorfismo Genético / genética |
topic |
Hepatite Crônica / patologia Vírus da Hepatite A / patogenicidade Vírus de Hepatite Polimorfismo Genético / genética |
description |
The dysregulation of cytokine production can lead to an inefficient immune response, promoting viral persistence that induces the progression of chronic viral hepatitis. The study investigated the association of the IL6-174G/C polymorphism with changes in cytokine levels and its influence on the persistence and progression of chronic hepatitis caused by HBV and HCV in 72 patients with chronic hepatitis B (HBV), 100 patients with hepatitis C (HCV), and a control group of 300 individuals. The genotyping of the IL6-174G/C polymorphism was performed by real-time PCR, and cytokine levels were measured by enzyme-linked immunosorbent assay (ELISA). HCV patients with the wild-type genotype (GG) had a higher viral load (p = 0.0230). The plasma levels of IL-6 were higher among patients infected with HBV and HCV than among the control group (p < 0.0001). Patients with HCV were associated with increased inflammatory activity (A2-A3; p < 0.0001). In hepatitis C, carriers of the GG genotype had higher levels of IL-6 (p = 0.0286), which were associated with A2-A3 inflammatory activity (p = 0.0097). Patients with A2-A3 inflammatory activity and GG genotype had higher levels of IL-6 than those with the GC/CC genotype (p = 0.0127). In conclusion, the wild-type genotype for the IL6-174G/C polymorphism was associated with high levels of IL-6 and HCV viral load and inflammatory activity, suggesting that this genotype may be a contributing factor to virus-induced chronic infection. |
publishDate |
2022 |
dc.date.accessioned.fl_str_mv |
2022-06-01T14:44:04Z |
dc.date.available.fl_str_mv |
2022-06-01T14:44:04Z |
dc.date.issued.fl_str_mv |
2022 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
QUEIROZ, Maria Alice Freitas et al. The IL6-174G/C polymorphism associated with high levels of IL-6 contributes to HCV infection, but Is not related to HBV infection, in the Amazon Region of Brazil. Viruses, v. 14, n. 3, p. 1-13, Feb. 2022. |
dc.identifier.uri.fl_str_mv |
https://patua.iec.gov.br/handle/iec/4531 |
dc.identifier.issn.-.fl_str_mv |
1999-4915 |
dc.identifier.doi.-.fl_str_mv |
10.3390/v14030507 |
identifier_str_mv |
QUEIROZ, Maria Alice Freitas et al. The IL6-174G/C polymorphism associated with high levels of IL-6 contributes to HCV infection, but Is not related to HBV infection, in the Amazon Region of Brazil. Viruses, v. 14, n. 3, p. 1-13, Feb. 2022. 1999-4915 10.3390/v14030507 |
url |
https://patua.iec.gov.br/handle/iec/4531 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
MDPI |
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MDPI |
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Repositório Digital do Instituto Evandro Chagas (Patuá) |
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