Histological processing techniques for the study of Dugesiidae development (Platyhelminthes, Tricladida, Paludicola)

Detalhes bibliográficos
Autor(a) principal: VARA,D. C. da
Data de Publicação: 2001
Outros Autores: LEAL-ZANCHET,A. M., LIZARDO-DAUDT,H. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Biologia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-71082001000200019
Resumo: The objective of the present study was to adapt techniques for the histological processing of Dugesiidae cocoons for the study of embryo development. The cocoons were fixed with formalin, SUSA, Bouin or paraformaldehyde/glutaraldehyde and subsequently embedded in Paraplast or glycol methacrylate (Historesin). Paraplast embedding yielded reasonable results only after the cocoon was perforated or fixed for a prolonged period of time using softening techniques with acid solutions. When the SUSA or Bouin fixative and Historesin embedding techniques were used the results were good for light microscopical analysis. Fixation with paraformaldehyde/glutaraldehyde and glycol methacrylate embedding resulted in better tissue preservation, and did not require prolonged fixation or softening techniques. Thus, we suggest this technique for light microscopical analysis of embryo development in Dugesiidae.
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spelling Histological processing techniques for the study of Dugesiidae development (Platyhelminthes, Tricladida, Paludicola)embryo developmenttechniquesglycol methacrilateTricladidaThe objective of the present study was to adapt techniques for the histological processing of Dugesiidae cocoons for the study of embryo development. The cocoons were fixed with formalin, SUSA, Bouin or paraformaldehyde/glutaraldehyde and subsequently embedded in Paraplast or glycol methacrylate (Historesin). Paraplast embedding yielded reasonable results only after the cocoon was perforated or fixed for a prolonged period of time using softening techniques with acid solutions. When the SUSA or Bouin fixative and Historesin embedding techniques were used the results were good for light microscopical analysis. Fixation with paraformaldehyde/glutaraldehyde and glycol methacrylate embedding resulted in better tissue preservation, and did not require prolonged fixation or softening techniques. Thus, we suggest this technique for light microscopical analysis of embryo development in Dugesiidae.Instituto Internacional de Ecologia2001-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-71082001000200019Revista Brasileira de Biologia v.61 n.2 2001reponame:Revista Brasileira de Biologia (Online)instname:Instituto Internacional de Ecologia (IIE)instacron:IIE10.1590/S0034-71082001000200019info:eu-repo/semantics/openAccessVARA,D. C. daLEAL-ZANCHET,A. M.LIZARDO-DAUDT,H. M.eng2001-08-16T00:00:00Zoai:scielo:S0034-71082001000200019Revistawww.scielo.br/rbbioONGhttps://old.scielo.br/oai/scielo-oai.php||bjb.iie@terra.com.br1806-96060034-7108opendoar:2001-08-16T00:00Revista Brasileira de Biologia (Online) - Instituto Internacional de Ecologia (IIE)false
dc.title.none.fl_str_mv Histological processing techniques for the study of Dugesiidae development (Platyhelminthes, Tricladida, Paludicola)
title Histological processing techniques for the study of Dugesiidae development (Platyhelminthes, Tricladida, Paludicola)
spellingShingle Histological processing techniques for the study of Dugesiidae development (Platyhelminthes, Tricladida, Paludicola)
VARA,D. C. da
embryo development
techniques
glycol methacrilate
Tricladida
title_short Histological processing techniques for the study of Dugesiidae development (Platyhelminthes, Tricladida, Paludicola)
title_full Histological processing techniques for the study of Dugesiidae development (Platyhelminthes, Tricladida, Paludicola)
title_fullStr Histological processing techniques for the study of Dugesiidae development (Platyhelminthes, Tricladida, Paludicola)
title_full_unstemmed Histological processing techniques for the study of Dugesiidae development (Platyhelminthes, Tricladida, Paludicola)
title_sort Histological processing techniques for the study of Dugesiidae development (Platyhelminthes, Tricladida, Paludicola)
author VARA,D. C. da
author_facet VARA,D. C. da
LEAL-ZANCHET,A. M.
LIZARDO-DAUDT,H. M.
author_role author
author2 LEAL-ZANCHET,A. M.
LIZARDO-DAUDT,H. M.
author2_role author
author
dc.contributor.author.fl_str_mv VARA,D. C. da
LEAL-ZANCHET,A. M.
LIZARDO-DAUDT,H. M.
dc.subject.por.fl_str_mv embryo development
techniques
glycol methacrilate
Tricladida
topic embryo development
techniques
glycol methacrilate
Tricladida
description The objective of the present study was to adapt techniques for the histological processing of Dugesiidae cocoons for the study of embryo development. The cocoons were fixed with formalin, SUSA, Bouin or paraformaldehyde/glutaraldehyde and subsequently embedded in Paraplast or glycol methacrylate (Historesin). Paraplast embedding yielded reasonable results only after the cocoon was perforated or fixed for a prolonged period of time using softening techniques with acid solutions. When the SUSA or Bouin fixative and Historesin embedding techniques were used the results were good for light microscopical analysis. Fixation with paraformaldehyde/glutaraldehyde and glycol methacrylate embedding resulted in better tissue preservation, and did not require prolonged fixation or softening techniques. Thus, we suggest this technique for light microscopical analysis of embryo development in Dugesiidae.
publishDate 2001
dc.date.none.fl_str_mv 2001-05-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-71082001000200019
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-71082001000200019
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0034-71082001000200019
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Internacional de Ecologia
publisher.none.fl_str_mv Instituto Internacional de Ecologia
dc.source.none.fl_str_mv Revista Brasileira de Biologia v.61 n.2 2001
reponame:Revista Brasileira de Biologia (Online)
instname:Instituto Internacional de Ecologia (IIE)
instacron:IIE
instname_str Instituto Internacional de Ecologia (IIE)
instacron_str IIE
institution IIE
reponame_str Revista Brasileira de Biologia (Online)
collection Revista Brasileira de Biologia (Online)
repository.name.fl_str_mv Revista Brasileira de Biologia (Online) - Instituto Internacional de Ecologia (IIE)
repository.mail.fl_str_mv ||bjb.iie@terra.com.br
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