Water soluble palmitic acid in media for cultivation of leprosy derived psychrophilic mycobacteria from mycobacterium leprae infected tissues

Detalhes bibliográficos
Autor(a) principal: Kato, Laszlo
Data de Publicação: 1994
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Hansenologia Internationalis (Online)
Texto Completo: https://periodicos.saude.sp.gov.br/hansenologia/article/view/35459
Resumo: Cultivation trials for Mycobacterium leprae resulted in growth of Mycobacterium psychrophilum (L). Media were inoculated with host grown Mycobacterium leprae cells from armadillo tissues, Nu mice foot pads or human lepromata. Cultures were obtained in liquid and on semisolid multifactoria I media containing water soluble palmitic acid or its salts. Ammonium thioglycolate and Na palmitate served as carbon and energy sources. The watersoluble palmitic acid remained in perfect solution following sterilization in the autoclave, thus easily accessible to the cells. The cyclodextrin-Fe complex served asa siderophore to grow the obtained leprosy derived psychrophilic cells. The leprosy derived cultures and subcultures grew optimally at 4-10°C, but deteriorated rapidly at +32°C in the multifactorial media. No growth occurred in 7H9 media. Cultures were not identified for classification. Note: Water soluble palmitic acid culture media are available from this author free of charge for qualified investigators.
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spelling Water soluble palmitic acid in media for cultivation of leprosy derived psychrophilic mycobacteria from mycobacterium leprae infected tissuesBacteriology of leprosyMycobacterium lepraepsychrophllaculturemediapalmitic acidpalmitatesMitsuda reactionCultivation trials for Mycobacterium leprae resulted in growth of Mycobacterium psychrophilum (L). Media were inoculated with host grown Mycobacterium leprae cells from armadillo tissues, Nu mice foot pads or human lepromata. Cultures were obtained in liquid and on semisolid multifactoria I media containing water soluble palmitic acid or its salts. Ammonium thioglycolate and Na palmitate served as carbon and energy sources. The watersoluble palmitic acid remained in perfect solution following sterilization in the autoclave, thus easily accessible to the cells. The cyclodextrin-Fe complex served asa siderophore to grow the obtained leprosy derived psychrophilic cells. The leprosy derived cultures and subcultures grew optimally at 4-10°C, but deteriorated rapidly at +32°C in the multifactorial media. No growth occurred in 7H9 media. Cultures were not identified for classification. Note: Water soluble palmitic acid culture media are available from this author free of charge for qualified investigators.Instituto Lauro de Souza Lima da Secretaria de Estado da Saúde de São Paulo1994-06-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado pelos paresapplication/pdfhttps://periodicos.saude.sp.gov.br/hansenologia/article/view/3545910.47878/hi.1994.v19.35459Hansenologia Internationalis: leprosy and other infectious diseases; Vol. 19 No. 1 (1994); 17-27Hansenologia Internationalis: hanseníase e outras doenças infecciosas; v. 19 n. 1 (1994); 17-271982-5161reponame:Hansenologia Internationalis (Online)instname:Instituto Lauro de Souza Lima (ILSL)instacron:ILSLenghttps://periodicos.saude.sp.gov.br/hansenologia/article/view/35459/33882https://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessKato, Laszlo2023-10-10T16:09:46Zoai:ojs.periodicos.saude.sp.gov.br:article/35459Revistahttps://periodicos.saude.sp.gov.br/hansenologiaPRIhttps://periodicos.saude.sp.gov.br/hansenologia/oaihansen_int@ilsl.br || hansenologia.internationalis@gmail.com || periodicossp@saude.sp.gov.br1982-51610100-3283opendoar:2023-10-10T16:09:46Hansenologia Internationalis (Online) - Instituto Lauro de Souza Lima (ILSL)false
dc.title.none.fl_str_mv Water soluble palmitic acid in media for cultivation of leprosy derived psychrophilic mycobacteria from mycobacterium leprae infected tissues
title Water soluble palmitic acid in media for cultivation of leprosy derived psychrophilic mycobacteria from mycobacterium leprae infected tissues
spellingShingle Water soluble palmitic acid in media for cultivation of leprosy derived psychrophilic mycobacteria from mycobacterium leprae infected tissues
Kato, Laszlo
Bacteriology of leprosy
Mycobacterium leprae
psychrophlla
culturemedia
palmitic acid
palmitates
Mitsuda reaction
title_short Water soluble palmitic acid in media for cultivation of leprosy derived psychrophilic mycobacteria from mycobacterium leprae infected tissues
title_full Water soluble palmitic acid in media for cultivation of leprosy derived psychrophilic mycobacteria from mycobacterium leprae infected tissues
title_fullStr Water soluble palmitic acid in media for cultivation of leprosy derived psychrophilic mycobacteria from mycobacterium leprae infected tissues
title_full_unstemmed Water soluble palmitic acid in media for cultivation of leprosy derived psychrophilic mycobacteria from mycobacterium leprae infected tissues
title_sort Water soluble palmitic acid in media for cultivation of leprosy derived psychrophilic mycobacteria from mycobacterium leprae infected tissues
author Kato, Laszlo
author_facet Kato, Laszlo
author_role author
dc.contributor.author.fl_str_mv Kato, Laszlo
dc.subject.por.fl_str_mv Bacteriology of leprosy
Mycobacterium leprae
psychrophlla
culturemedia
palmitic acid
palmitates
Mitsuda reaction
topic Bacteriology of leprosy
Mycobacterium leprae
psychrophlla
culturemedia
palmitic acid
palmitates
Mitsuda reaction
description Cultivation trials for Mycobacterium leprae resulted in growth of Mycobacterium psychrophilum (L). Media were inoculated with host grown Mycobacterium leprae cells from armadillo tissues, Nu mice foot pads or human lepromata. Cultures were obtained in liquid and on semisolid multifactoria I media containing water soluble palmitic acid or its salts. Ammonium thioglycolate and Na palmitate served as carbon and energy sources. The watersoluble palmitic acid remained in perfect solution following sterilization in the autoclave, thus easily accessible to the cells. The cyclodextrin-Fe complex served asa siderophore to grow the obtained leprosy derived psychrophilic cells. The leprosy derived cultures and subcultures grew optimally at 4-10°C, but deteriorated rapidly at +32°C in the multifactorial media. No growth occurred in 7H9 media. Cultures were not identified for classification. Note: Water soluble palmitic acid culture media are available from this author free of charge for qualified investigators.
publishDate 1994
dc.date.none.fl_str_mv 1994-06-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Avaliado pelos pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.saude.sp.gov.br/hansenologia/article/view/35459
10.47878/hi.1994.v19.35459
url https://periodicos.saude.sp.gov.br/hansenologia/article/view/35459
identifier_str_mv 10.47878/hi.1994.v19.35459
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://periodicos.saude.sp.gov.br/hansenologia/article/view/35459/33882
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Instituto Lauro de Souza Lima da Secretaria de Estado da Saúde de São Paulo
publisher.none.fl_str_mv Instituto Lauro de Souza Lima da Secretaria de Estado da Saúde de São Paulo
dc.source.none.fl_str_mv Hansenologia Internationalis: leprosy and other infectious diseases; Vol. 19 No. 1 (1994); 17-27
Hansenologia Internationalis: hanseníase e outras doenças infecciosas; v. 19 n. 1 (1994); 17-27
1982-5161
reponame:Hansenologia Internationalis (Online)
instname:Instituto Lauro de Souza Lima (ILSL)
instacron:ILSL
instname_str Instituto Lauro de Souza Lima (ILSL)
instacron_str ILSL
institution ILSL
reponame_str Hansenologia Internationalis (Online)
collection Hansenologia Internationalis (Online)
repository.name.fl_str_mv Hansenologia Internationalis (Online) - Instituto Lauro de Souza Lima (ILSL)
repository.mail.fl_str_mv hansen_int@ilsl.br || hansenologia.internationalis@gmail.com || periodicossp@saude.sp.gov.br
_version_ 1796797579676090368

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