Molecular typing of dengue virus type 2 in Brazil

Detalhes bibliográficos
Autor(a) principal: Miagostovich, Marize P.
Data de Publicação: 2003
Outros Autores: Sequeira, Patrícia C., Santos, Flávia B. dos, Maia, Amanda, Nogueira, Rita Maria R., Schatzmayr, Hermann G., Harris, Eva, Riley, Lee W.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/30664
Resumo: Strain typing is a critical tool for molecular epidemiological analysis and can provide important information about the spread of dengue viruses. Here, we performed a molecular characterization of DEN-2 viruses isolated in Brazil during 1990-2000 from geographically and temporally distinct areas in order to investigate the genetic distribution of this serotype circulating in the country. Restriction site-specific polymerase chain reaction (RSS)-PCR presented the same pattern for all 52 Brazilian samples, showing the circulation of just one DEN-2 variant. Phylogenetic analysis using progressive pairwise alignments from 240-nucleotide sequences of the E/NS1 junction in 15 isolates showed that they belong to genotype III (Jamaica genotype).
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spelling Molecular typing of dengue virus type 2 in Brazil Tipificação molecular do vírus da dengue tipo 2 no Brasil Dengue virus type 2Molecular epidemiologyBrazil Strain typing is a critical tool for molecular epidemiological analysis and can provide important information about the spread of dengue viruses. Here, we performed a molecular characterization of DEN-2 viruses isolated in Brazil during 1990-2000 from geographically and temporally distinct areas in order to investigate the genetic distribution of this serotype circulating in the country. Restriction site-specific polymerase chain reaction (RSS)-PCR presented the same pattern for all 52 Brazilian samples, showing the circulation of just one DEN-2 variant. Phylogenetic analysis using progressive pairwise alignments from 240-nucleotide sequences of the E/NS1 junction in 15 isolates showed that they belong to genotype III (Jamaica genotype). A tipagem de cepas é uma ferramenta fundamental para a análise epidemiológica molecular e pode fornecer informações importantes sobre a dispersão dos vírus dengue. Neste trabalho, foi realizada a caracterização molecular de amostras de vírus DEN-2 isoladas no Brasil entre 1990-2000, de áreas geograficamente e temporalmente distintas, com o objetivo de investigar a distribuição genética deste sorotipo circulante no país. A reação em cadeia pela polimerase baseada em sítios de restrição específicos (RSS-PCR) apresentou o mesmo padrão para as 52 amostras Brasileiras, mostrando a circulação de apenas uma variante de vírus DEN-2. A análise filogenética utilizando alinhamento progressivo de sequências de 240 nucleotídeos da junção E/NS1 de 15 cepas mostrou que estas pertencem ao genotipo III (genotipo Jamaica). Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2003-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/30664Revista do Instituto de Medicina Tropical de São Paulo; Vol. 45 No. 1 (2003); 17-21 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 45 Núm. 1 (2003); 17-21 Revista do Instituto de Medicina Tropical de São Paulo; v. 45 n. 1 (2003); 17-21 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/30664/32548Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessMiagostovich, Marize P.Sequeira, Patrícia C.Santos, Flávia B. dosMaia, AmandaNogueira, Rita Maria R.Schatzmayr, Hermann G.Harris, EvaRiley, Lee W.2012-07-07T18:00:09Zoai:revistas.usp.br:article/30664Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:26.172503Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Molecular typing of dengue virus type 2 in Brazil
Tipificação molecular do vírus da dengue tipo 2 no Brasil
title Molecular typing of dengue virus type 2 in Brazil
spellingShingle Molecular typing of dengue virus type 2 in Brazil
Miagostovich, Marize P.
Dengue virus type 2
Molecular epidemiology
Brazil
title_short Molecular typing of dengue virus type 2 in Brazil
title_full Molecular typing of dengue virus type 2 in Brazil
title_fullStr Molecular typing of dengue virus type 2 in Brazil
title_full_unstemmed Molecular typing of dengue virus type 2 in Brazil
title_sort Molecular typing of dengue virus type 2 in Brazil
author Miagostovich, Marize P.
author_facet Miagostovich, Marize P.
Sequeira, Patrícia C.
Santos, Flávia B. dos
Maia, Amanda
Nogueira, Rita Maria R.
Schatzmayr, Hermann G.
Harris, Eva
Riley, Lee W.
author_role author
author2 Sequeira, Patrícia C.
Santos, Flávia B. dos
Maia, Amanda
Nogueira, Rita Maria R.
Schatzmayr, Hermann G.
Harris, Eva
Riley, Lee W.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Miagostovich, Marize P.
Sequeira, Patrícia C.
Santos, Flávia B. dos
Maia, Amanda
Nogueira, Rita Maria R.
Schatzmayr, Hermann G.
Harris, Eva
Riley, Lee W.
dc.subject.por.fl_str_mv Dengue virus type 2
Molecular epidemiology
Brazil
topic Dengue virus type 2
Molecular epidemiology
Brazil
description Strain typing is a critical tool for molecular epidemiological analysis and can provide important information about the spread of dengue viruses. Here, we performed a molecular characterization of DEN-2 viruses isolated in Brazil during 1990-2000 from geographically and temporally distinct areas in order to investigate the genetic distribution of this serotype circulating in the country. Restriction site-specific polymerase chain reaction (RSS)-PCR presented the same pattern for all 52 Brazilian samples, showing the circulation of just one DEN-2 variant. Phylogenetic analysis using progressive pairwise alignments from 240-nucleotide sequences of the E/NS1 junction in 15 isolates showed that they belong to genotype III (Jamaica genotype).
publishDate 2003
dc.date.none.fl_str_mv 2003-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/30664
url https://www.revistas.usp.br/rimtsp/article/view/30664
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/30664/32548
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 45 No. 1 (2003); 17-21
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 45 Núm. 1 (2003); 17-21
Revista do Instituto de Medicina Tropical de São Paulo; v. 45 n. 1 (2003); 17-21
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
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