Arbitrarily primed PCR fingerprinting of RNA and DNA in Entamoeba histolytica

Detalhes bibliográficos
Autor(a) principal: VALLE, Paulo Renato
Data de Publicação: 2000
Outros Autores: SOUZA, Maria Betânia G., PIRES, Edna M., SILVA, Edward F., GOMES, Maria A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/30451
Resumo: Differences were detected in the gene expression of strains of E. histolytica using RNA (RAP-PCR) and DNA fingerprinting (RAPD). Analysis of the electrophoretic profiles of the gels revealed some polymorphic markers that could be used in the individual characterization of the strains. The 260 bands generated by using five different primers for RAP-PCR, as well as RAPD, were employed in the construction of dendograms. The dendogram obtained based on the RAPD products permitted the distinction of symptomatic and asymptomatic isolates, as well the correlation between the polymorphism exhibited and the virulence of the strains. The dendogram obtained for the RAP-PCR products did not show a correlation with the virulence of the strains but revealed a high degree of intraspecific transcriptional variability that could be related to other biological features, whether or not these are involved in the pathogenesis of amebiasis.
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spelling Arbitrarily primed PCR fingerprinting of RNA and DNA in Entamoeba histolytica Impressões digitais de DNA e RNA através de AP-PCR em Entamoeba histolytica Entamoeba histolytViruleRAP-R Differences were detected in the gene expression of strains of E. histolytica using RNA (RAP-PCR) and DNA fingerprinting (RAPD). Analysis of the electrophoretic profiles of the gels revealed some polymorphic markers that could be used in the individual characterization of the strains. The 260 bands generated by using five different primers for RAP-PCR, as well as RAPD, were employed in the construction of dendograms. The dendogram obtained based on the RAPD products permitted the distinction of symptomatic and asymptomatic isolates, as well the correlation between the polymorphism exhibited and the virulence of the strains. The dendogram obtained for the RAP-PCR products did not show a correlation with the virulence of the strains but revealed a high degree of intraspecific transcriptional variability that could be related to other biological features, whether or not these are involved in the pathogenesis of amebiasis. Diferenças na expressão gênica de cepas de E. histolytica foram obtidas pelo "fingerprinting" de RNA (RAP-PCR) e DNA (RAPD). A análise do perfil eletroforético do gel revelou alguns marcadores polimórficos que poderiam ser usados na caracterização individual das cepas. As 260 bandas geradas pela utilização de cinco primers diferentes, tanto no RAP-PCR, quanto no RAPD foram empregadas na construção de dendogramas. O dendograma obtido com os produtos do RAPD permitiu a distinção das cepas isoladas de pacientes sintomáticos e assintomáticos, além de correlacionar o polimorfismo exibido com a virulência das mesmas. O dendograma obtido com os produtos do RAP-PCR não apresentou correlação com a virulência das cepas, mas revelou uma exuberante variabilidade transcricional intra-específica, que pode estar relacionada a outros caracteres biológicos envolvidos, ou não, na patogênese da amebíase. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2000-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/30451Revista do Instituto de Medicina Tropical de São Paulo; Vol. 42 No. 5 (2000); 249-253 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 42 Núm. 5 (2000); 249-253 Revista do Instituto de Medicina Tropical de São Paulo; v. 42 n. 5 (2000); 249-253 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/30451/32335Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessVALLE, Paulo RenatoSOUZA, Maria Betânia G.PIRES, Edna M.SILVA, Edward F.GOMES, Maria A.2012-07-07T09:36:06Zoai:revistas.usp.br:article/30451Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:13.457988Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Arbitrarily primed PCR fingerprinting of RNA and DNA in Entamoeba histolytica
Impressões digitais de DNA e RNA através de AP-PCR em Entamoeba histolytica
title Arbitrarily primed PCR fingerprinting of RNA and DNA in Entamoeba histolytica
spellingShingle Arbitrarily primed PCR fingerprinting of RNA and DNA in Entamoeba histolytica
VALLE, Paulo Renato
Entamoeba histolyt
Virule
RAP-
R
title_short Arbitrarily primed PCR fingerprinting of RNA and DNA in Entamoeba histolytica
title_full Arbitrarily primed PCR fingerprinting of RNA and DNA in Entamoeba histolytica
title_fullStr Arbitrarily primed PCR fingerprinting of RNA and DNA in Entamoeba histolytica
title_full_unstemmed Arbitrarily primed PCR fingerprinting of RNA and DNA in Entamoeba histolytica
title_sort Arbitrarily primed PCR fingerprinting of RNA and DNA in Entamoeba histolytica
author VALLE, Paulo Renato
author_facet VALLE, Paulo Renato
SOUZA, Maria Betânia G.
PIRES, Edna M.
SILVA, Edward F.
GOMES, Maria A.
author_role author
author2 SOUZA, Maria Betânia G.
PIRES, Edna M.
SILVA, Edward F.
GOMES, Maria A.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv VALLE, Paulo Renato
SOUZA, Maria Betânia G.
PIRES, Edna M.
SILVA, Edward F.
GOMES, Maria A.
dc.subject.por.fl_str_mv Entamoeba histolyt
Virule
RAP-
R
topic Entamoeba histolyt
Virule
RAP-
R
description Differences were detected in the gene expression of strains of E. histolytica using RNA (RAP-PCR) and DNA fingerprinting (RAPD). Analysis of the electrophoretic profiles of the gels revealed some polymorphic markers that could be used in the individual characterization of the strains. The 260 bands generated by using five different primers for RAP-PCR, as well as RAPD, were employed in the construction of dendograms. The dendogram obtained based on the RAPD products permitted the distinction of symptomatic and asymptomatic isolates, as well the correlation between the polymorphism exhibited and the virulence of the strains. The dendogram obtained for the RAP-PCR products did not show a correlation with the virulence of the strains but revealed a high degree of intraspecific transcriptional variability that could be related to other biological features, whether or not these are involved in the pathogenesis of amebiasis.
publishDate 2000
dc.date.none.fl_str_mv 2000-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/30451
url https://www.revistas.usp.br/rimtsp/article/view/30451
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/30451/32335
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 42 No. 5 (2000); 249-253
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 42 Núm. 5 (2000); 249-253
Revista do Instituto de Medicina Tropical de São Paulo; v. 42 n. 5 (2000); 249-253
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
_version_ 1798951642816577536

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