Can domestic dogs be considered a good reservoir of Leishmania (L.) infantum chagasi in an endemic area of nonulcerated cutaneous leishmaniasis in Southern Honduras?
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista do Instituto de Medicina Tropical de São Paulo |
Texto Completo: | https://www.revistas.usp.br/rimtsp/article/view/209950 |
Resumo: | Dogs are considered to be the main domestic reservoir associated with the transmission of Leishmania (L.) infantum chagasi to humans in endemic areas of visceral leishmaniasis in America. However, little is known about the role of canines as a source of infection in endemic areas of nonulcerated cutaneous leishmaniasis (NUCL). Therefore, the objective of the present study was to investigate the role of dogs as a possible reservoir of the parasite in Southern Honduras. Dogs (n = 107) living with individuals affected by NUCL were clinically examined and biological material was collected for parasitological and immunological diagnosis. Most animals showed a healthy appearance and a few presented slight weight loss (64%), alopecia (7%), onychogryphosis (5%) and skin lesions (1%). The overall seroprevalence of Leishmania infection based on the DDP ® quick test and/or in-house ELISA serological test was 41%. The presence of the parasite’s DNA was confirmed in 94% of the dogs; however, the average parasite load in the buffy coat was low at 6.09 parasites/µL, ranging between 0.221 and 50.2. The skin of seropositive dogs examined by histopathology using paraffin sections stained by hematoxylin and immunohistochemistry did not show cutaneous lesions or parasite amastigotes. Based on the absence of parasites in the skin and the low parasite load detected in the buffy coat, it seems that the dog does not represent a good source of infection for the vector in the endemic area of NUCL transmission in Southern Honduras. Other domestic and/or wild animals should be investigated. |
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Can domestic dogs be considered a good reservoir of Leishmania (L.) infantum chagasi in an endemic area of nonulcerated cutaneous leishmaniasis in Southern Honduras?Canine leishmaniasisLeishmania (L.) infantum chagasiNonulcerated cutaneous leishmaniasisDiagnosisHondurasDogs are considered to be the main domestic reservoir associated with the transmission of Leishmania (L.) infantum chagasi to humans in endemic areas of visceral leishmaniasis in America. However, little is known about the role of canines as a source of infection in endemic areas of nonulcerated cutaneous leishmaniasis (NUCL). Therefore, the objective of the present study was to investigate the role of dogs as a possible reservoir of the parasite in Southern Honduras. Dogs (n = 107) living with individuals affected by NUCL were clinically examined and biological material was collected for parasitological and immunological diagnosis. Most animals showed a healthy appearance and a few presented slight weight loss (64%), alopecia (7%), onychogryphosis (5%) and skin lesions (1%). The overall seroprevalence of Leishmania infection based on the DDP ® quick test and/or in-house ELISA serological test was 41%. The presence of the parasite’s DNA was confirmed in 94% of the dogs; however, the average parasite load in the buffy coat was low at 6.09 parasites/µL, ranging between 0.221 and 50.2. The skin of seropositive dogs examined by histopathology using paraffin sections stained by hematoxylin and immunohistochemistry did not show cutaneous lesions or parasite amastigotes. Based on the absence of parasites in the skin and the low parasite load detected in the buffy coat, it seems that the dog does not represent a good source of infection for the vector in the endemic area of NUCL transmission in Southern Honduras. Other domestic and/or wild animals should be investigated.Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2023-03-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/20995010.1590/S1678-9946202365024Revista do Instituto de Medicina Tropical de São Paulo; Vol. 65 (2023); e24Revista do Instituto de Medicina Tropical de São Paulo; v. 65 (2023); e24Revista do Instituto de Medicina Tropical de São Paulo; Vol. 65 (2023); e241678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/209950/192486Copyright (c) 2023 Gabriela Beatriz Rodriguez Segura, Wilfredo Humberto Sosa Ochoa, Vânia Lúcia Ribeiro da Matta, Mercedes Martínez, Carol Rodriguez Tercero, Raquel Romero Gonzalez, Carmen M. Sandoval Pacheco, Gabriela V. Araujo Flores, Fernando Tobias Silveira, Maria Mercedes Rueda Henriquez, Márcia Dalastra Laurentihttps://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccess Segura, Gabriela Beatriz RodriguezOchoa, Wilfredo Humberto Sosa Matta, Vânia Lúcia Ribeiro da Martínez, Mercedes Tercero, Carol Rodriguez Gonzalez, Raquel Romero Pacheco, Carmen M. Sandoval Flores, Gabriela V. AraujoSilveira, Fernando Tobias Henriquez, Maria Mercedes Rueda Laurenti, Márcia Dalastra 2023-12-22T12:47:01Zoai:revistas.usp.br:article/209950Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2023-12-22T12:47:01Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)false |
dc.title.none.fl_str_mv |
Can domestic dogs be considered a good reservoir of Leishmania (L.) infantum chagasi in an endemic area of nonulcerated cutaneous leishmaniasis in Southern Honduras? |
title |
Can domestic dogs be considered a good reservoir of Leishmania (L.) infantum chagasi in an endemic area of nonulcerated cutaneous leishmaniasis in Southern Honduras? |
spellingShingle |
Can domestic dogs be considered a good reservoir of Leishmania (L.) infantum chagasi in an endemic area of nonulcerated cutaneous leishmaniasis in Southern Honduras? Segura, Gabriela Beatriz Rodriguez Canine leishmaniasis Leishmania (L.) infantum chagasi Nonulcerated cutaneous leishmaniasis Diagnosis Honduras |
title_short |
Can domestic dogs be considered a good reservoir of Leishmania (L.) infantum chagasi in an endemic area of nonulcerated cutaneous leishmaniasis in Southern Honduras? |
title_full |
Can domestic dogs be considered a good reservoir of Leishmania (L.) infantum chagasi in an endemic area of nonulcerated cutaneous leishmaniasis in Southern Honduras? |
title_fullStr |
Can domestic dogs be considered a good reservoir of Leishmania (L.) infantum chagasi in an endemic area of nonulcerated cutaneous leishmaniasis in Southern Honduras? |
title_full_unstemmed |
Can domestic dogs be considered a good reservoir of Leishmania (L.) infantum chagasi in an endemic area of nonulcerated cutaneous leishmaniasis in Southern Honduras? |
title_sort |
Can domestic dogs be considered a good reservoir of Leishmania (L.) infantum chagasi in an endemic area of nonulcerated cutaneous leishmaniasis in Southern Honduras? |
author |
Segura, Gabriela Beatriz Rodriguez |
author_facet |
Segura, Gabriela Beatriz Rodriguez Ochoa, Wilfredo Humberto Sosa Matta, Vânia Lúcia Ribeiro da Martínez, Mercedes Tercero, Carol Rodriguez Gonzalez, Raquel Romero Pacheco, Carmen M. Sandoval Flores, Gabriela V. Araujo Silveira, Fernando Tobias Henriquez, Maria Mercedes Rueda Laurenti, Márcia Dalastra |
author_role |
author |
author2 |
Ochoa, Wilfredo Humberto Sosa Matta, Vânia Lúcia Ribeiro da Martínez, Mercedes Tercero, Carol Rodriguez Gonzalez, Raquel Romero Pacheco, Carmen M. Sandoval Flores, Gabriela V. Araujo Silveira, Fernando Tobias Henriquez, Maria Mercedes Rueda Laurenti, Márcia Dalastra |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Segura, Gabriela Beatriz Rodriguez Ochoa, Wilfredo Humberto Sosa Matta, Vânia Lúcia Ribeiro da Martínez, Mercedes Tercero, Carol Rodriguez Gonzalez, Raquel Romero Pacheco, Carmen M. Sandoval Flores, Gabriela V. Araujo Silveira, Fernando Tobias Henriquez, Maria Mercedes Rueda Laurenti, Márcia Dalastra |
dc.subject.por.fl_str_mv |
Canine leishmaniasis Leishmania (L.) infantum chagasi Nonulcerated cutaneous leishmaniasis Diagnosis Honduras |
topic |
Canine leishmaniasis Leishmania (L.) infantum chagasi Nonulcerated cutaneous leishmaniasis Diagnosis Honduras |
description |
Dogs are considered to be the main domestic reservoir associated with the transmission of Leishmania (L.) infantum chagasi to humans in endemic areas of visceral leishmaniasis in America. However, little is known about the role of canines as a source of infection in endemic areas of nonulcerated cutaneous leishmaniasis (NUCL). Therefore, the objective of the present study was to investigate the role of dogs as a possible reservoir of the parasite in Southern Honduras. Dogs (n = 107) living with individuals affected by NUCL were clinically examined and biological material was collected for parasitological and immunological diagnosis. Most animals showed a healthy appearance and a few presented slight weight loss (64%), alopecia (7%), onychogryphosis (5%) and skin lesions (1%). The overall seroprevalence of Leishmania infection based on the DDP ® quick test and/or in-house ELISA serological test was 41%. The presence of the parasite’s DNA was confirmed in 94% of the dogs; however, the average parasite load in the buffy coat was low at 6.09 parasites/µL, ranging between 0.221 and 50.2. The skin of seropositive dogs examined by histopathology using paraffin sections stained by hematoxylin and immunohistochemistry did not show cutaneous lesions or parasite amastigotes. Based on the absence of parasites in the skin and the low parasite load detected in the buffy coat, it seems that the dog does not represent a good source of infection for the vector in the endemic area of NUCL transmission in Southern Honduras. Other domestic and/or wild animals should be investigated. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-03-28 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/209950 10.1590/S1678-9946202365024 |
url |
https://www.revistas.usp.br/rimtsp/article/view/209950 |
identifier_str_mv |
10.1590/S1678-9946202365024 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rimtsp/article/view/209950/192486 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
publisher.none.fl_str_mv |
Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo |
dc.source.none.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 65 (2023); e24 Revista do Instituto de Medicina Tropical de São Paulo; v. 65 (2023); e24 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 65 (2023); e24 1678-9946 0036-4665 reponame:Revista do Instituto de Medicina Tropical de São Paulo instname:Instituto de Medicina Tropical (IMT) instacron:IMT |
instname_str |
Instituto de Medicina Tropical (IMT) |
instacron_str |
IMT |
institution |
IMT |
reponame_str |
Revista do Instituto de Medicina Tropical de São Paulo |
collection |
Revista do Instituto de Medicina Tropical de São Paulo |
repository.name.fl_str_mv |
Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT) |
repository.mail.fl_str_mv |
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1798951636566016000 |