Evaluation of indirect immunofluorescence test for bancroftian filariasis using Wuchereria bancroft microfilariae as the antigen in Recife, Brazil

Detalhes bibliográficos
Autor(a) principal: Dreyer, Gerusa
Data de Publicação: 1991
Outros Autores: Andrade, Luiz, Espírito Santo, Marlene, Medeiros, Zulma, Moura, Isolda, Tenório, Jocelene, Rocha, Abraham, Casimiro, Maria Itecir, Galdino, Eliane, Dreyer, Elisabeth, Béliz, Fátima, Rangel, Antonio, Coutinho, Amaury
Tipo de documento: Artigo
Idioma: por
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/28860
Resumo: The authors analysed the indirect immunofluorescence assay, for the diagnosis of bancroftian filariasis using papain treated W. bancrofti microfilariae as antigen, widely used in Recife - Brazil. Sera from 50 patients with several clinical forms of the disease including asymptomatic carriers, tropical pulmonary eosinophilia, elephantiasis, filarial fever and chyluria were analysed. For the control group, 50 individuals were selected, living at least 5 years in endemic area, with neither previous DEC treatment nor clinical-laboratory evidences of the disease, called normals endemic. The sensitivity and specificity were analised taking into account different cut off values. It was not possible to differentiate infected individuals from the control group. It was not even possible to establish any correlation with IMF titers among different clinical presentation of the disease. Crossed reactions with various intestinal helminths were considered, but no relationship was found.
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spelling Evaluation of indirect immunofluorescence test for bancroftian filariasis using Wuchereria bancroft microfilariae as the antigen in Recife, Brazil Avaliação do teste de imunofluorescência indireta para diagnóstico da filariose bancroftiana usando a microfilária de W. bancrofti como antígeno, em Recife-PE, Brasil W. bancroftiimunodiagnósticoimunofluorescência indiretaresposta humoralfilariose linfática The authors analysed the indirect immunofluorescence assay, for the diagnosis of bancroftian filariasis using papain treated W. bancrofti microfilariae as antigen, widely used in Recife - Brazil. Sera from 50 patients with several clinical forms of the disease including asymptomatic carriers, tropical pulmonary eosinophilia, elephantiasis, filarial fever and chyluria were analysed. For the control group, 50 individuals were selected, living at least 5 years in endemic area, with neither previous DEC treatment nor clinical-laboratory evidences of the disease, called normals endemic. The sensitivity and specificity were analised taking into account different cut off values. It was not possible to differentiate infected individuals from the control group. It was not even possible to establish any correlation with IMF titers among different clinical presentation of the disease. Crossed reactions with various intestinal helminths were considered, but no relationship was found. Analisou-se o teste de imunofluorescência indireta com microfilárias de W. bancrofti tratadas pela papaína, como antígeno, amplamente utilizado em Recife para o imunodiagnóstico da filariose linfática. Foram testados soros de 50 pacientes portadores das diversas formas clínicas da doença, incluindo microfilaremia assintomática, eosinofilia pulmonar tropical, elefantíase de membros inferiores, linfagite aguda e quilúria. Para o grupo controle, foram selecionados 50 indivíduos vivendo pelo menos há 5 anos em área endêmica, sem nenhuma evidencia clínica e/ou laboratorial da doença, constituindo os chamados endêmicos normais. A sensibilidade e especificidade do teste, segundo diferentes pontos de corte, mostraram a impossibilidade de diferenciação entre o grupo controle e o grupo sabidamente infectado. Também não foi possível estabelecer correlação entre os títulos encontrados e as diferentes formas clínicas. Foi considerada a existência de reações cruzadas relacionadas a helmintíases intestinais, porém nenhuma relação direta foi encontrada. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1991-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/28860Revista do Instituto de Medicina Tropical de São Paulo; Vol. 33 No. 5 (1991); 397-402 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 33 Núm. 5 (1991); 397-402 Revista do Instituto de Medicina Tropical de São Paulo; v. 33 n. 5 (1991); 397-402 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTporhttps://www.revistas.usp.br/rimtsp/article/view/28860/30713Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessDreyer, GerusaAndrade, LuizEspírito Santo, MarleneMedeiros, ZulmaMoura, IsoldaTenório, JoceleneRocha, AbrahamCasimiro, Maria ItecirGaldino, ElianeDreyer, ElisabethBéliz, FátimaRangel, AntonioCoutinho, Amaury2012-07-02T01:31:09Zoai:revistas.usp.br:article/28860Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2012-07-02T01:31:09Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)false
dc.title.none.fl_str_mv Evaluation of indirect immunofluorescence test for bancroftian filariasis using Wuchereria bancroft microfilariae as the antigen in Recife, Brazil
Avaliação do teste de imunofluorescência indireta para diagnóstico da filariose bancroftiana usando a microfilária de W. bancrofti como antígeno, em Recife-PE, Brasil
title Evaluation of indirect immunofluorescence test for bancroftian filariasis using Wuchereria bancroft microfilariae as the antigen in Recife, Brazil
spellingShingle Evaluation of indirect immunofluorescence test for bancroftian filariasis using Wuchereria bancroft microfilariae as the antigen in Recife, Brazil
Dreyer, Gerusa
W. bancrofti
imunodiagnóstico
imunofluorescência indireta
resposta humoral
filariose linfática
title_short Evaluation of indirect immunofluorescence test for bancroftian filariasis using Wuchereria bancroft microfilariae as the antigen in Recife, Brazil
title_full Evaluation of indirect immunofluorescence test for bancroftian filariasis using Wuchereria bancroft microfilariae as the antigen in Recife, Brazil
title_fullStr Evaluation of indirect immunofluorescence test for bancroftian filariasis using Wuchereria bancroft microfilariae as the antigen in Recife, Brazil
title_full_unstemmed Evaluation of indirect immunofluorescence test for bancroftian filariasis using Wuchereria bancroft microfilariae as the antigen in Recife, Brazil
title_sort Evaluation of indirect immunofluorescence test for bancroftian filariasis using Wuchereria bancroft microfilariae as the antigen in Recife, Brazil
author Dreyer, Gerusa
author_facet Dreyer, Gerusa
Andrade, Luiz
Espírito Santo, Marlene
Medeiros, Zulma
Moura, Isolda
Tenório, Jocelene
Rocha, Abraham
Casimiro, Maria Itecir
Galdino, Eliane
Dreyer, Elisabeth
Béliz, Fátima
Rangel, Antonio
Coutinho, Amaury
author_role author
author2 Andrade, Luiz
Espírito Santo, Marlene
Medeiros, Zulma
Moura, Isolda
Tenório, Jocelene
Rocha, Abraham
Casimiro, Maria Itecir
Galdino, Eliane
Dreyer, Elisabeth
Béliz, Fátima
Rangel, Antonio
Coutinho, Amaury
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Dreyer, Gerusa
Andrade, Luiz
Espírito Santo, Marlene
Medeiros, Zulma
Moura, Isolda
Tenório, Jocelene
Rocha, Abraham
Casimiro, Maria Itecir
Galdino, Eliane
Dreyer, Elisabeth
Béliz, Fátima
Rangel, Antonio
Coutinho, Amaury
dc.subject.por.fl_str_mv W. bancrofti
imunodiagnóstico
imunofluorescência indireta
resposta humoral
filariose linfática
topic W. bancrofti
imunodiagnóstico
imunofluorescência indireta
resposta humoral
filariose linfática
description The authors analysed the indirect immunofluorescence assay, for the diagnosis of bancroftian filariasis using papain treated W. bancrofti microfilariae as antigen, widely used in Recife - Brazil. Sera from 50 patients with several clinical forms of the disease including asymptomatic carriers, tropical pulmonary eosinophilia, elephantiasis, filarial fever and chyluria were analysed. For the control group, 50 individuals were selected, living at least 5 years in endemic area, with neither previous DEC treatment nor clinical-laboratory evidences of the disease, called normals endemic. The sensitivity and specificity were analised taking into account different cut off values. It was not possible to differentiate infected individuals from the control group. It was not even possible to establish any correlation with IMF titers among different clinical presentation of the disease. Crossed reactions with various intestinal helminths were considered, but no relationship was found.
publishDate 1991
dc.date.none.fl_str_mv 1991-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/28860
url https://www.revistas.usp.br/rimtsp/article/view/28860
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/28860/30713
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 33 No. 5 (1991); 397-402
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 33 Núm. 5 (1991); 397-402
Revista do Instituto de Medicina Tropical de São Paulo; v. 33 n. 5 (1991); 397-402
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
_version_ 1752129949193469952

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