Extended-spectrum beta-lactamases among Enterobacteriaceae isolated in a public hospital in Brazil

• Autor(a) principal: Dropa, Milena
• Data de Publicação: 2009
• Outros Autores: Balsalobre, Livia C., Lincopan, Nilton, Mamizuka, Elsa M., Murakami, Thays, Cassettari, Valéria C., Franco, Fábio, Guida, Stella M., Balabakis, Angelica J., Passadore, Lilian F., Santos, Silvia R., Matté, Glavur R., Matté, Maria H.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
• Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/31274
• topic: ESBL; Enterobacteriaceae; Klebsiella pneumoniae; Pulsed Field Gel Electrophoresis; Antimicrobial resistance

Extended-spectrum β-lactamases (ESBL) in enterobacteria are recognized worldwide as a great hospital problem. In this study, 127 ESBL-producing Enterobacteriaceae isolated in one year from inpatients and outpatients at a public teaching hospital at São Paulo, Brazil, were submitted to analysis by PCR with specific primers for blaSHV, blaTEM and blaCTX-M genes. From the 127 isolates, 96 (75.6%) Klebsiella pneumoniae, 12 (9.3%) Escherichia coli, 8 (6.2%) Morganella morganii, 3 (2.3%) Proteus mirabilis, 2 (1.6%) Klebsiella oxytoca, 2 (1.6%) Providencia rettgeri, 2 (1.6%) Providencia stuartti, 1 (0.8%) Enterobacter aerogenes and 1 (0.8%) Enterobacter cloacae were identified as ESBL producers. BlaSHV, blaTEM and blaCTX-M were detected in 63%, 17.3% and 33.9% strains, respectively. Pulsed field gel eletrophoresis genotyping of K. pneumoniae revealed four main molecular patterns and 29 unrelated profiles. PCR results showed a high variety of ESBL groups among strains, in nine different species. The results suggest the spread of resistance genes among genetically different strains of ESBL-producing K. pneumoniae in some hospital wards, and also that some strongly related strains were identified in different hospital wards, suggesting clonal spread in the institutional environment.