Clarithromycin-resistant H. pylori primary strains and virulence genotypes in the Northeastern region of Brazil

Detalhes bibliográficos
Autor(a) principal: Benigno, Tiago Gomes da Silva
Data de Publicação: 2022
Outros Autores: Ribeiro Junior, Howard Lopes, Azevedo, Orleâncio Gomes Ripardo de, Pinheiro, Ronald Feitosa, Oliveira, Roberta Taiane Germano de, Maciel, Felipe Silva, Oliveira, Edson Luiz de, Queiroz, Dulciene Maria Magalhães, Braga, Lucia Libanez Bessa Campelo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/200086
Resumo: The increase of H. pylori resistance to clarithromycin is a concern. This study evaluated the prevalence of H. pylori’s primary resistance to clarithromycin and its association with virulence factors in adult dyspeptic patients and asymptomatic children. The gastric mucosa from patients (153 gastritis, 24 gastric cancer, 21 peptic ulcer) and gastric juice obtained by string test from 24 H. pylori and 23S rRNA positive asymptomatic children were included. The clarithromycin resistance was assessed by TaqMan RT-PCR 23S rRNA point mutations, A2142G and/or A2143G, and H. pylori virulence markers by PCR. Overall, the clarithromycin resistance was 14.4% (32/222), 14.2% in adults, and 12% in children, whereas origin, gender, and disease were not distinctive factors. The most prevalent point mutation was A2143G (62.5%). The point mutation was significantly less frequent in cagA-positive (11.4%) than in cagA-negative (23.6%) strains (p=0.03 OR = 0.4 95%CI = 0.19 - 0.91) as well as in cagE-positive (10.2%), cagE-negative (21.2%) (p=0.03 OR: 0.4 I.C:0.20-0.91). No difference was found in iceA or vacA alleles genotypes. Primary resistance to clarithromycin was lower than that reported in Southeast Brazil. The cagA and cagE positive H. pylori samples have few point mutations suggesting that individuals infected with virulent strains may be more susceptible to anti-H. pylori treatment.
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spelling Clarithromycin-resistant H. pylori primary strains and virulence genotypes in the Northeastern region of BrazilH. pyloriVirulence factorsClarithromycin-resistanceThe increase of H. pylori resistance to clarithromycin is a concern. This study evaluated the prevalence of H. pylori’s primary resistance to clarithromycin and its association with virulence factors in adult dyspeptic patients and asymptomatic children. The gastric mucosa from patients (153 gastritis, 24 gastric cancer, 21 peptic ulcer) and gastric juice obtained by string test from 24 H. pylori and 23S rRNA positive asymptomatic children were included. The clarithromycin resistance was assessed by TaqMan RT-PCR 23S rRNA point mutations, A2142G and/or A2143G, and H. pylori virulence markers by PCR. Overall, the clarithromycin resistance was 14.4% (32/222), 14.2% in adults, and 12% in children, whereas origin, gender, and disease were not distinctive factors. The most prevalent point mutation was A2143G (62.5%). The point mutation was significantly less frequent in cagA-positive (11.4%) than in cagA-negative (23.6%) strains (p=0.03 OR = 0.4 95%CI = 0.19 - 0.91) as well as in cagE-positive (10.2%), cagE-negative (21.2%) (p=0.03 OR: 0.4 I.C:0.20-0.91). No difference was found in iceA or vacA alleles genotypes. Primary resistance to clarithromycin was lower than that reported in Southeast Brazil. The cagA and cagE positive H. pylori samples have few point mutations suggesting that individuals infected with virulent strains may be more susceptible to anti-H. pylori treatment.Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2022-07-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/20008610.1590/S1678-9946202264047 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 64 (2022); e47Revista do Instituto de Medicina Tropical de São Paulo; Vol. 64 (2022); e47Revista do Instituto de Medicina Tropical de São Paulo; v. 64 (2022); e471678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/200086/184339Copyright (c) 2022 Tiago Gomes da Silva Benigno, Howard Lopes Ribeiro Junior, Orleâncio Gomes Ripardo de Azevedo, Ronald Feitosa Pinheiro, Roberta Taiane Germano de Oliveira, Felipe Silva Maciel, Edson Luiz de Oliveira, Dulciene Maria Magalhães Queiroz, Lucia Libanez Bessa Campelo Bragahttps://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccess Benigno, Tiago Gomes da SilvaRibeiro Junior, Howard Lopes Azevedo, Orleâncio Gomes Ripardo dePinheiro, Ronald Feitosa Oliveira, Roberta Taiane Germano de Maciel, Felipe Silva Oliveira, Edson Luiz de Queiroz, Dulciene Maria Magalhães Braga, Lucia Libanez Bessa Campelo 2022-10-10T13:01:46Zoai:revistas.usp.br:article/200086Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:53:36.801122Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Clarithromycin-resistant H. pylori primary strains and virulence genotypes in the Northeastern region of Brazil
title Clarithromycin-resistant H. pylori primary strains and virulence genotypes in the Northeastern region of Brazil
spellingShingle Clarithromycin-resistant H. pylori primary strains and virulence genotypes in the Northeastern region of Brazil
Benigno, Tiago Gomes da Silva
H. pylori
Virulence factors
Clarithromycin-resistance
title_short Clarithromycin-resistant H. pylori primary strains and virulence genotypes in the Northeastern region of Brazil
title_full Clarithromycin-resistant H. pylori primary strains and virulence genotypes in the Northeastern region of Brazil
title_fullStr Clarithromycin-resistant H. pylori primary strains and virulence genotypes in the Northeastern region of Brazil
title_full_unstemmed Clarithromycin-resistant H. pylori primary strains and virulence genotypes in the Northeastern region of Brazil
title_sort Clarithromycin-resistant H. pylori primary strains and virulence genotypes in the Northeastern region of Brazil
author Benigno, Tiago Gomes da Silva
author_facet Benigno, Tiago Gomes da Silva
Ribeiro Junior, Howard Lopes
Azevedo, Orleâncio Gomes Ripardo de
Pinheiro, Ronald Feitosa
Oliveira, Roberta Taiane Germano de
Maciel, Felipe Silva
Oliveira, Edson Luiz de
Queiroz, Dulciene Maria Magalhães
Braga, Lucia Libanez Bessa Campelo
author_role author
author2 Ribeiro Junior, Howard Lopes
Azevedo, Orleâncio Gomes Ripardo de
Pinheiro, Ronald Feitosa
Oliveira, Roberta Taiane Germano de
Maciel, Felipe Silva
Oliveira, Edson Luiz de
Queiroz, Dulciene Maria Magalhães
Braga, Lucia Libanez Bessa Campelo
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Benigno, Tiago Gomes da Silva
Ribeiro Junior, Howard Lopes
Azevedo, Orleâncio Gomes Ripardo de
Pinheiro, Ronald Feitosa
Oliveira, Roberta Taiane Germano de
Maciel, Felipe Silva
Oliveira, Edson Luiz de
Queiroz, Dulciene Maria Magalhães
Braga, Lucia Libanez Bessa Campelo
dc.subject.por.fl_str_mv H. pylori
Virulence factors
Clarithromycin-resistance
topic H. pylori
Virulence factors
Clarithromycin-resistance
description The increase of H. pylori resistance to clarithromycin is a concern. This study evaluated the prevalence of H. pylori’s primary resistance to clarithromycin and its association with virulence factors in adult dyspeptic patients and asymptomatic children. The gastric mucosa from patients (153 gastritis, 24 gastric cancer, 21 peptic ulcer) and gastric juice obtained by string test from 24 H. pylori and 23S rRNA positive asymptomatic children were included. The clarithromycin resistance was assessed by TaqMan RT-PCR 23S rRNA point mutations, A2142G and/or A2143G, and H. pylori virulence markers by PCR. Overall, the clarithromycin resistance was 14.4% (32/222), 14.2% in adults, and 12% in children, whereas origin, gender, and disease were not distinctive factors. The most prevalent point mutation was A2143G (62.5%). The point mutation was significantly less frequent in cagA-positive (11.4%) than in cagA-negative (23.6%) strains (p=0.03 OR = 0.4 95%CI = 0.19 - 0.91) as well as in cagE-positive (10.2%), cagE-negative (21.2%) (p=0.03 OR: 0.4 I.C:0.20-0.91). No difference was found in iceA or vacA alleles genotypes. Primary resistance to clarithromycin was lower than that reported in Southeast Brazil. The cagA and cagE positive H. pylori samples have few point mutations suggesting that individuals infected with virulent strains may be more susceptible to anti-H. pylori treatment.
publishDate 2022
dc.date.none.fl_str_mv 2022-07-14
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/200086
10.1590/S1678-9946202264047
url https://www.revistas.usp.br/rimtsp/article/view/200086
identifier_str_mv 10.1590/S1678-9946202264047
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/200086/184339
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 64 (2022); e47
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 64 (2022); e47
Revista do Instituto de Medicina Tropical de São Paulo; v. 64 (2022); e47
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
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