Borrelia burgdorferi antibodies in dogs from Cotia county, São Paulo State, Brazil

Detalhes bibliográficos
Autor(a) principal: JOPPERT, Adriana Marques
Data de Publicação: 2001
Outros Autores: HAGIWARA, Mitika Kuribayashi, YOSHINARI, Natalino Hajime
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/30541
Resumo: Dogs sera samples collected from Cotia County, São Paulo were tested using indirect immunoenzymatic test (ELISA) in order to study Lyme disease serology in dogs. ELISA method was standardized and G39/40 North American strain of Borrelia burgdorferi was used as antigen. Positive results were confirmed employing the Western blotting technique. Because of the possibility of cross-reactions, sera were also tested for different serological strains of Leptospira interrogans and L. biflexa using microscopic sera agglutination test. Twenty-three of 237 (9.7%) serum samples were positive in the ELISA; 20 of them (86.9%) were confirmed by the Western blotting, what suggests that Cotia may be a risk area for Lyme disease. Although 4 samples (1.7%) were positive for Lyme disease and leptospirosis, no correlation was found between the results (X² = 0.725; p = 0.394) what suggests absence of serological cross reactivity.
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spelling Borrelia burgdorferi antibodies in dogs from Cotia county, São Paulo State, Brazil Pesquisa de anticorpos anti-Borrelia burgdorferi em cães da região de Cotia, São Paulo, Brasil Lyme diseaseBorrelia burgdorferiDogsELISABrazil Dogs sera samples collected from Cotia County, São Paulo were tested using indirect immunoenzymatic test (ELISA) in order to study Lyme disease serology in dogs. ELISA method was standardized and G39/40 North American strain of Borrelia burgdorferi was used as antigen. Positive results were confirmed employing the Western blotting technique. Because of the possibility of cross-reactions, sera were also tested for different serological strains of Leptospira interrogans and L. biflexa using microscopic sera agglutination test. Twenty-three of 237 (9.7%) serum samples were positive in the ELISA; 20 of them (86.9%) were confirmed by the Western blotting, what suggests that Cotia may be a risk area for Lyme disease. Although 4 samples (1.7%) were positive for Lyme disease and leptospirosis, no correlation was found between the results (X² = 0.725; p = 0.394) what suggests absence of serological cross reactivity. Com a finalidade de estudar a ocorrência da doença de Lyme em cães no Brasil, o teste imunoenzimático (ELISA) indireto, utilizado para o diagnóstico da doença no homem, foi padronizado para a espécie canina. Utilizou-se como antígeno a cepa americana de Borrelia burgdorferi G 39/40. Os soros de cães procedentes da região de Cotia, área de risco para ocorrência da doença de Lyme, foram testados e os resultados positivos foram confirmados através da técnica de "Western blotting". Para investigação de possíveis reações cruzadas, os soros foram também testados para diferentes variantes sorológicas de Leptospira interrogans e L. biflexa pela técnica de soroaglutinação microscópica. Dos 237 soros testados pela técnica de ELISA, 23 (9,7%) foram positivos, sendo que 20 (86,9%) destes soros foram também positivos pela técnica de "Western blotting", sugerindo que a região de Cotia seja área de risco para doença de Lyme. Embora 4 (1,7%) dos soros testados apresentassem positividade para doença de Lyme e para leptospirose concomitantemente, não foi observada associação entre estes resultados (X² = 0,725 e p = 0,394), sugerindo ausência de reatividade sorológica cruzada entre as duas enfermidades. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2001-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/30541Revista do Instituto de Medicina Tropical de São Paulo; Vol. 43 No. 5 (2001); 251-255 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 43 Núm. 5 (2001); 251-255 Revista do Instituto de Medicina Tropical de São Paulo; v. 43 n. 5 (2001); 251-255 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/30541/32425Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessJOPPERT, Adriana MarquesHAGIWARA, Mitika KuribayashiYOSHINARI, Natalino Hajime2012-07-07T13:35:12Zoai:revistas.usp.br:article/30541Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:51:19.606915Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Borrelia burgdorferi antibodies in dogs from Cotia county, São Paulo State, Brazil
Pesquisa de anticorpos anti-Borrelia burgdorferi em cães da região de Cotia, São Paulo, Brasil
title Borrelia burgdorferi antibodies in dogs from Cotia county, São Paulo State, Brazil
spellingShingle Borrelia burgdorferi antibodies in dogs from Cotia county, São Paulo State, Brazil
JOPPERT, Adriana Marques
Lyme disease
Borrelia burgdorferi
Dogs
ELISA
Brazil
title_short Borrelia burgdorferi antibodies in dogs from Cotia county, São Paulo State, Brazil
title_full Borrelia burgdorferi antibodies in dogs from Cotia county, São Paulo State, Brazil
title_fullStr Borrelia burgdorferi antibodies in dogs from Cotia county, São Paulo State, Brazil
title_full_unstemmed Borrelia burgdorferi antibodies in dogs from Cotia county, São Paulo State, Brazil
title_sort Borrelia burgdorferi antibodies in dogs from Cotia county, São Paulo State, Brazil
author JOPPERT, Adriana Marques
author_facet JOPPERT, Adriana Marques
HAGIWARA, Mitika Kuribayashi
YOSHINARI, Natalino Hajime
author_role author
author2 HAGIWARA, Mitika Kuribayashi
YOSHINARI, Natalino Hajime
author2_role author
author
dc.contributor.author.fl_str_mv JOPPERT, Adriana Marques
HAGIWARA, Mitika Kuribayashi
YOSHINARI, Natalino Hajime
dc.subject.por.fl_str_mv Lyme disease
Borrelia burgdorferi
Dogs
ELISA
Brazil
topic Lyme disease
Borrelia burgdorferi
Dogs
ELISA
Brazil
description Dogs sera samples collected from Cotia County, São Paulo were tested using indirect immunoenzymatic test (ELISA) in order to study Lyme disease serology in dogs. ELISA method was standardized and G39/40 North American strain of Borrelia burgdorferi was used as antigen. Positive results were confirmed employing the Western blotting technique. Because of the possibility of cross-reactions, sera were also tested for different serological strains of Leptospira interrogans and L. biflexa using microscopic sera agglutination test. Twenty-three of 237 (9.7%) serum samples were positive in the ELISA; 20 of them (86.9%) were confirmed by the Western blotting, what suggests that Cotia may be a risk area for Lyme disease. Although 4 samples (1.7%) were positive for Lyme disease and leptospirosis, no correlation was found between the results (X² = 0.725; p = 0.394) what suggests absence of serological cross reactivity.
publishDate 2001
dc.date.none.fl_str_mv 2001-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/30541
url https://www.revistas.usp.br/rimtsp/article/view/30541
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/30541/32425
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 43 No. 5 (2001); 251-255
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 43 Núm. 5 (2001); 251-255
Revista do Instituto de Medicina Tropical de São Paulo; v. 43 n. 5 (2001); 251-255
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
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