Isolation of yellow fever virus (YFV) from naturally infectied Haemagogus (Conopostegus) leucocelaenus (diptera, cukicudae) in São Paulo State, Brazil, 2009

• Autor(a) principal: Souza, Renato Pereira de
• Data de Publicação: 2011
• Outros Autores: Petrella, Selma, Coimbra, Terezinha Lisieux Moraes, Maeda, Adriana Yurika, Rocco, Iray Maria, Bisordi, Ivani, Silveira, Vivian Regina, Pereira, Luiz Eloy, Suzuki, Akemi, Silva, Sarai Joaquim dos Santos, Silva, Fernanda Gisele, Salvador, Felipe Scassi, Tubaki, Rosa Maria, Menezes, Regiane Tironi, Pereira, Mariza, Bergo, Eduardo Sterlino, Hoffmann, Roberto Colozza, Spinola, Roberta Maria Fernandes, Tengan, Cílea Hatsumi, Siciliano, Melissa Mascheratti
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
• Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/31392
• topic: Yellow fever; Haemagogus leucocelaenus; Arbovirus

After detecting the death of Howlers monkeys (genus Alouatta) and isolation of yellow fever virus (YFV) in Buri county, São Paulo, Brazil, an entomological research study in the field was started. A YFV strain was isolated from newborn Swiss mice and cultured cells of Aedes albopictus - C6/36, from a pool of six Haemagogus (Conopostegus) leucocelaenus (Hg. leucocelaenus) mosquitoes (Dyar & Shannon) collected at the study site. Virus RNA fragment was amplified by RT-PCR and sequenced. The MCC Tree generated showed that the isolated strain is related to the South American I genotype, in a monophyletic clade containing isolates from recent 2008-2010 epidemics and epizootics in Brazil. Statistical analysis commonly used were calculated to characterize the sample in relation to diversity and dominance and indicated a pattern of dominance of one or a few species. Hg. leucocelaenus was found infected in Rio Grande do Sul State as well. In São Paulo State, this is the first detection of YFV in Hg. leucocelaenus.