Deep sequencing applied to the analysis of viromes in patients with beta-thalassemia

Detalhes bibliográficos
Autor(a) principal: Valença, Ian Nunes
Data de Publicação: 2021
Outros Autores: Santos, Rafael Bezerra dos, Peronni, Kamila Chagas, Sauvage, Virginie, Vandenbogaert, Mathias, Caro, Valérie, Silva Junior, Wilson Araújo da, Covas, Dimas Tadeu, Silva-Pinto, Ana Cristina, Laperche, Syria, Kashima, Simone, Slavov, Svetoslav Nanev
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/187111
Resumo: To date, blood banks apply routine diagnosis to a specific spectrum of transfusion-transmitted viruses. Even though this measure is considered highly efficient to control their transmission, the threat imposed by emerging viruses is increasing globally, which can impact transfusion safety, especially in the light of the accelerated viral discovery by novel sequencing technologies. One of the most important groups of patients, who may indicate the presence of emerging viruses in the field of blood transfusion, is the group of individuals who receive multiple transfusions due to hereditary hemoglobinopathies. It is possible that they harbor unknown or unsuspected parenterally-transmitted viruses. In order to elucidate this, nucleic acids from 30 patients with beta-thalassemia were analyzed by Illumina next-generation sequencing and bioinformatics analysis. Three major viral families: Anelloviridae, Flaviviridae and Hepadnaviridae were identified. Among them, anelloviruses were the most representative, being detected with high number of reads in all tested samples. Human Pegivirus 1 (HPgV-1, or GBV-C), Hepatitis B Virus (HBV) and Hepatitis C Virus (HCV) were also identified. HBV and HCV detection was expected due to the high seroprevalence in patients with beta thalassemia. Our results do not confirm the presence of emerging or unsuspected viruses threatening the transfusion safety at present, but can be used to actively search for viruses that threaten blood transfusion safety. We believe that the application of viral metagenomics in multiple-transfused patients is highly useful to monitor possible viral transfusion threats and for the annotation of their virome composition.
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spelling Deep sequencing applied to the analysis of viromes in patients with beta-thalassemiaMetagenomicsNext-generation sequencingBeta-thalassemiaViromeHemotherapyEmerging virusesTo date, blood banks apply routine diagnosis to a specific spectrum of transfusion-transmitted viruses. Even though this measure is considered highly efficient to control their transmission, the threat imposed by emerging viruses is increasing globally, which can impact transfusion safety, especially in the light of the accelerated viral discovery by novel sequencing technologies. One of the most important groups of patients, who may indicate the presence of emerging viruses in the field of blood transfusion, is the group of individuals who receive multiple transfusions due to hereditary hemoglobinopathies. It is possible that they harbor unknown or unsuspected parenterally-transmitted viruses. In order to elucidate this, nucleic acids from 30 patients with beta-thalassemia were analyzed by Illumina next-generation sequencing and bioinformatics analysis. Three major viral families: Anelloviridae, Flaviviridae and Hepadnaviridae were identified. Among them, anelloviruses were the most representative, being detected with high number of reads in all tested samples. Human Pegivirus 1 (HPgV-1, or GBV-C), Hepatitis B Virus (HBV) and Hepatitis C Virus (HCV) were also identified. HBV and HCV detection was expected due to the high seroprevalence in patients with beta thalassemia. Our results do not confirm the presence of emerging or unsuspected viruses threatening the transfusion safety at present, but can be used to actively search for viruses that threaten blood transfusion safety. We believe that the application of viral metagenomics in multiple-transfused patients is highly useful to monitor possible viral transfusion threats and for the annotation of their virome composition.Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo2021-06-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/18711110.1590/S1678-9946202163040Revista do Instituto de Medicina Tropical de São Paulo; Vol. 63 (2021); e40Revista do Instituto de Medicina Tropical de São Paulo; Vol. 63 (2021); e40Revista do Instituto de Medicina Tropical de São Paulo; v. 63 (2021); e401678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/187111/172869Copyright (c) 2021 Ian Nunes Valença, Rafael Bezerra dos Santos, Kamila Chagas Peronni, Virginie Sauvage, Mathias Vandenbogaert, Valérie Caro, Wilson Araújo da Silva Junior, Dimas Tadeu Covas, Ana Cristina Silva-Pinto, Syria Laperche, Simone Kashima, Svetoslav Nanev Slavovhttps://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccess Valença, Ian NunesSantos, Rafael Bezerra dos Peronni, Kamila Chagas Sauvage, Virginie Vandenbogaert, Mathias Caro, Valérie Silva Junior, Wilson Araújo da Covas, Dimas Tadeu Silva-Pinto, Ana Cristina Laperche, Syria Kashima, Simone Slavov, Svetoslav Nanev 2022-05-16T13:44:35Zoai:revistas.usp.br:article/187111Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:52:58.157693Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Deep sequencing applied to the analysis of viromes in patients with beta-thalassemia
title Deep sequencing applied to the analysis of viromes in patients with beta-thalassemia
spellingShingle Deep sequencing applied to the analysis of viromes in patients with beta-thalassemia
Valença, Ian Nunes
Metagenomics
Next-generation sequencing
Beta-thalassemia
Virome
Hemotherapy
Emerging viruses
title_short Deep sequencing applied to the analysis of viromes in patients with beta-thalassemia
title_full Deep sequencing applied to the analysis of viromes in patients with beta-thalassemia
title_fullStr Deep sequencing applied to the analysis of viromes in patients with beta-thalassemia
title_full_unstemmed Deep sequencing applied to the analysis of viromes in patients with beta-thalassemia
title_sort Deep sequencing applied to the analysis of viromes in patients with beta-thalassemia
author Valença, Ian Nunes
author_facet Valença, Ian Nunes
Santos, Rafael Bezerra dos
Peronni, Kamila Chagas
Sauvage, Virginie
Vandenbogaert, Mathias
Caro, Valérie
Silva Junior, Wilson Araújo da
Covas, Dimas Tadeu
Silva-Pinto, Ana Cristina
Laperche, Syria
Kashima, Simone
Slavov, Svetoslav Nanev
author_role author
author2 Santos, Rafael Bezerra dos
Peronni, Kamila Chagas
Sauvage, Virginie
Vandenbogaert, Mathias
Caro, Valérie
Silva Junior, Wilson Araújo da
Covas, Dimas Tadeu
Silva-Pinto, Ana Cristina
Laperche, Syria
Kashima, Simone
Slavov, Svetoslav Nanev
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Valença, Ian Nunes
Santos, Rafael Bezerra dos
Peronni, Kamila Chagas
Sauvage, Virginie
Vandenbogaert, Mathias
Caro, Valérie
Silva Junior, Wilson Araújo da
Covas, Dimas Tadeu
Silva-Pinto, Ana Cristina
Laperche, Syria
Kashima, Simone
Slavov, Svetoslav Nanev
dc.subject.por.fl_str_mv Metagenomics
Next-generation sequencing
Beta-thalassemia
Virome
Hemotherapy
Emerging viruses
topic Metagenomics
Next-generation sequencing
Beta-thalassemia
Virome
Hemotherapy
Emerging viruses
description To date, blood banks apply routine diagnosis to a specific spectrum of transfusion-transmitted viruses. Even though this measure is considered highly efficient to control their transmission, the threat imposed by emerging viruses is increasing globally, which can impact transfusion safety, especially in the light of the accelerated viral discovery by novel sequencing technologies. One of the most important groups of patients, who may indicate the presence of emerging viruses in the field of blood transfusion, is the group of individuals who receive multiple transfusions due to hereditary hemoglobinopathies. It is possible that they harbor unknown or unsuspected parenterally-transmitted viruses. In order to elucidate this, nucleic acids from 30 patients with beta-thalassemia were analyzed by Illumina next-generation sequencing and bioinformatics analysis. Three major viral families: Anelloviridae, Flaviviridae and Hepadnaviridae were identified. Among them, anelloviruses were the most representative, being detected with high number of reads in all tested samples. Human Pegivirus 1 (HPgV-1, or GBV-C), Hepatitis B Virus (HBV) and Hepatitis C Virus (HCV) were also identified. HBV and HCV detection was expected due to the high seroprevalence in patients with beta thalassemia. Our results do not confirm the presence of emerging or unsuspected viruses threatening the transfusion safety at present, but can be used to actively search for viruses that threaten blood transfusion safety. We believe that the application of viral metagenomics in multiple-transfused patients is highly useful to monitor possible viral transfusion threats and for the annotation of their virome composition.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-14
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/187111
10.1590/S1678-9946202163040
url https://www.revistas.usp.br/rimtsp/article/view/187111
identifier_str_mv 10.1590/S1678-9946202163040
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/187111/172869
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 63 (2021); e40
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 63 (2021); e40
Revista do Instituto de Medicina Tropical de São Paulo; v. 63 (2021); e40
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
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reponame_str Revista do Instituto de Medicina Tropical de São Paulo
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