Trypanosoma cruzi epimastigotes are able to store and mobilize high amounts of cholesterol in reservosome lipid inclusions
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional do INMETRO |
Texto Completo: | http://hdl.handle.net/10926/1738 |
Resumo: | Background: Reservosomes are lysosome-related organelles found in Trypanosoma cruzi epimastigotes. They represent the last step in epimastigote endocytic route, accumulating a set of proteins and enzymes related to protein digestion and lipid metabolism. The reservosome matrix contains planar membranes, vesicles and lipid inclusions. Some of the latter may assume rectangular or sword-shaped crystalloid forms surrounded by a phospholipid monolayer, resembling the cholesterol crystals in foam cells. Methodology/Principal Findings: Using Nile Red fluorimetry and fluorescence microscopy, as well as electron microscopy, we have established a direct correlation between serum concentration in culture medium and the presence of crystalloid lipid inclusions. Starting from a reservosome purified fraction, we have developed a fractionation protocol to isolate lipid inclusions. Gas-chromatography mass-spectrometry (GC-MS) analysis revealed that lipid inclusions are composed mainly by cholesterol and cholesterol esters. Moreover, when the parasites with crystalloid lipid-loaded reservosomes were maintained in serum free medium for 48 hours the inclusions disappeared almost completely, including the sword shaped ones. Conclusions/Significance: Taken together, our results suggest that epimastigote forms of T. cruzi store high amounts of neutral lipids from extracellular medium, mostly cholesterol or cholesterol esters inside reservosomes. Interestingly, the parasites are able to disassemble the reservosome cholesterol crystalloid inclusions when submitted to serum starvation. |
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info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleTrypanosoma cruzi epimastigotes are able to store and mobilize high amounts of cholesterol in reservosome lipid inclusions20112012-06-27T18:22:08Z2012-06-27T18:22:08ZBackground: Reservosomes are lysosome-related organelles found in Trypanosoma cruzi epimastigotes. They represent the last step in epimastigote endocytic route, accumulating a set of proteins and enzymes related to protein digestion and lipid metabolism. The reservosome matrix contains planar membranes, vesicles and lipid inclusions. Some of the latter may assume rectangular or sword-shaped crystalloid forms surrounded by a phospholipid monolayer, resembling the cholesterol crystals in foam cells. Methodology/Principal Findings: Using Nile Red fluorimetry and fluorescence microscopy, as well as electron microscopy, we have established a direct correlation between serum concentration in culture medium and the presence of crystalloid lipid inclusions. Starting from a reservosome purified fraction, we have developed a fractionation protocol to isolate lipid inclusions. Gas-chromatography mass-spectrometry (GC-MS) analysis revealed that lipid inclusions are composed mainly by cholesterol and cholesterol esters. Moreover, when the parasites with crystalloid lipid-loaded reservosomes were maintained in serum free medium for 48 hours the inclusions disappeared almost completely, including the sword shaped ones. Conclusions/Significance: Taken together, our results suggest that epimastigote forms of T. cruzi store high amounts of neutral lipids from extracellular medium, mostly cholesterol or cholesterol esters inside reservosomes. Interestingly, the parasites are able to disassemble the reservosome cholesterol crystalloid inclusions when submitted to serum starvation.12 p. : il.Submitted by Lívia Oliveira (livia.oliveira2@hotmail.com) on 2012-06-27T15:00:10Z No. of bitstreams: 1 Plos One,v.6,n.7,p.e22359,2011.pdf: 2088336 bytes, checksum: b90db6de1fd976cffa4d1e7d64f73793 (MD5)Approved for entry into archive by Regina Mello(mrmello@inmetro.gov.br) on 2012-06-27T17:23:26Z (GMT) No. of bitstreams: 1 Plos One,v.6,n.7,p.e22359,2011.pdf: 2088336 bytes, checksum: b90db6de1fd976cffa4d1e7d64f73793 (MD5)Made available in DSpace on 2012-06-27T18:22:08Z (GMT). No. of bitstreams: 1 Plos One,v.6,n.7,p.e22359,2011.pdf: 2088336 bytes, checksum: b90db6de1fd976cffa4d1e7d64f73793 (MD5) Previous issue date: 2011enghttp://hdl.handle.net/10926/1738DMD_hdl_10926/1738PEREIRA, Miria G. et al. Trypanosoma cruzi epimastigotes are able to store and mobilize high amounts of cholesterol in reservosome lipid inclusions. Plos One, v. 6, n.7, 2011.Pereira, Miria GomesNakayasu, Ernesto SatoshiSant’Anna, CelsoCicco, Nuccia Nicole Theodoro deAtella, Geórgia CorrêaSouza, Wanderley deAlmeida, Igor Correia deSilva, Narcisa Leal da Cunha einfo:eu-repo/semantics/openAccessreponame:Repositório Institucional do INMETROinstname:Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO)instacron:INMETROPereira_2011.pdfhttp://xrepo01s.inmetro.gov.br/bitstream/10926/1738/7/Pereira_2011.pdfapplication/pdf2088336http://xrepo01s.inmetro.gov.br/bitstream/10926/1738/7/Pereira_2011.pdfb90db6de1fd976cffa4d1e7d64f73793MD510926_1738_7Plos%20One%2cv.6%2cn.7%2cp.e22359%2c2011.pdf.txthttp://xrepo01s.inmetro.gov.br/bitstream/10926/1738/6/Plos%20One%2cv.6%2cn.7%2cp.e22359%2c2011.pdf.txttext/plain55308http://xrepo01s.inmetro.gov.br/bitstream/10926/1738/6/Plos%20One%2cv.6%2cn.7%2cp.e22359%2c2011.pdf.txt8ccb3e52c2554167d5d9e0f195035bd1MD510926_1738_6Pereira_2011.pdf.txthttp://xrepo01s.inmetro.gov.br/bitstream/10926/1738/8/Pereira_2011.pdf.txttext/plain55308http://xrepo01s.inmetro.gov.br/bitstream/10926/1738/8/Pereira_2011.pdf.txt8ccb3e52c2554167d5d9e0f195035bd1MD510926_1738_82024-04-22T15:43:07Zoai:xrepo01s.inmetro.gov.br:10926/1738Repositório de Publicaçõeshttp://repositorios.inmetro.gov.br/oai/requestopendoar:2012-10-22T12:09:16Repositório Institucional do INMETRO - Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO)false |
dc.title.none.fl_str_mv |
Trypanosoma cruzi epimastigotes are able to store and mobilize high amounts of cholesterol in reservosome lipid inclusions |
title |
Trypanosoma cruzi epimastigotes are able to store and mobilize high amounts of cholesterol in reservosome lipid inclusions |
spellingShingle |
Trypanosoma cruzi epimastigotes are able to store and mobilize high amounts of cholesterol in reservosome lipid inclusions Pereira, Miria Gomes |
title_short |
Trypanosoma cruzi epimastigotes are able to store and mobilize high amounts of cholesterol in reservosome lipid inclusions |
title_full |
Trypanosoma cruzi epimastigotes are able to store and mobilize high amounts of cholesterol in reservosome lipid inclusions |
title_fullStr |
Trypanosoma cruzi epimastigotes are able to store and mobilize high amounts of cholesterol in reservosome lipid inclusions |
title_full_unstemmed |
Trypanosoma cruzi epimastigotes are able to store and mobilize high amounts of cholesterol in reservosome lipid inclusions |
title_sort |
Trypanosoma cruzi epimastigotes are able to store and mobilize high amounts of cholesterol in reservosome lipid inclusions |
author |
Pereira, Miria Gomes |
author_facet |
Pereira, Miria Gomes Nakayasu, Ernesto Satoshi Sant’Anna, Celso Cicco, Nuccia Nicole Theodoro de Atella, Geórgia Corrêa Souza, Wanderley de Almeida, Igor Correia de Silva, Narcisa Leal da Cunha e |
author_role |
author |
author2 |
Nakayasu, Ernesto Satoshi Sant’Anna, Celso Cicco, Nuccia Nicole Theodoro de Atella, Geórgia Corrêa Souza, Wanderley de Almeida, Igor Correia de Silva, Narcisa Leal da Cunha e |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Pereira, Miria Gomes Nakayasu, Ernesto Satoshi Sant’Anna, Celso Cicco, Nuccia Nicole Theodoro de Atella, Geórgia Corrêa Souza, Wanderley de Almeida, Igor Correia de Silva, Narcisa Leal da Cunha e |
description |
Background: Reservosomes are lysosome-related organelles found in Trypanosoma cruzi epimastigotes. They represent the last step in epimastigote endocytic route, accumulating a set of proteins and enzymes related to protein digestion and lipid metabolism. The reservosome matrix contains planar membranes, vesicles and lipid inclusions. Some of the latter may assume rectangular or sword-shaped crystalloid forms surrounded by a phospholipid monolayer, resembling the cholesterol crystals in foam cells. Methodology/Principal Findings: Using Nile Red fluorimetry and fluorescence microscopy, as well as electron microscopy, we have established a direct correlation between serum concentration in culture medium and the presence of crystalloid lipid inclusions. Starting from a reservosome purified fraction, we have developed a fractionation protocol to isolate lipid inclusions. Gas-chromatography mass-spectrometry (GC-MS) analysis revealed that lipid inclusions are composed mainly by cholesterol and cholesterol esters. Moreover, when the parasites with crystalloid lipid-loaded reservosomes were maintained in serum free medium for 48 hours the inclusions disappeared almost completely, including the sword shaped ones. Conclusions/Significance: Taken together, our results suggest that epimastigote forms of T. cruzi store high amounts of neutral lipids from extracellular medium, mostly cholesterol or cholesterol esters inside reservosomes. Interestingly, the parasites are able to disassemble the reservosome cholesterol crystalloid inclusions when submitted to serum starvation. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011 |
dc.date.available.fl_str_mv |
2012-06-27T18:22:08Z |
dc.date.accessioned.fl_str_mv |
2012-06-27T18:22:08Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10926/1738 DMD_hdl_10926/1738 |
dc.identifier.citation.fl_str_mv |
PEREIRA, Miria G. et al. Trypanosoma cruzi epimastigotes are able to store and mobilize high amounts of cholesterol in reservosome lipid inclusions. Plos One, v. 6, n.7, 2011. |
url |
http://hdl.handle.net/10926/1738 |
identifier_str_mv |
DMD_hdl_10926/1738 PEREIRA, Miria G. et al. Trypanosoma cruzi epimastigotes are able to store and mobilize high amounts of cholesterol in reservosome lipid inclusions. Plos One, v. 6, n.7, 2011. |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.bitstream.fl_str_mv |
application/pdf text/plain text/plain |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional do INMETRO instname:Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO) instacron:INMETRO |
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Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO) |
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INMETRO |
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INMETRO |
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Repositório Institucional do INMETRO |
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Repositório Institucional do INMETRO |
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Repositório Institucional do INMETRO - Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO) |
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