Early and late pathogenic events of newborn mice encephalitis experimentally induced by itacaiunas and curionoópolis bracorhabdoviruses infection
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional do INMETRO |
Texto Completo: | http://hdl.handle.net/10926/1883 |
Resumo: | In previous reports we proposed a new genus for Rhabdoviridae and described neurotropic preference and gross neuropathology in newborn albino Swiss mice after Curionopolis and Itacaiunas infections. In the present report a timecourse study of experimental encephalitis induced by Itacaiunas and Curionopolis virus was conducted both in vivo and in vitro to investigate cellular targets and the sequence of neuroinvasion. We also investigate, after intranasal inoculation, clinical signs, histopathology and apoptosis in correlation with viral immunolabeling at different time points. Curionopolis and Itacaiunas viral antigens were first detected in the parenchyma of olfactory pathways at 2 and 3 days post-inoculation (dpi) and the first clinical signs were observed at 4 and 8 dpi, respectively. After Curionopolis infection, the mortality rate was 100% between 5 and 6 dpi, and 35% between 8 and 15 dpi after Itacaiunas infection. We identified CNS mice cell types both in vivo and in vitro and the temporal sequence of neuroanatomical olfactory areas infected by Itacaiunas and Curionopolis virus. Distinct virulences were reflected in the neuropathological changes including TUNEL immunolabeling and cytopathic effects, more intense and precocious after intracerebral or in vitro inoculations of Curionopolis than after Itacaiunas virus. In vitro studies revealed neuronal but not astrocyte or microglial cytopathic effects at 2 dpi, with monolayer destruction occurring at 5 and 7 dpi with Curionopolis and Itacaiunas virus, respectively. Ultrastructural changes included virus budding associated with interstitial and perivascular edema, endothelial hypertrophy, a reduced and/or collapsed small vessel luminal area, thickening of the capillary basement membrane, and presence of phagocytosed apoptotic bodies. Glial cells with viral budding similar to oligodendrocytes were infected with Itacaiunas virus but not with Curionopolis virus. Thus, Curionopolis and Itacaiunas viruses share many pathological and clinical features present in other rhabdoviruses but distinct virulence and glial targets in newborn albino Swiss mice brain. |
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info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleEarly and late pathogenic events of newborn mice encephalitis experimentally induced by itacaiunas and curionoópolis bracorhabdoviruses infection20092012-08-13T14:41:48Z2012-08-13T14:41:48ZIn previous reports we proposed a new genus for Rhabdoviridae and described neurotropic preference and gross neuropathology in newborn albino Swiss mice after Curionopolis and Itacaiunas infections. In the present report a timecourse study of experimental encephalitis induced by Itacaiunas and Curionopolis virus was conducted both in vivo and in vitro to investigate cellular targets and the sequence of neuroinvasion. We also investigate, after intranasal inoculation, clinical signs, histopathology and apoptosis in correlation with viral immunolabeling at different time points. Curionopolis and Itacaiunas viral antigens were first detected in the parenchyma of olfactory pathways at 2 and 3 days post-inoculation (dpi) and the first clinical signs were observed at 4 and 8 dpi, respectively. After Curionopolis infection, the mortality rate was 100% between 5 and 6 dpi, and 35% between 8 and 15 dpi after Itacaiunas infection. We identified CNS mice cell types both in vivo and in vitro and the temporal sequence of neuroanatomical olfactory areas infected by Itacaiunas and Curionopolis virus. Distinct virulences were reflected in the neuropathological changes including TUNEL immunolabeling and cytopathic effects, more intense and precocious after intracerebral or in vitro inoculations of Curionopolis than after Itacaiunas virus. In vitro studies revealed neuronal but not astrocyte or microglial cytopathic effects at 2 dpi, with monolayer destruction occurring at 5 and 7 dpi with Curionopolis and Itacaiunas virus, respectively. Ultrastructural changes included virus budding associated with interstitial and perivascular edema, endothelial hypertrophy, a reduced and/or collapsed small vessel luminal area, thickening of the capillary basement membrane, and presence of phagocytosed apoptotic bodies. Glial cells with viral budding similar to oligodendrocytes were infected with Itacaiunas virus but not with Curionopolis virus. Thus, Curionopolis and Itacaiunas viruses share many pathological and clinical features present in other rhabdoviruses but distinct virulence and glial targets in newborn albino Swiss mice brain.12 p. : il.Submitted by Lívia Oliveira (livia.oliveira2@hotmail.com) on 2012-02-29T12:18:03Z No. of bitstreams: 1 Souza6_2008.pdf: 1586955 bytes, checksum: 08d779edba3b00ed4b76b2da28b5510b (MD5)Rejected by Catarina Soares(cfsoares@inmetro.gov.br), reason: Corrigir nomes dos autores na referencia bibliografica on 2012-03-15T18:14:42Z (GMT)Submitted by Lívia Oliveira (livia.oliveira2@hotmail.com) on 2012-03-16T14:06:18Z No. of bitstreams: 1 Souza6_2008.pdf: 1586955 bytes, checksum: 08d779edba3b00ed4b76b2da28b5510b (MD5)Approved for entry into archive by Regina Mello(mrmello@inmetro.gov.br) on 2012-07-02T12:47:12Z (GMT) No. of bitstreams: 1 Souza6_2008.pdf: 1586955 bytes, checksum: 08d779edba3b00ed4b76b2da28b5510b (MD5)Made available in DSpace on 2012-08-13T14:41:48Z (GMT). No. of bitstreams: 1 Souza6_2008.pdf: 1586955 bytes, checksum: 08d779edba3b00ed4b76b2da28b5510b (MD5) Previous issue date: 2009enghttp://hdl.handle.net/10926/1883DMD_hdl_10926/1883DINIZ, José Antonio Picanço et al. Early and late pathogenic events of newborn mice encephalitis experimentally induced by itacaiunas and curionoópolis bracorhabdoviruses infection. Plos One, v. 3, 2008.Diniz, José Antonio PicançoSantos, Zaire Alves dosBraga, Marcio Augusto GalvãoDias, Ádila Liliane BarrosSilva, Daisy Elaine Andrade daMedeiros, Daniele Barbosa de AlmeidaBarros, Vera Lucia Reis de SouzaChiang, Jannifer OliveiraZoghbi, Kendra Eyllen de FreitasQuaresma, Juarez Antônio SimõesTakiya, Christina MaedaMoura Neto, VivaldoSouza, Wanderley deVasconcelos, Pedro Fernando da CostaDiniz, Cristovam Wanderley Picançoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional do INMETROinstname:Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO)instacron:INMETRODiniz_2008.pdfhttp://xrepo01s.inmetro.gov.br/bitstream/10926/1883/1/Diniz_2008.pdfapplication/pdf1586955http://xrepo01s.inmetro.gov.br/bitstream/10926/1883/1/Diniz_2008.pdf08d779edba3b00ed4b76b2da28b5510bMD510926_1883_1Diniz_2008.pdf.txthttp://xrepo01s.inmetro.gov.br/bitstream/10926/1883/6/Diniz_2008.pdf.txttext/plain58221http://xrepo01s.inmetro.gov.br/bitstream/10926/1883/6/Diniz_2008.pdf.txta699ccefc50c4ae3f8b2d7fc701b8813MD510926_1883_62024-04-22T15:43:02Zoai:xrepo01s.inmetro.gov.br:10926/1883Repositório de Publicaçõeshttp://repositorios.inmetro.gov.br/oai/requestopendoar:2012-11-28T16:42:34Repositório Institucional do INMETRO - Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO)false |
dc.title.none.fl_str_mv |
Early and late pathogenic events of newborn mice encephalitis experimentally induced by itacaiunas and curionoópolis bracorhabdoviruses infection |
title |
Early and late pathogenic events of newborn mice encephalitis experimentally induced by itacaiunas and curionoópolis bracorhabdoviruses infection |
spellingShingle |
Early and late pathogenic events of newborn mice encephalitis experimentally induced by itacaiunas and curionoópolis bracorhabdoviruses infection Diniz, José Antonio Picanço |
title_short |
Early and late pathogenic events of newborn mice encephalitis experimentally induced by itacaiunas and curionoópolis bracorhabdoviruses infection |
title_full |
Early and late pathogenic events of newborn mice encephalitis experimentally induced by itacaiunas and curionoópolis bracorhabdoviruses infection |
title_fullStr |
Early and late pathogenic events of newborn mice encephalitis experimentally induced by itacaiunas and curionoópolis bracorhabdoviruses infection |
title_full_unstemmed |
Early and late pathogenic events of newborn mice encephalitis experimentally induced by itacaiunas and curionoópolis bracorhabdoviruses infection |
title_sort |
Early and late pathogenic events of newborn mice encephalitis experimentally induced by itacaiunas and curionoópolis bracorhabdoviruses infection |
author |
Diniz, José Antonio Picanço |
author_facet |
Diniz, José Antonio Picanço Santos, Zaire Alves dos Braga, Marcio Augusto Galvão Dias, Ádila Liliane Barros Silva, Daisy Elaine Andrade da Medeiros, Daniele Barbosa de Almeida Barros, Vera Lucia Reis de Souza Chiang, Jannifer Oliveira Zoghbi, Kendra Eyllen de Freitas Quaresma, Juarez Antônio Simões Takiya, Christina Maeda Moura Neto, Vivaldo Souza, Wanderley de Vasconcelos, Pedro Fernando da Costa Diniz, Cristovam Wanderley Picanço |
author_role |
author |
author2 |
Santos, Zaire Alves dos Braga, Marcio Augusto Galvão Dias, Ádila Liliane Barros Silva, Daisy Elaine Andrade da Medeiros, Daniele Barbosa de Almeida Barros, Vera Lucia Reis de Souza Chiang, Jannifer Oliveira Zoghbi, Kendra Eyllen de Freitas Quaresma, Juarez Antônio Simões Takiya, Christina Maeda Moura Neto, Vivaldo Souza, Wanderley de Vasconcelos, Pedro Fernando da Costa Diniz, Cristovam Wanderley Picanço |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Diniz, José Antonio Picanço Santos, Zaire Alves dos Braga, Marcio Augusto Galvão Dias, Ádila Liliane Barros Silva, Daisy Elaine Andrade da Medeiros, Daniele Barbosa de Almeida Barros, Vera Lucia Reis de Souza Chiang, Jannifer Oliveira Zoghbi, Kendra Eyllen de Freitas Quaresma, Juarez Antônio Simões Takiya, Christina Maeda Moura Neto, Vivaldo Souza, Wanderley de Vasconcelos, Pedro Fernando da Costa Diniz, Cristovam Wanderley Picanço |
description |
In previous reports we proposed a new genus for Rhabdoviridae and described neurotropic preference and gross neuropathology in newborn albino Swiss mice after Curionopolis and Itacaiunas infections. In the present report a timecourse study of experimental encephalitis induced by Itacaiunas and Curionopolis virus was conducted both in vivo and in vitro to investigate cellular targets and the sequence of neuroinvasion. We also investigate, after intranasal inoculation, clinical signs, histopathology and apoptosis in correlation with viral immunolabeling at different time points. Curionopolis and Itacaiunas viral antigens were first detected in the parenchyma of olfactory pathways at 2 and 3 days post-inoculation (dpi) and the first clinical signs were observed at 4 and 8 dpi, respectively. After Curionopolis infection, the mortality rate was 100% between 5 and 6 dpi, and 35% between 8 and 15 dpi after Itacaiunas infection. We identified CNS mice cell types both in vivo and in vitro and the temporal sequence of neuroanatomical olfactory areas infected by Itacaiunas and Curionopolis virus. Distinct virulences were reflected in the neuropathological changes including TUNEL immunolabeling and cytopathic effects, more intense and precocious after intracerebral or in vitro inoculations of Curionopolis than after Itacaiunas virus. In vitro studies revealed neuronal but not astrocyte or microglial cytopathic effects at 2 dpi, with monolayer destruction occurring at 5 and 7 dpi with Curionopolis and Itacaiunas virus, respectively. Ultrastructural changes included virus budding associated with interstitial and perivascular edema, endothelial hypertrophy, a reduced and/or collapsed small vessel luminal area, thickening of the capillary basement membrane, and presence of phagocytosed apoptotic bodies. Glial cells with viral budding similar to oligodendrocytes were infected with Itacaiunas virus but not with Curionopolis virus. Thus, Curionopolis and Itacaiunas viruses share many pathological and clinical features present in other rhabdoviruses but distinct virulence and glial targets in newborn albino Swiss mice brain. |
publishDate |
2009 |
dc.date.issued.fl_str_mv |
2009 |
dc.date.available.fl_str_mv |
2012-08-13T14:41:48Z |
dc.date.accessioned.fl_str_mv |
2012-08-13T14:41:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10926/1883 DMD_hdl_10926/1883 |
dc.identifier.citation.fl_str_mv |
DINIZ, José Antonio Picanço et al. Early and late pathogenic events of newborn mice encephalitis experimentally induced by itacaiunas and curionoópolis bracorhabdoviruses infection. Plos One, v. 3, 2008. |
url |
http://hdl.handle.net/10926/1883 |
identifier_str_mv |
DMD_hdl_10926/1883 DINIZ, José Antonio Picanço et al. Early and late pathogenic events of newborn mice encephalitis experimentally induced by itacaiunas and curionoópolis bracorhabdoviruses infection. Plos One, v. 3, 2008. |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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openAccess |
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application/pdf text/plain |
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Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO) |
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INMETRO |
institution |
INMETRO |
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Repositório Institucional do INMETRO |
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Repositório Institucional do INMETRO |
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Repositório Institucional do INMETRO - Instituto Nacional de Metrologia, Qualidade e Tecnologia (INMETRO) |
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