Effect of isodillapiole on the expression of the insecticide resistance genes GSTE7 and CYP6N12 in Aedes aegypti from central Amazonia

• Autor(a) principal: Lima, V. S.
• Data de Publicação: 2015
• Outros Autores: Pinto, A. C., Rafael, Míriam Silva
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional do INPA
• Texto Completo: https://repositorio.inpa.gov.br/handle/1/14982
• topic: Insecticide; Isodillapiole; Unclassified Drug; 1,3 Dioxolane Derivative; Cytochrome P450; Dill Apiole; Glutathione Transferase; Insect Proteins; Adult; Aedes Aegypti; Amazona; Animals Experiment; Arthropod Larva; Pest Control, Biological; Concentration (parameters); Controlled Study; Cyp6n12 Gene; Down Regulation; Female; Gene; Gene Expression Regulation; Gste7 Gene; Insect Resistance; Insecticide Resistance; Male; Metabolic Stress; Nonhuman; Reverse Transcription Polymerase Chain Reaction; Toxicity Testing; Up-regulation; Aedes; Animals; Drug Effects; Genetics; Metabolism; Aedes; Animal; Cytochrome P-450 Enzyme System; Dioxoles; Glutathione Transferase; Insect Proteins; Insecticide Resistance

The yellow fever mosquito Aedes (Stegomyia) aegypti is the main vector of dengue arbovirus and other arboviruses. Dengue prevention measures for the control of A. aegypti involve mainly the use of synthetic insecticides. The constant use of insecticides has caused resistance in this mosquito. Alternative studies on plant extracts and their products have been conducted with the aim of controlling the spread of the mosquito. Dillapiole is a compound found in essential oils of the plant Piper aduncum (Piperaceae) which has been effective as a biopesticide against A. aegypti. Isodillapiole is a semisynthetic substance obtained by the isomerization of dillapiole. In the present study, isodillapiole was evaluated for its potential to induce differential expression of insecticide resistance genes (GSTE7 and CYP6N12) in 3rd instar larvae of A. aegypti. These larvae were exposed to this compound at two concentrations (20 and 40 μg/mL) for 4 h during four generations (G1, G2, G3, and G4). Quantitative RT-PCR was used to assess the expression of GSTE7 and CYP6N12 genes. GSTE7 and CYP6N12 relative expression levels were higher at 20 than at 40 μg/mL and varied among generations. The decrease in GSTE7 and CYP6N12 expression levels at the highest isodillapiole concentration suggests that larvae may have suffered from metabolic stress, revealing a potential alternative product in the control of A. aegypti. © FUNPEC-RP.