Phlebotomine sandfly (Diptera: Psychodidae) diversity and their leishmania DNA in a hot spot of American cutaneous leishmaniasis human cases along the Brazilian border with Peru and Bolivia

• Autor(a) principal: Teles, Carolina Bioni Garcia
• Data de Publicação: 2016
• Outros Autores: dos Santos, Ana Paula Azevedo, Freitas, Rui Alves de, Oliveira, Arley Faria José de, Ogawa, Guilherme Maerschner, Rodrigues, Moreno S., Pessoa, Felipe Arley Costa, Medeiros, Jansen Fernandes de, Camargo, Luis Marcelo Aranha
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional do INPA
• Texto Completo: https://repositorio.inpa.gov.br/handle/1/14519
• topic: Dna; Dna, Kinetoplast; Animals; Biodiversity; Bolivia; Brasil; Classification; Female; Genetics; Genotype; Human; Insect Vector; Leishmania; Parasitology; Peru; Polymerase Chain Reaction; Population Density; Psychodidae; Skin Leishmaniasis; Transmission; Animal; Biodiversity; Bolivia; Brasil; Dna; Dna, Kinetoplast; Female; Genotype; Humans; Insect Vectors; Leishmania; Leishmaniasis, Cutaneous; Peru; Polymerase Chain Reaction; Population Density; Psychodidae

In this study, we identified the phlebotomine sandfly vectors involved in the transmission of American Cutaneous Leishmaniasis (ACL) in Assis Brasil, Acre, Brazil, which is located on the Brazil-Peru-Bolivia frontier. The genotyping of Leishmania in phlebotomines was performed using polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism. A total of 6,850 sandflies comprising 67 species were captured by using CDC light traps in rural areas of the municipality. Three sandfly species were found in the state of Acre for the first time: Lutzomyia georgii, Lu. complexa and Lu. evangelistai. The predominant species was Lu. auraensis/Lu. ruifreitasi and Lu. davisi (total 59.27%). 32 of 368 pools were positive for the presence of Leishmania DNA (16 pools corresponding to Lu. davisi, and 16 corresponding to Lu. auraensis/Lu. ruifreitasi), with a minimal infection prevalence of 1.85% in Lu. davisi and 2.05% in Lu. auraensis/Lu. ruifreitasi. The Leishmania species found showed maximum identity with L. (Viannia) guyanensis and L. (V.) braziliensis in both phlebotomine species. Based on these results and similar scenarios previously described along the Brazil/Peru/Bolivia tri-border, the studied area must take into consideration the possibility of Lu. davisi and Lu. auraensis/Lu. ruifreitasi as probable vectors of ACL in this municipality. © 2016, Fundacao Oswaldo Cruz. All rights reserved.