Identification of a fibrinogen-related protein (FBN9) gene in neotropical anopheline mosquitoes
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional do INPA |
Texto Completo: | https://repositorio.inpa.gov.br/handle/1/16211 |
Resumo: | Background: Malaria has a devastating impact on worldwide public health in many tropical areas. Studies on vector immunity are important for the overall understanding of the parasite-vector interaction and for the design of novel strategies to control malaria. A member of the fibrinogen-related protein family, fbn9, has been well studied in Anopheles gambiae and has been shown to be an important component of the mosquito immune system. However, little is known about this gene in neotropical anopheline species. Methods. This article describes the identification and characterization of the fbn9 gene partial sequences from four species of neotropical anopheline primary and secondary vectors: Anopheles darlingi, Anopheles nuneztovari, Anopheles aquasalis, and Anopheles albitarsis (namely Anopheles marajoara). Degenerate primers were designed based on comparative analysis of publicly available Aedes aegypti and An. gambiae gene sequences and used to clone putative homologs in the neotropical species. Sequence comparisons and Bayesian phylogenetic analyses were then performed to better understand the molecular diversity of this gene in evolutionary distant anopheline species, belonging to different subgenera. Results: Comparisons of the fbn9 gene sequences of the neotropical anophelines and their homologs in the An. gambiae complex (Gambiae complex) showed high conservation at the nucleotide and amino acid levels, although some sites show significant differentiation (non-synonymous substitutions). Furthermore, phylogenetic analysis of fbn9 nucleotide sequences showed that neotropical anophelines and African mosquitoes form two well-supported clades, mirroring their separation into two different subgenera. Conclusions: The present work adds new insights into the conserved role of fbn9 in insect immunity in a broader range of anopheline species and reinforces the possibility of manipulating mosquito immunity to design novel pathogen control strategies. © 2011 Oliveira et al; licensee BioMed Central Ltd. |
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Oliveira, Sabrina Alberti Nóbrega deIbraim, Izabela CoimbraTadei, Wanderli PedroRuiz, Jerônimo ConceiçãoNahum, Laila AlvesBrito, Cristiana Ferreira Alves deMoreira, Luciano Andrade2020-05-31T18:24:28Z2020-05-31T18:24:28Z2011https://repositorio.inpa.gov.br/handle/1/1621110.1186/1475-2875-10-21Background: Malaria has a devastating impact on worldwide public health in many tropical areas. Studies on vector immunity are important for the overall understanding of the parasite-vector interaction and for the design of novel strategies to control malaria. A member of the fibrinogen-related protein family, fbn9, has been well studied in Anopheles gambiae and has been shown to be an important component of the mosquito immune system. However, little is known about this gene in neotropical anopheline species. Methods. This article describes the identification and characterization of the fbn9 gene partial sequences from four species of neotropical anopheline primary and secondary vectors: Anopheles darlingi, Anopheles nuneztovari, Anopheles aquasalis, and Anopheles albitarsis (namely Anopheles marajoara). Degenerate primers were designed based on comparative analysis of publicly available Aedes aegypti and An. gambiae gene sequences and used to clone putative homologs in the neotropical species. Sequence comparisons and Bayesian phylogenetic analyses were then performed to better understand the molecular diversity of this gene in evolutionary distant anopheline species, belonging to different subgenera. Results: Comparisons of the fbn9 gene sequences of the neotropical anophelines and their homologs in the An. gambiae complex (Gambiae complex) showed high conservation at the nucleotide and amino acid levels, although some sites show significant differentiation (non-synonymous substitutions). Furthermore, phylogenetic analysis of fbn9 nucleotide sequences showed that neotropical anophelines and African mosquitoes form two well-supported clades, mirroring their separation into two different subgenera. Conclusions: The present work adds new insights into the conserved role of fbn9 in insect immunity in a broader range of anopheline species and reinforces the possibility of manipulating mosquito immunity to design novel pathogen control strategies. © 2011 Oliveira et al; licensee BioMed Central Ltd.Volume 10Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessAmino AcidFibrinogen Related Protein 9Insect ProteinsNucleotideUnclassified DrugFibrinogenImmunoglobulinAedes AegyptiAmino Acid SubstitutionAnophelesAnopheles AlbitarsisAnopheles AquasalisAnopheles DarlingiAnopheles MarajoaraAnopheles MerusAnopheles NuneztovariAnopheles QuadriannulatusBayes TheoremCladisticsComparative StudyControlled StudyDisease CarrierEvolutionary HomologyGene IdentificationGene SequenceGenetic VariabilityGenotypeCloning, MolecularNeotropicsNonhumanNucleotide SequencePhylogenySequence HomologyPolymorphism, Single NucleotideAmino Acid SequenceAnimalsBrasilClassificationGeneGeneticsImmunologyMalariaEvolution, MolecularParasitologySequence AnalysisAmino Acid SequenceAnimalAnophelesBase SequenceBrasilCloning, MolecularEvolution, MolecularFibrinogenGenes, InsectImmunoglobulinsInsect VectorsMalariaPhylogenySequence AnalysisIdentification of a fibrinogen-related protein (FBN9) gene in neotropical anopheline mosquitoesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMalaria Journalengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALIdentification.pdfIdentification.pdfapplication/pdf1828867https://repositorio.inpa.gov.br/bitstream/1/16211/1/Identification.pdf56f697c019ca1f3b42db7c04c1eb6159MD511/162112020-05-31 14:58:32.674oai:repositorio:1/16211Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-05-31T18:58:32Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false |
dc.title.en.fl_str_mv |
Identification of a fibrinogen-related protein (FBN9) gene in neotropical anopheline mosquitoes |
title |
Identification of a fibrinogen-related protein (FBN9) gene in neotropical anopheline mosquitoes |
spellingShingle |
Identification of a fibrinogen-related protein (FBN9) gene in neotropical anopheline mosquitoes Oliveira, Sabrina Alberti Nóbrega de Amino Acid Fibrinogen Related Protein 9 Insect Proteins Nucleotide Unclassified Drug Fibrinogen Immunoglobulin Aedes Aegypti Amino Acid Substitution Anopheles Anopheles Albitarsis Anopheles Aquasalis Anopheles Darlingi Anopheles Marajoara Anopheles Merus Anopheles Nuneztovari Anopheles Quadriannulatus Bayes Theorem Cladistics Comparative Study Controlled Study Disease Carrier Evolutionary Homology Gene Identification Gene Sequence Genetic Variability Genotype Cloning, Molecular Neotropics Nonhuman Nucleotide Sequence Phylogeny Sequence Homology Polymorphism, Single Nucleotide Amino Acid Sequence Animals Brasil Classification Gene Genetics Immunology Malaria Evolution, Molecular Parasitology Sequence Analysis Amino Acid Sequence Animal Anopheles Base Sequence Brasil Cloning, Molecular Evolution, Molecular Fibrinogen Genes, Insect Immunoglobulins Insect Vectors Malaria Phylogeny Sequence Analysis |
title_short |
Identification of a fibrinogen-related protein (FBN9) gene in neotropical anopheline mosquitoes |
title_full |
Identification of a fibrinogen-related protein (FBN9) gene in neotropical anopheline mosquitoes |
title_fullStr |
Identification of a fibrinogen-related protein (FBN9) gene in neotropical anopheline mosquitoes |
title_full_unstemmed |
Identification of a fibrinogen-related protein (FBN9) gene in neotropical anopheline mosquitoes |
title_sort |
Identification of a fibrinogen-related protein (FBN9) gene in neotropical anopheline mosquitoes |
author |
Oliveira, Sabrina Alberti Nóbrega de |
author_facet |
Oliveira, Sabrina Alberti Nóbrega de Ibraim, Izabela Coimbra Tadei, Wanderli Pedro Ruiz, Jerônimo Conceição Nahum, Laila Alves Brito, Cristiana Ferreira Alves de Moreira, Luciano Andrade |
author_role |
author |
author2 |
Ibraim, Izabela Coimbra Tadei, Wanderli Pedro Ruiz, Jerônimo Conceição Nahum, Laila Alves Brito, Cristiana Ferreira Alves de Moreira, Luciano Andrade |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira, Sabrina Alberti Nóbrega de Ibraim, Izabela Coimbra Tadei, Wanderli Pedro Ruiz, Jerônimo Conceição Nahum, Laila Alves Brito, Cristiana Ferreira Alves de Moreira, Luciano Andrade |
dc.subject.eng.fl_str_mv |
Amino Acid Fibrinogen Related Protein 9 Insect Proteins Nucleotide Unclassified Drug Fibrinogen Immunoglobulin Aedes Aegypti Amino Acid Substitution Anopheles Anopheles Albitarsis Anopheles Aquasalis Anopheles Darlingi Anopheles Marajoara Anopheles Merus Anopheles Nuneztovari Anopheles Quadriannulatus Bayes Theorem Cladistics Comparative Study Controlled Study Disease Carrier Evolutionary Homology Gene Identification Gene Sequence Genetic Variability Genotype Cloning, Molecular Neotropics Nonhuman Nucleotide Sequence Phylogeny Sequence Homology Polymorphism, Single Nucleotide Amino Acid Sequence Animals Brasil Classification Gene Genetics Immunology Malaria Evolution, Molecular Parasitology Sequence Analysis Amino Acid Sequence Animal Anopheles Base Sequence Brasil Cloning, Molecular Evolution, Molecular Fibrinogen Genes, Insect Immunoglobulins Insect Vectors Malaria Phylogeny Sequence Analysis |
topic |
Amino Acid Fibrinogen Related Protein 9 Insect Proteins Nucleotide Unclassified Drug Fibrinogen Immunoglobulin Aedes Aegypti Amino Acid Substitution Anopheles Anopheles Albitarsis Anopheles Aquasalis Anopheles Darlingi Anopheles Marajoara Anopheles Merus Anopheles Nuneztovari Anopheles Quadriannulatus Bayes Theorem Cladistics Comparative Study Controlled Study Disease Carrier Evolutionary Homology Gene Identification Gene Sequence Genetic Variability Genotype Cloning, Molecular Neotropics Nonhuman Nucleotide Sequence Phylogeny Sequence Homology Polymorphism, Single Nucleotide Amino Acid Sequence Animals Brasil Classification Gene Genetics Immunology Malaria Evolution, Molecular Parasitology Sequence Analysis Amino Acid Sequence Animal Anopheles Base Sequence Brasil Cloning, Molecular Evolution, Molecular Fibrinogen Genes, Insect Immunoglobulins Insect Vectors Malaria Phylogeny Sequence Analysis |
description |
Background: Malaria has a devastating impact on worldwide public health in many tropical areas. Studies on vector immunity are important for the overall understanding of the parasite-vector interaction and for the design of novel strategies to control malaria. A member of the fibrinogen-related protein family, fbn9, has been well studied in Anopheles gambiae and has been shown to be an important component of the mosquito immune system. However, little is known about this gene in neotropical anopheline species. Methods. This article describes the identification and characterization of the fbn9 gene partial sequences from four species of neotropical anopheline primary and secondary vectors: Anopheles darlingi, Anopheles nuneztovari, Anopheles aquasalis, and Anopheles albitarsis (namely Anopheles marajoara). Degenerate primers were designed based on comparative analysis of publicly available Aedes aegypti and An. gambiae gene sequences and used to clone putative homologs in the neotropical species. Sequence comparisons and Bayesian phylogenetic analyses were then performed to better understand the molecular diversity of this gene in evolutionary distant anopheline species, belonging to different subgenera. Results: Comparisons of the fbn9 gene sequences of the neotropical anophelines and their homologs in the An. gambiae complex (Gambiae complex) showed high conservation at the nucleotide and amino acid levels, although some sites show significant differentiation (non-synonymous substitutions). Furthermore, phylogenetic analysis of fbn9 nucleotide sequences showed that neotropical anophelines and African mosquitoes form two well-supported clades, mirroring their separation into two different subgenera. Conclusions: The present work adds new insights into the conserved role of fbn9 in insect immunity in a broader range of anopheline species and reinforces the possibility of manipulating mosquito immunity to design novel pathogen control strategies. © 2011 Oliveira et al; licensee BioMed Central Ltd. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011 |
dc.date.accessioned.fl_str_mv |
2020-05-31T18:24:28Z |
dc.date.available.fl_str_mv |
2020-05-31T18:24:28Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.inpa.gov.br/handle/1/16211 |
dc.identifier.doi.none.fl_str_mv |
10.1186/1475-2875-10-21 |
url |
https://repositorio.inpa.gov.br/handle/1/16211 |
identifier_str_mv |
10.1186/1475-2875-10-21 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Volume 10 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Malaria Journal |
publisher.none.fl_str_mv |
Malaria Journal |
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INPA |
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