Ecological aspects and molecular detection of Leishmania DNA Ross (Kinetoplastida: Trypanosomatidae) in phlebotomine sandflies (Diptera: Psychodidae) in terra firme and várzea environments in the Middle Solimões Region, Amazonas State, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional do INPA |
Texto Completo: | https://repositorio.inpa.gov.br/handle/1/15923 |
Resumo: | Background: Phlebotomine sand flies (Diptera: Psychodidae) are insects of medical importance due to the role that some species play in the transmission of leishmaniasis. This work aimed to study some ecological aspects among sand flies fauna inhabiting two different environments: the várzea (lowland Amazonian forest) and terra firme (upland Amazonian forest), both located in Tefé Municipality, Amazonas State, Braziland to detect Leishmania infection in those phlebotomine populations. Methods: Sand flies were collected using HP light traps. Collection took place over the course of six months: January, February, April, August, September, and October of 2013. To detect natural infection by Leishmania, DNA samples were extracted from female sand flies and submitted to Polymerase Chain Reaction (PCR) targeting the kDNA gene; Leishmania species were identified by PCR-RFLP targeting the hsp70 gene and genetic sequencing. Results: In all, 5,716 individuals were collected, and 46 species were identified. Trichophoromyia ubiquitalis (3,330 - 58.26%) and Nyssomyia antunesi (661 - 11.26%) were the most abundant species. Species richness was greater in terra firme environments (42 species) than in the várzea environments (22 species), and forests ecotopes (43 species) were richer than peridomiciles (28 species). DNA of Leishmania was found in Th. ubiquitalis and Psychodopygus davisi, both of which inhabit the terra firme environment and sequencing analysis confirmed the presence of Leishmania (Viannia) lainsoni DNA in Th. ubiquitalis in Tefé Municipality. Conclusions: The high abundance of Th. ubiquitalis and Ps. davisi and detection of DNA of Leishmania sp. may indicate that both species could be putative vectors for American Cutaneous Leishmaniasis (ACL) in the terra firme environment of Tefé. The sand fly fauna found in várzea is rich and diverse, exhibiting several species, nevertheless the seasonal hydric stress during part of the year that could influence the local diversity, if compared with other studies. This is the first report in Amazonas State of Th. ubiquitalis with presence of L. (V.) lainsoni DNA. © 2015 Pereira et al.; licensee BioMed Central. |
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Pereira Júnior, Antonio MarquesTeles, Carolina Bioni GarciaAzevedo dos Santos, Ana Paula Silva deRodrigues, Moreno S.Marialva, Eric FabrícioPessoa, Felipe Arley CostaMedeiros, Jansen Fernandes de2020-05-20T14:15:56Z2020-05-20T14:15:56Z2015https://repositorio.inpa.gov.br/handle/1/1592310.1186/s13071-015-0789-2Background: Phlebotomine sand flies (Diptera: Psychodidae) are insects of medical importance due to the role that some species play in the transmission of leishmaniasis. This work aimed to study some ecological aspects among sand flies fauna inhabiting two different environments: the várzea (lowland Amazonian forest) and terra firme (upland Amazonian forest), both located in Tefé Municipality, Amazonas State, Braziland to detect Leishmania infection in those phlebotomine populations. Methods: Sand flies were collected using HP light traps. Collection took place over the course of six months: January, February, April, August, September, and October of 2013. To detect natural infection by Leishmania, DNA samples were extracted from female sand flies and submitted to Polymerase Chain Reaction (PCR) targeting the kDNA gene; Leishmania species were identified by PCR-RFLP targeting the hsp70 gene and genetic sequencing. Results: In all, 5,716 individuals were collected, and 46 species were identified. Trichophoromyia ubiquitalis (3,330 - 58.26%) and Nyssomyia antunesi (661 - 11.26%) were the most abundant species. Species richness was greater in terra firme environments (42 species) than in the várzea environments (22 species), and forests ecotopes (43 species) were richer than peridomiciles (28 species). DNA of Leishmania was found in Th. ubiquitalis and Psychodopygus davisi, both of which inhabit the terra firme environment and sequencing analysis confirmed the presence of Leishmania (Viannia) lainsoni DNA in Th. ubiquitalis in Tefé Municipality. Conclusions: The high abundance of Th. ubiquitalis and Ps. davisi and detection of DNA of Leishmania sp. may indicate that both species could be putative vectors for American Cutaneous Leishmaniasis (ACL) in the terra firme environment of Tefé. The sand fly fauna found in várzea is rich and diverse, exhibiting several species, nevertheless the seasonal hydric stress during part of the year that could influence the local diversity, if compared with other studies. This is the first report in Amazonas State of Th. ubiquitalis with presence of L. (V.) lainsoni DNA. © 2015 Pereira et al.; licensee BioMed Central.Volume 8, Número 1Attribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/openAccessDnaHeat Shock Protein 72Protozoal DnaBrasilDisease TransmissionDna ExtractionDna SequenceEcological Phenomena And FunctionsFemaleGene SequenceHumanKinetoplastLeishmaniaMorphotypeNonhumanNyssomyiaNyssomyia AntunesiPolymerase Chain ReactionPsychodidaePsychodopygusPsychodopygus DavisiPolymorphism, Restriction Fragment LengthSkin LeishmaniasisSpecies RichnessTracheitisTrichophoromyia UbiquitalisAnimalsDisease CarrierEcosystemGene Expression RegulationGeneticsLeishmaniaMetabolismParasitologyPhysiologyPsychodidaeSpecies DifferenceDipteraHexapodaKinetoplastidaPhlebotominaePsychodidaeTrypanosomatidaeAnimalBrasilDna, ProtozoanEcosystemFemaleGene Expression RegulationHsp72 Heat-shock ProteinsInsect VectorsLeishmaniaPolymerase Chain ReactionPolymorphism, Restriction Fragment LengthPsychodidaeSpecies SpecificityEcological aspects and molecular detection of Leishmania DNA Ross (Kinetoplastida: Trypanosomatidae) in phlebotomine sandflies (Diptera: Psychodidae) in terra firme and várzea environments in the Middle Solimões Region, Amazonas State, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleParasites and Vectorsengreponame:Repositório Institucional do INPAinstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPAORIGINALartigo-inpa.pdfartigo-inpa.pdfapplication/pdf3898513https://repositorio.inpa.gov.br/bitstream/1/15923/1/artigo-inpa.pdf0ef64e8883a4e7031214c2d81db730c0MD511/159232020-05-20 10:28:16.606oai:repositorio:1/15923Repositório de PublicaçõesPUBhttps://repositorio.inpa.gov.br/oai/requestopendoar:2020-05-20T14:28:16Repositório Institucional do INPA - Instituto Nacional de Pesquisas da Amazônia (INPA)false |
dc.title.en.fl_str_mv |
Ecological aspects and molecular detection of Leishmania DNA Ross (Kinetoplastida: Trypanosomatidae) in phlebotomine sandflies (Diptera: Psychodidae) in terra firme and várzea environments in the Middle Solimões Region, Amazonas State, Brazil |
title |
Ecological aspects and molecular detection of Leishmania DNA Ross (Kinetoplastida: Trypanosomatidae) in phlebotomine sandflies (Diptera: Psychodidae) in terra firme and várzea environments in the Middle Solimões Region, Amazonas State, Brazil |
spellingShingle |
Ecological aspects and molecular detection of Leishmania DNA Ross (Kinetoplastida: Trypanosomatidae) in phlebotomine sandflies (Diptera: Psychodidae) in terra firme and várzea environments in the Middle Solimões Region, Amazonas State, Brazil Pereira Júnior, Antonio Marques Dna Heat Shock Protein 72 Protozoal Dna Brasil Disease Transmission Dna Extraction Dna Sequence Ecological Phenomena And Functions Female Gene Sequence Human Kinetoplast Leishmania Morphotype Nonhuman Nyssomyia Nyssomyia Antunesi Polymerase Chain Reaction Psychodidae Psychodopygus Psychodopygus Davisi Polymorphism, Restriction Fragment Length Skin Leishmaniasis Species Richness Tracheitis Trichophoromyia Ubiquitalis Animals Disease Carrier Ecosystem Gene Expression Regulation Genetics Leishmania Metabolism Parasitology Physiology Psychodidae Species Difference Diptera Hexapoda Kinetoplastida Phlebotominae Psychodidae Trypanosomatidae Animal Brasil Dna, Protozoan Ecosystem Female Gene Expression Regulation Hsp72 Heat-shock Proteins Insect Vectors Leishmania Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Psychodidae Species Specificity |
title_short |
Ecological aspects and molecular detection of Leishmania DNA Ross (Kinetoplastida: Trypanosomatidae) in phlebotomine sandflies (Diptera: Psychodidae) in terra firme and várzea environments in the Middle Solimões Region, Amazonas State, Brazil |
title_full |
Ecological aspects and molecular detection of Leishmania DNA Ross (Kinetoplastida: Trypanosomatidae) in phlebotomine sandflies (Diptera: Psychodidae) in terra firme and várzea environments in the Middle Solimões Region, Amazonas State, Brazil |
title_fullStr |
Ecological aspects and molecular detection of Leishmania DNA Ross (Kinetoplastida: Trypanosomatidae) in phlebotomine sandflies (Diptera: Psychodidae) in terra firme and várzea environments in the Middle Solimões Region, Amazonas State, Brazil |
title_full_unstemmed |
Ecological aspects and molecular detection of Leishmania DNA Ross (Kinetoplastida: Trypanosomatidae) in phlebotomine sandflies (Diptera: Psychodidae) in terra firme and várzea environments in the Middle Solimões Region, Amazonas State, Brazil |
title_sort |
Ecological aspects and molecular detection of Leishmania DNA Ross (Kinetoplastida: Trypanosomatidae) in phlebotomine sandflies (Diptera: Psychodidae) in terra firme and várzea environments in the Middle Solimões Region, Amazonas State, Brazil |
author |
Pereira Júnior, Antonio Marques |
author_facet |
Pereira Júnior, Antonio Marques Teles, Carolina Bioni Garcia Azevedo dos Santos, Ana Paula Silva de Rodrigues, Moreno S. Marialva, Eric Fabrício Pessoa, Felipe Arley Costa Medeiros, Jansen Fernandes de |
author_role |
author |
author2 |
Teles, Carolina Bioni Garcia Azevedo dos Santos, Ana Paula Silva de Rodrigues, Moreno S. Marialva, Eric Fabrício Pessoa, Felipe Arley Costa Medeiros, Jansen Fernandes de |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Pereira Júnior, Antonio Marques Teles, Carolina Bioni Garcia Azevedo dos Santos, Ana Paula Silva de Rodrigues, Moreno S. Marialva, Eric Fabrício Pessoa, Felipe Arley Costa Medeiros, Jansen Fernandes de |
dc.subject.eng.fl_str_mv |
Dna Heat Shock Protein 72 Protozoal Dna Brasil Disease Transmission Dna Extraction Dna Sequence Ecological Phenomena And Functions Female Gene Sequence Human Kinetoplast Leishmania Morphotype Nonhuman Nyssomyia Nyssomyia Antunesi Polymerase Chain Reaction Psychodidae Psychodopygus Psychodopygus Davisi Polymorphism, Restriction Fragment Length Skin Leishmaniasis Species Richness Tracheitis Trichophoromyia Ubiquitalis Animals Disease Carrier Ecosystem Gene Expression Regulation Genetics Leishmania Metabolism Parasitology Physiology Psychodidae Species Difference Diptera Hexapoda Kinetoplastida Phlebotominae Psychodidae Trypanosomatidae Animal Brasil Dna, Protozoan Ecosystem Female Gene Expression Regulation Hsp72 Heat-shock Proteins Insect Vectors Leishmania Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Psychodidae Species Specificity |
topic |
Dna Heat Shock Protein 72 Protozoal Dna Brasil Disease Transmission Dna Extraction Dna Sequence Ecological Phenomena And Functions Female Gene Sequence Human Kinetoplast Leishmania Morphotype Nonhuman Nyssomyia Nyssomyia Antunesi Polymerase Chain Reaction Psychodidae Psychodopygus Psychodopygus Davisi Polymorphism, Restriction Fragment Length Skin Leishmaniasis Species Richness Tracheitis Trichophoromyia Ubiquitalis Animals Disease Carrier Ecosystem Gene Expression Regulation Genetics Leishmania Metabolism Parasitology Physiology Psychodidae Species Difference Diptera Hexapoda Kinetoplastida Phlebotominae Psychodidae Trypanosomatidae Animal Brasil Dna, Protozoan Ecosystem Female Gene Expression Regulation Hsp72 Heat-shock Proteins Insect Vectors Leishmania Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Psychodidae Species Specificity |
description |
Background: Phlebotomine sand flies (Diptera: Psychodidae) are insects of medical importance due to the role that some species play in the transmission of leishmaniasis. This work aimed to study some ecological aspects among sand flies fauna inhabiting two different environments: the várzea (lowland Amazonian forest) and terra firme (upland Amazonian forest), both located in Tefé Municipality, Amazonas State, Braziland to detect Leishmania infection in those phlebotomine populations. Methods: Sand flies were collected using HP light traps. Collection took place over the course of six months: January, February, April, August, September, and October of 2013. To detect natural infection by Leishmania, DNA samples were extracted from female sand flies and submitted to Polymerase Chain Reaction (PCR) targeting the kDNA gene; Leishmania species were identified by PCR-RFLP targeting the hsp70 gene and genetic sequencing. Results: In all, 5,716 individuals were collected, and 46 species were identified. Trichophoromyia ubiquitalis (3,330 - 58.26%) and Nyssomyia antunesi (661 - 11.26%) were the most abundant species. Species richness was greater in terra firme environments (42 species) than in the várzea environments (22 species), and forests ecotopes (43 species) were richer than peridomiciles (28 species). DNA of Leishmania was found in Th. ubiquitalis and Psychodopygus davisi, both of which inhabit the terra firme environment and sequencing analysis confirmed the presence of Leishmania (Viannia) lainsoni DNA in Th. ubiquitalis in Tefé Municipality. Conclusions: The high abundance of Th. ubiquitalis and Ps. davisi and detection of DNA of Leishmania sp. may indicate that both species could be putative vectors for American Cutaneous Leishmaniasis (ACL) in the terra firme environment of Tefé. The sand fly fauna found in várzea is rich and diverse, exhibiting several species, nevertheless the seasonal hydric stress during part of the year that could influence the local diversity, if compared with other studies. This is the first report in Amazonas State of Th. ubiquitalis with presence of L. (V.) lainsoni DNA. © 2015 Pereira et al.; licensee BioMed Central. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015 |
dc.date.accessioned.fl_str_mv |
2020-05-20T14:15:56Z |
dc.date.available.fl_str_mv |
2020-05-20T14:15:56Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.inpa.gov.br/handle/1/15923 |
dc.identifier.doi.none.fl_str_mv |
10.1186/s13071-015-0789-2 |
url |
https://repositorio.inpa.gov.br/handle/1/15923 |
identifier_str_mv |
10.1186/s13071-015-0789-2 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Volume 8, Número 1 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Parasites and Vectors |
publisher.none.fl_str_mv |
Parasites and Vectors |
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reponame:Repositório Institucional do INPA instname:Instituto Nacional de Pesquisas da Amazônia (INPA) instacron:INPA |
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INPA |
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INPA |
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Repositório Institucional do INPA |
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Repositório Institucional do INPA |
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https://repositorio.inpa.gov.br/bitstream/1/15923/1/artigo-inpa.pdf |
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