Transferability of heterologous primers in Brycon falcatus
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Amazonica |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672020000300232 |
Resumo: | ABSTRACT The genusBryconcomprises fish species of significant socioeconomic and biological importance in Brazil. Despite that, the genetic knowledge about these species is scarce, especially regardingBrycon falcatus. Thus, the objective of this study was to evaluate the transferability of heterologous microsatellite primers inB. falcatus for the first time. Heterologous primers obtained from B. opalinus, B. hilarii, B. insignis, B. orbignyanus, B. amazonicus, Prochilodus argenteus, Prochilodus lineatus, Piaractus mesopotamicus, and Colossoma macropomum were evaluated. The primers that showed the best amplification patterns were applied to a sample of 22 individuals and the genetic parameters were calculated. Nine primers displayed satisfactory cross-amplification withB. falcatus: BoM5 (Brycon opalinus); Bh8, Bh13 and Bh16 (B. hilarii); Borg59 (B. orbignyanus); Bag22 (B. amazonicus); Par12 and Par80 (P. argenteus), and Cm1A8 (C. macropomum). The genetic parameters (number of alleles, effective alleles, allele richness, and expected and observed heterozygosity) and the polymorphic information content (PIC) confirmed the viability of these primers for population genetics analyses. Our study demonstrates the potential of transferability of microsatellite markers from related species and even different genera to B. falcatus, providing usefull tools for future population genetic studies in this species. |
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Transferability of heterologous primers in Brycon falcatusBryconidaecross amplificationgenetic conservationgenetic variabilitySSRABSTRACT The genusBryconcomprises fish species of significant socioeconomic and biological importance in Brazil. Despite that, the genetic knowledge about these species is scarce, especially regardingBrycon falcatus. Thus, the objective of this study was to evaluate the transferability of heterologous microsatellite primers inB. falcatus for the first time. Heterologous primers obtained from B. opalinus, B. hilarii, B. insignis, B. orbignyanus, B. amazonicus, Prochilodus argenteus, Prochilodus lineatus, Piaractus mesopotamicus, and Colossoma macropomum were evaluated. The primers that showed the best amplification patterns were applied to a sample of 22 individuals and the genetic parameters were calculated. Nine primers displayed satisfactory cross-amplification withB. falcatus: BoM5 (Brycon opalinus); Bh8, Bh13 and Bh16 (B. hilarii); Borg59 (B. orbignyanus); Bag22 (B. amazonicus); Par12 and Par80 (P. argenteus), and Cm1A8 (C. macropomum). The genetic parameters (number of alleles, effective alleles, allele richness, and expected and observed heterozygosity) and the polymorphic information content (PIC) confirmed the viability of these primers for population genetics analyses. Our study demonstrates the potential of transferability of microsatellite markers from related species and even different genera to B. falcatus, providing usefull tools for future population genetic studies in this species.Instituto Nacional de Pesquisas da Amazônia2020-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672020000300232Acta Amazonica v.50 n.3 2020reponame:Acta Amazonicainstname:Instituto Nacional de Pesquisas da Amazônia (INPA)instacron:INPA10.1590/1809-4392201904191info:eu-repo/semantics/openAccessPENHA,Diego dos SantosSOUZA,Felipe Pinheiro deLIMA,Ed Christian Suzuki deURREA-ROJAS,Angela MariaPANDOLFI,Victor César FreitasYAMACHITA,Andrei LincolnPOVH,Jayme AparecidoLEITE,Natalia GonçalvesPEREIRA,Ulisses de PáduaLOPERA-BARRERO,Nelson Mauricioeng2020-11-30T00:00:00Zoai:scielo:S0044-59672020000300232Revistahttps://acta.inpa.gov.br/PUBhttps://old.scielo.br/oai/scielo-oai.phpacta@inpa.gov.br||acta@inpa.gov.br1809-43920044-5967opendoar:2020-11-30T00:00Acta Amazonica - Instituto Nacional de Pesquisas da Amazônia (INPA)false |
dc.title.none.fl_str_mv |
Transferability of heterologous primers in Brycon falcatus |
title |
Transferability of heterologous primers in Brycon falcatus |
spellingShingle |
Transferability of heterologous primers in Brycon falcatus PENHA,Diego dos Santos Bryconidae cross amplification genetic conservation genetic variability SSR |
title_short |
Transferability of heterologous primers in Brycon falcatus |
title_full |
Transferability of heterologous primers in Brycon falcatus |
title_fullStr |
Transferability of heterologous primers in Brycon falcatus |
title_full_unstemmed |
Transferability of heterologous primers in Brycon falcatus |
title_sort |
Transferability of heterologous primers in Brycon falcatus |
author |
PENHA,Diego dos Santos |
author_facet |
PENHA,Diego dos Santos SOUZA,Felipe Pinheiro de LIMA,Ed Christian Suzuki de URREA-ROJAS,Angela Maria PANDOLFI,Victor César Freitas YAMACHITA,Andrei Lincoln POVH,Jayme Aparecido LEITE,Natalia Gonçalves PEREIRA,Ulisses de Pádua LOPERA-BARRERO,Nelson Mauricio |
author_role |
author |
author2 |
SOUZA,Felipe Pinheiro de LIMA,Ed Christian Suzuki de URREA-ROJAS,Angela Maria PANDOLFI,Victor César Freitas YAMACHITA,Andrei Lincoln POVH,Jayme Aparecido LEITE,Natalia Gonçalves PEREIRA,Ulisses de Pádua LOPERA-BARRERO,Nelson Mauricio |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
PENHA,Diego dos Santos SOUZA,Felipe Pinheiro de LIMA,Ed Christian Suzuki de URREA-ROJAS,Angela Maria PANDOLFI,Victor César Freitas YAMACHITA,Andrei Lincoln POVH,Jayme Aparecido LEITE,Natalia Gonçalves PEREIRA,Ulisses de Pádua LOPERA-BARRERO,Nelson Mauricio |
dc.subject.por.fl_str_mv |
Bryconidae cross amplification genetic conservation genetic variability SSR |
topic |
Bryconidae cross amplification genetic conservation genetic variability SSR |
description |
ABSTRACT The genusBryconcomprises fish species of significant socioeconomic and biological importance in Brazil. Despite that, the genetic knowledge about these species is scarce, especially regardingBrycon falcatus. Thus, the objective of this study was to evaluate the transferability of heterologous microsatellite primers inB. falcatus for the first time. Heterologous primers obtained from B. opalinus, B. hilarii, B. insignis, B. orbignyanus, B. amazonicus, Prochilodus argenteus, Prochilodus lineatus, Piaractus mesopotamicus, and Colossoma macropomum were evaluated. The primers that showed the best amplification patterns were applied to a sample of 22 individuals and the genetic parameters were calculated. Nine primers displayed satisfactory cross-amplification withB. falcatus: BoM5 (Brycon opalinus); Bh8, Bh13 and Bh16 (B. hilarii); Borg59 (B. orbignyanus); Bag22 (B. amazonicus); Par12 and Par80 (P. argenteus), and Cm1A8 (C. macropomum). The genetic parameters (number of alleles, effective alleles, allele richness, and expected and observed heterozygosity) and the polymorphic information content (PIC) confirmed the viability of these primers for population genetics analyses. Our study demonstrates the potential of transferability of microsatellite markers from related species and even different genera to B. falcatus, providing usefull tools for future population genetic studies in this species. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672020000300232 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672020000300232 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1809-4392201904191 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Nacional de Pesquisas da Amazônia |
publisher.none.fl_str_mv |
Instituto Nacional de Pesquisas da Amazônia |
dc.source.none.fl_str_mv |
Acta Amazonica v.50 n.3 2020 reponame:Acta Amazonica instname:Instituto Nacional de Pesquisas da Amazônia (INPA) instacron:INPA |
instname_str |
Instituto Nacional de Pesquisas da Amazônia (INPA) |
instacron_str |
INPA |
institution |
INPA |
reponame_str |
Acta Amazonica |
collection |
Acta Amazonica |
repository.name.fl_str_mv |
Acta Amazonica - Instituto Nacional de Pesquisas da Amazônia (INPA) |
repository.mail.fl_str_mv |
acta@inpa.gov.br||acta@inpa.gov.br |
_version_ |
1752129841237327872 |