Evaluation of endoglucanase and β-glucosidase production by bacteria and yeasts isolated from a eucalyptus plantation in the cerrado of Minas Gerais
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Ambiente & Água |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1980-993X2019000400300 |
Resumo: | Abstract The current global environmental and economic scenario is intrinsically related to the increase in fossil fuel consumption caused by technological development and world population growth. Thus, it is necessary to search for renewable sources of biofuel in an attempt to mitigate the effects of fossil fuels on the environment and the lack of these non-renewable fuels. The use of lignocellulosic biomass, an abundant and renewable resource in Brazilian regions, has contributed successfully to new research and technologies for second-generation ethanol production. The conversion of lignocellulosic biomass into fermentable sugars requires the use of cellulolytic enzymes produced by microorganisms found in the microbiota. This work evaluated the production of endoglucanase and β-glucosidase in a liquid medium containing carboxymethylcellulose by five microorganisms (bacteria and yeasts) from the bank of 348 isolates from eucalyptus soils in the Cerrado Mineiro. Microorganisms with the following enzymatic activity indexes were selected: IM1-74 (22), IM25-9 (5.33), IM32-90 (7.33), IM1-5 (10.33) and IM32-91 (5.44). The microorganisms with the highest enzymatic activity in the liquid medium were IM32-90 (endoglucanase = 0.214 U mL-1) and IM32-91 (β-glucosidase = 0.067 U mL-1). |
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Evaluation of endoglucanase and β-glucosidase production by bacteria and yeasts isolated from a eucalyptus plantation in the cerrado of Minas Geraiscellulaseenzymesrenewable sourcesAbstract The current global environmental and economic scenario is intrinsically related to the increase in fossil fuel consumption caused by technological development and world population growth. Thus, it is necessary to search for renewable sources of biofuel in an attempt to mitigate the effects of fossil fuels on the environment and the lack of these non-renewable fuels. The use of lignocellulosic biomass, an abundant and renewable resource in Brazilian regions, has contributed successfully to new research and technologies for second-generation ethanol production. The conversion of lignocellulosic biomass into fermentable sugars requires the use of cellulolytic enzymes produced by microorganisms found in the microbiota. This work evaluated the production of endoglucanase and β-glucosidase in a liquid medium containing carboxymethylcellulose by five microorganisms (bacteria and yeasts) from the bank of 348 isolates from eucalyptus soils in the Cerrado Mineiro. Microorganisms with the following enzymatic activity indexes were selected: IM1-74 (22), IM25-9 (5.33), IM32-90 (7.33), IM1-5 (10.33) and IM32-91 (5.44). The microorganisms with the highest enzymatic activity in the liquid medium were IM32-90 (endoglucanase = 0.214 U mL-1) and IM32-91 (β-glucosidase = 0.067 U mL-1).Instituto de Pesquisas Ambientais em Bacias Hidrográficas2019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1980-993X2019000400300Revista Ambiente & Água v.14 n.4 2019reponame:Revista Ambiente & Águainstname:Instituto de Pesquisas Ambientais em Bacias Hidrográficas (IPABHI)instacron:IPABHI10.4136/ambi-agua.2324info:eu-repo/semantics/openAccessMenezes,Farley Souza RibeiroCruz,Gizelly Gomes daLopes,Matheus de OliveiraNelson,David LeeMartins,Tamira Quintiliana GomesLaia,Marcelo Luiz deeng2019-07-10T00:00:00Zoai:scielo:S1980-993X2019000400300Revistahttp://www.ambi-agua.net/PUBhttps://old.scielo.br/oai/scielo-oai.php||ambi.agua@gmail.com1980-993X1980-993Xopendoar:2019-07-10T00:00Revista Ambiente & Água - Instituto de Pesquisas Ambientais em Bacias Hidrográficas (IPABHI)false |
dc.title.none.fl_str_mv |
Evaluation of endoglucanase and β-glucosidase production by bacteria and yeasts isolated from a eucalyptus plantation in the cerrado of Minas Gerais |
title |
Evaluation of endoglucanase and β-glucosidase production by bacteria and yeasts isolated from a eucalyptus plantation in the cerrado of Minas Gerais |
spellingShingle |
Evaluation of endoglucanase and β-glucosidase production by bacteria and yeasts isolated from a eucalyptus plantation in the cerrado of Minas Gerais Menezes,Farley Souza Ribeiro cellulase enzymes renewable sources |
title_short |
Evaluation of endoglucanase and β-glucosidase production by bacteria and yeasts isolated from a eucalyptus plantation in the cerrado of Minas Gerais |
title_full |
Evaluation of endoglucanase and β-glucosidase production by bacteria and yeasts isolated from a eucalyptus plantation in the cerrado of Minas Gerais |
title_fullStr |
Evaluation of endoglucanase and β-glucosidase production by bacteria and yeasts isolated from a eucalyptus plantation in the cerrado of Minas Gerais |
title_full_unstemmed |
Evaluation of endoglucanase and β-glucosidase production by bacteria and yeasts isolated from a eucalyptus plantation in the cerrado of Minas Gerais |
title_sort |
Evaluation of endoglucanase and β-glucosidase production by bacteria and yeasts isolated from a eucalyptus plantation in the cerrado of Minas Gerais |
author |
Menezes,Farley Souza Ribeiro |
author_facet |
Menezes,Farley Souza Ribeiro Cruz,Gizelly Gomes da Lopes,Matheus de Oliveira Nelson,David Lee Martins,Tamira Quintiliana Gomes Laia,Marcelo Luiz de |
author_role |
author |
author2 |
Cruz,Gizelly Gomes da Lopes,Matheus de Oliveira Nelson,David Lee Martins,Tamira Quintiliana Gomes Laia,Marcelo Luiz de |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Menezes,Farley Souza Ribeiro Cruz,Gizelly Gomes da Lopes,Matheus de Oliveira Nelson,David Lee Martins,Tamira Quintiliana Gomes Laia,Marcelo Luiz de |
dc.subject.por.fl_str_mv |
cellulase enzymes renewable sources |
topic |
cellulase enzymes renewable sources |
description |
Abstract The current global environmental and economic scenario is intrinsically related to the increase in fossil fuel consumption caused by technological development and world population growth. Thus, it is necessary to search for renewable sources of biofuel in an attempt to mitigate the effects of fossil fuels on the environment and the lack of these non-renewable fuels. The use of lignocellulosic biomass, an abundant and renewable resource in Brazilian regions, has contributed successfully to new research and technologies for second-generation ethanol production. The conversion of lignocellulosic biomass into fermentable sugars requires the use of cellulolytic enzymes produced by microorganisms found in the microbiota. This work evaluated the production of endoglucanase and β-glucosidase in a liquid medium containing carboxymethylcellulose by five microorganisms (bacteria and yeasts) from the bank of 348 isolates from eucalyptus soils in the Cerrado Mineiro. Microorganisms with the following enzymatic activity indexes were selected: IM1-74 (22), IM25-9 (5.33), IM32-90 (7.33), IM1-5 (10.33) and IM32-91 (5.44). The microorganisms with the highest enzymatic activity in the liquid medium were IM32-90 (endoglucanase = 0.214 U mL-1) and IM32-91 (β-glucosidase = 0.067 U mL-1). |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1980-993X2019000400300 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1980-993X2019000400300 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.4136/ambi-agua.2324 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Pesquisas Ambientais em Bacias Hidrográficas |
publisher.none.fl_str_mv |
Instituto de Pesquisas Ambientais em Bacias Hidrográficas |
dc.source.none.fl_str_mv |
Revista Ambiente & Água v.14 n.4 2019 reponame:Revista Ambiente & Água instname:Instituto de Pesquisas Ambientais em Bacias Hidrográficas (IPABHI) instacron:IPABHI |
instname_str |
Instituto de Pesquisas Ambientais em Bacias Hidrográficas (IPABHI) |
instacron_str |
IPABHI |
institution |
IPABHI |
reponame_str |
Revista Ambiente & Água |
collection |
Revista Ambiente & Água |
repository.name.fl_str_mv |
Revista Ambiente & Água - Instituto de Pesquisas Ambientais em Bacias Hidrográficas (IPABHI) |
repository.mail.fl_str_mv |
||ambi.agua@gmail.com |
_version_ |
1752129750822813696 |