Application of a voltammetric enzymatic biosensor based on crude extract of Marasmiellus colocasiae for the detection of phenolic compounds in drinking water
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Ambiente & Água |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1980-993X2020000600311 |
Resumo: | Abstract The chemical and pharmaceutical industries are the main generators of residues, such as phenolic microcontaminants, including catechol, resorcinol, p-nitrophenol and 4-chlorophenol. Therefore, this work aims to identify these microcontaminants through an emerging contaminant biosensor by means of an enzymatic biosensor constructed with an enzymatic extract from the fungus Marasmiellus colocasiae. Based on the differential pulse voltammetry electrochemical technique, the biosensor was used to analyze the patterns of catechol, resorcinol, p-nitrophenol and 4-chlorophenol. The analysis of a sample prepared with these standards in water from the public supply network was also carried out. As a result, it was possible to verify that the biosensor developed in this study is more sensitive than conventional methods and has a greater affinity for catechol. In the sample prepared with the standards, it was possible to qualitatively identify the presence of 4-chlorophenol, resorcinol and catechol. The proposed biosensor was sensitive and has potential for application in the analysis of microcontaminants in the environment with the detection limit = 0.17 µmol L-1, and the quantification limit = 0.52 µmol L-1. |
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Application of a voltammetric enzymatic biosensor based on crude extract of Marasmiellus colocasiae for the detection of phenolic compounds in drinking waterelectrochemical biosensormicropollutantspolyphenol oxidasespotable waterAbstract The chemical and pharmaceutical industries are the main generators of residues, such as phenolic microcontaminants, including catechol, resorcinol, p-nitrophenol and 4-chlorophenol. Therefore, this work aims to identify these microcontaminants through an emerging contaminant biosensor by means of an enzymatic biosensor constructed with an enzymatic extract from the fungus Marasmiellus colocasiae. Based on the differential pulse voltammetry electrochemical technique, the biosensor was used to analyze the patterns of catechol, resorcinol, p-nitrophenol and 4-chlorophenol. The analysis of a sample prepared with these standards in water from the public supply network was also carried out. As a result, it was possible to verify that the biosensor developed in this study is more sensitive than conventional methods and has a greater affinity for catechol. In the sample prepared with the standards, it was possible to qualitatively identify the presence of 4-chlorophenol, resorcinol and catechol. The proposed biosensor was sensitive and has potential for application in the analysis of microcontaminants in the environment with the detection limit = 0.17 µmol L-1, and the quantification limit = 0.52 µmol L-1.Instituto de Pesquisas Ambientais em Bacias Hidrográficas2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1980-993X2020000600311Revista Ambiente & Água v.15 n.6 2020reponame:Revista Ambiente & Águainstname:Instituto de Pesquisas Ambientais em Bacias Hidrográficas (IPABHI)instacron:IPABHI10.4136/ambi-agua.2610info:eu-repo/semantics/openAccessBatista,Érica AparecidaGarcia,Luane FerreiraAlbuquerque,Antonio João Carvalho deBallaminut,NaraScalize,Paulo SérgioGil,Eric Souzaeng2020-11-24T00:00:00Zoai:scielo:S1980-993X2020000600311Revistahttp://www.ambi-agua.net/PUBhttps://old.scielo.br/oai/scielo-oai.php||ambi.agua@gmail.com1980-993X1980-993Xopendoar:2020-11-24T00:00Revista Ambiente & Água - Instituto de Pesquisas Ambientais em Bacias Hidrográficas (IPABHI)false |
dc.title.none.fl_str_mv |
Application of a voltammetric enzymatic biosensor based on crude extract of Marasmiellus colocasiae for the detection of phenolic compounds in drinking water |
title |
Application of a voltammetric enzymatic biosensor based on crude extract of Marasmiellus colocasiae for the detection of phenolic compounds in drinking water |
spellingShingle |
Application of a voltammetric enzymatic biosensor based on crude extract of Marasmiellus colocasiae for the detection of phenolic compounds in drinking water Batista,Érica Aparecida electrochemical biosensor micropollutants polyphenol oxidases potable water |
title_short |
Application of a voltammetric enzymatic biosensor based on crude extract of Marasmiellus colocasiae for the detection of phenolic compounds in drinking water |
title_full |
Application of a voltammetric enzymatic biosensor based on crude extract of Marasmiellus colocasiae for the detection of phenolic compounds in drinking water |
title_fullStr |
Application of a voltammetric enzymatic biosensor based on crude extract of Marasmiellus colocasiae for the detection of phenolic compounds in drinking water |
title_full_unstemmed |
Application of a voltammetric enzymatic biosensor based on crude extract of Marasmiellus colocasiae for the detection of phenolic compounds in drinking water |
title_sort |
Application of a voltammetric enzymatic biosensor based on crude extract of Marasmiellus colocasiae for the detection of phenolic compounds in drinking water |
author |
Batista,Érica Aparecida |
author_facet |
Batista,Érica Aparecida Garcia,Luane Ferreira Albuquerque,Antonio João Carvalho de Ballaminut,Nara Scalize,Paulo Sérgio Gil,Eric Souza |
author_role |
author |
author2 |
Garcia,Luane Ferreira Albuquerque,Antonio João Carvalho de Ballaminut,Nara Scalize,Paulo Sérgio Gil,Eric Souza |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Batista,Érica Aparecida Garcia,Luane Ferreira Albuquerque,Antonio João Carvalho de Ballaminut,Nara Scalize,Paulo Sérgio Gil,Eric Souza |
dc.subject.por.fl_str_mv |
electrochemical biosensor micropollutants polyphenol oxidases potable water |
topic |
electrochemical biosensor micropollutants polyphenol oxidases potable water |
description |
Abstract The chemical and pharmaceutical industries are the main generators of residues, such as phenolic microcontaminants, including catechol, resorcinol, p-nitrophenol and 4-chlorophenol. Therefore, this work aims to identify these microcontaminants through an emerging contaminant biosensor by means of an enzymatic biosensor constructed with an enzymatic extract from the fungus Marasmiellus colocasiae. Based on the differential pulse voltammetry electrochemical technique, the biosensor was used to analyze the patterns of catechol, resorcinol, p-nitrophenol and 4-chlorophenol. The analysis of a sample prepared with these standards in water from the public supply network was also carried out. As a result, it was possible to verify that the biosensor developed in this study is more sensitive than conventional methods and has a greater affinity for catechol. In the sample prepared with the standards, it was possible to qualitatively identify the presence of 4-chlorophenol, resorcinol and catechol. The proposed biosensor was sensitive and has potential for application in the analysis of microcontaminants in the environment with the detection limit = 0.17 µmol L-1, and the quantification limit = 0.52 µmol L-1. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1980-993X2020000600311 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1980-993X2020000600311 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.4136/ambi-agua.2610 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Pesquisas Ambientais em Bacias Hidrográficas |
publisher.none.fl_str_mv |
Instituto de Pesquisas Ambientais em Bacias Hidrográficas |
dc.source.none.fl_str_mv |
Revista Ambiente & Água v.15 n.6 2020 reponame:Revista Ambiente & Água instname:Instituto de Pesquisas Ambientais em Bacias Hidrográficas (IPABHI) instacron:IPABHI |
instname_str |
Instituto de Pesquisas Ambientais em Bacias Hidrográficas (IPABHI) |
instacron_str |
IPABHI |
institution |
IPABHI |
reponame_str |
Revista Ambiente & Água |
collection |
Revista Ambiente & Água |
repository.name.fl_str_mv |
Revista Ambiente & Água - Instituto de Pesquisas Ambientais em Bacias Hidrográficas (IPABHI) |
repository.mail.fl_str_mv |
||ambi.agua@gmail.com |
_version_ |
1752129751332421632 |