Effects of an inhibitor of nitric oxide production on cell cycle and micronucleus frequency in irradiated human breast adenocarcinoma cells
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo de conferência |
Título da fonte: | Repositório Institucional do IPEN |
Texto Completo: | http://repositorio.ipen.br/handle/123456789/30576 |
Resumo: | Breast adenocarcinomas ar e the most frequent malignant tumor (about 25% of cases), and its malignant outcome causes about 15% of all deaths of women with cancer. The production of nitric oxide (NO) by isoforms of nitric ox ide synthases (NOS???s) are related to increased malignancy a nd stimuli to metastatic progression of breast adenocarcinomas, but its presence in irradiated cells can lead to higher frequencies of DNA damage. The work used aminoguanidine, an inhibitor of isof orm 2 of NOS (NOS 2) to treat human breast cancer cells (MCF 7) in non toxic concentrations (1 and 2mM) before exposure to gamma irradiation 60 Co) to assess if reduction of intracellular NO can protect from, or induce radio induced cell damage, cell cycle disruption or death. Cells were treated and irradiated at 0, 0.5, 1, 2, 4 or 8Gy . A dministration of aminoguanidine arrest ed the cell cycle in the synthesis (S) phase, altering the DNA repair capacity of cells. T he higher concentration (2mM) led to less genotoxic damage (about 50%) in cells irradiated at 8Gy as obse rved using micronucleus scoring by flow cytometry. Alternatively, 1mM of aminoguanidin e increased genotoxic damage and induced a less significant increase of S phase cells. Despite the findings, no significant alterations in cell proliferation rates were o bserved. Finding s showed that aminogua ni dine can modulate radio induced ef fects on cells. |
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2020-01-06T14:43:57Z2020-01-06T14:43:57ZOctober 21-25, 2019http://repositorio.ipen.br/handle/123456789/305760000-0002-0007-534XBreast adenocarcinomas ar e the most frequent malignant tumor (about 25% of cases), and its malignant outcome causes about 15% of all deaths of women with cancer. The production of nitric oxide (NO) by isoforms of nitric ox ide synthases (NOS???s) are related to increased malignancy a nd stimuli to metastatic progression of breast adenocarcinomas, but its presence in irradiated cells can lead to higher frequencies of DNA damage. The work used aminoguanidine, an inhibitor of isof orm 2 of NOS (NOS 2) to treat human breast cancer cells (MCF 7) in non toxic concentrations (1 and 2mM) before exposure to gamma irradiation 60 Co) to assess if reduction of intracellular NO can protect from, or induce radio induced cell damage, cell cycle disruption or death. Cells were treated and irradiated at 0, 0.5, 1, 2, 4 or 8Gy . A dministration of aminoguanidine arrest ed the cell cycle in the synthesis (S) phase, altering the DNA repair capacity of cells. T he higher concentration (2mM) led to less genotoxic damage (about 50%) in cells irradiated at 8Gy as obse rved using micronucleus scoring by flow cytometry. Alternatively, 1mM of aminoguanidin e increased genotoxic damage and induced a less significant increase of S phase cells. Despite the findings, no significant alterations in cell proliferation rates were o bserved. Finding s showed that aminogua ni dine can modulate radio induced ef fects on cells.Submitted by Celia Satomi Uehara (celia.u-topservice@ipen.br) on 2020-01-06T14:43:57Z No. of bitstreams: 1 26226.pdf: 570450 bytes, checksum: f4ac5cdc7cc23e97e1cbcb026473cfdc (MD5)Made available in DSpace on 2020-01-06T14:43:57Z (GMT). No. of bitstreams: 1 26226.pdf: 570450 bytes, checksum: f4ac5cdc7cc23e97e1cbcb026473cfdc (MD5)1649-1658Associa????o Brasileira de Energia Nuclearcomputerized simulationdynamical systemsinteractionsinvestmentmarketpersonnelproductionradiopharmaceuticalssupply and demandv codesbrazilian cnenEffects of an inhibitor of nitric oxide production on cell cycle and micronucleus frequency in irradiated human breast adenocarcinoma cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectINACIRio de JaneiroSantos, SP141861213314753141853158600600600600600AMARAL, KLEICY C.CARVALHO, LUMA R. deALBIERO, ANA LIGIALAUBE, RAQUELNASCIMENTO, ANA C.G.BONFIM, LETICIAVIEIRA, DANIEL P.INTERNATIONAL NUCLEAR ATLANTIC CONFERENCEinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional do IPENinstname:Instituto de Pesquisas Energéticas e Nucleares (IPEN)instacron:IPEN262262019VIEIRA, DANIEL P.BONFIM, LETICIANASCIMENTO, ANA C.G.CARVALHO, LUMA R. deAMARAL, KLEICY C.ALBIERO, ANA LIGIALAUBE, RAQUEL20-01Proceedings3158141851475312133141861505815115VIEIRA, DANIEL P.:3158:810:NBONFIM, LETICIA:14185:810:NNASCIMENTO, ANA C.G.:14753:810:NCARVALHO, LUMA R. de:12133:810:NAMARAL, KLEICY C.:14186:810:SALBIERO, ANA LIGIA:15058:-1:NLAUBE, RAQUEL:15115:-1:NORIGINAL26226.pdf26226.pdfapplication/pdf570450http://repositorio.ipen.br/bitstream/123456789/30576/1/26226.pdff4ac5cdc7cc23e97e1cbcb026473cfdcMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://repositorio.ipen.br/bitstream/123456789/30576/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52123456789/305762022-06-01 19:15:49.315oai:repositorio.ipen.br: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Repositório InstitucionalPUBhttp://repositorio.ipen.br/oai/requestbibl@ipen.bropendoar:45102022-06-01T19:15:49Repositório Institucional do IPEN - Instituto de Pesquisas Energéticas e Nucleares (IPEN)false |
dc.title.pt_BR.fl_str_mv |
Effects of an inhibitor of nitric oxide production on cell cycle and micronucleus frequency in irradiated human breast adenocarcinoma cells |
title |
Effects of an inhibitor of nitric oxide production on cell cycle and micronucleus frequency in irradiated human breast adenocarcinoma cells |
spellingShingle |
Effects of an inhibitor of nitric oxide production on cell cycle and micronucleus frequency in irradiated human breast adenocarcinoma cells AMARAL, KLEICY C. computerized simulation dynamical systems interactions investment market personnel production radiopharmaceuticals supply and demand v codes brazilian cnen |
title_short |
Effects of an inhibitor of nitric oxide production on cell cycle and micronucleus frequency in irradiated human breast adenocarcinoma cells |
title_full |
Effects of an inhibitor of nitric oxide production on cell cycle and micronucleus frequency in irradiated human breast adenocarcinoma cells |
title_fullStr |
Effects of an inhibitor of nitric oxide production on cell cycle and micronucleus frequency in irradiated human breast adenocarcinoma cells |
title_full_unstemmed |
Effects of an inhibitor of nitric oxide production on cell cycle and micronucleus frequency in irradiated human breast adenocarcinoma cells |
title_sort |
Effects of an inhibitor of nitric oxide production on cell cycle and micronucleus frequency in irradiated human breast adenocarcinoma cells |
author |
AMARAL, KLEICY C. |
author_facet |
AMARAL, KLEICY C. CARVALHO, LUMA R. de ALBIERO, ANA LIGIA LAUBE, RAQUEL NASCIMENTO, ANA C.G. BONFIM, LETICIA VIEIRA, DANIEL P. INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE |
author_role |
author |
author2 |
CARVALHO, LUMA R. de ALBIERO, ANA LIGIA LAUBE, RAQUEL NASCIMENTO, ANA C.G. BONFIM, LETICIA VIEIRA, DANIEL P. INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
AMARAL, KLEICY C. CARVALHO, LUMA R. de ALBIERO, ANA LIGIA LAUBE, RAQUEL NASCIMENTO, ANA C.G. BONFIM, LETICIA VIEIRA, DANIEL P. INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE |
dc.subject.por.fl_str_mv |
computerized simulation dynamical systems interactions investment market personnel production radiopharmaceuticals supply and demand v codes brazilian cnen |
topic |
computerized simulation dynamical systems interactions investment market personnel production radiopharmaceuticals supply and demand v codes brazilian cnen |
description |
Breast adenocarcinomas ar e the most frequent malignant tumor (about 25% of cases), and its malignant outcome causes about 15% of all deaths of women with cancer. The production of nitric oxide (NO) by isoforms of nitric ox ide synthases (NOS???s) are related to increased malignancy a nd stimuli to metastatic progression of breast adenocarcinomas, but its presence in irradiated cells can lead to higher frequencies of DNA damage. The work used aminoguanidine, an inhibitor of isof orm 2 of NOS (NOS 2) to treat human breast cancer cells (MCF 7) in non toxic concentrations (1 and 2mM) before exposure to gamma irradiation 60 Co) to assess if reduction of intracellular NO can protect from, or induce radio induced cell damage, cell cycle disruption or death. Cells were treated and irradiated at 0, 0.5, 1, 2, 4 or 8Gy . A dministration of aminoguanidine arrest ed the cell cycle in the synthesis (S) phase, altering the DNA repair capacity of cells. T he higher concentration (2mM) led to less genotoxic damage (about 50%) in cells irradiated at 8Gy as obse rved using micronucleus scoring by flow cytometry. Alternatively, 1mM of aminoguanidin e increased genotoxic damage and induced a less significant increase of S phase cells. Despite the findings, no significant alterations in cell proliferation rates were o bserved. Finding s showed that aminogua ni dine can modulate radio induced ef fects on cells. |
publishDate |
2020 |
dc.date.evento.pt_BR.fl_str_mv |
October 21-25, 2019 |
dc.date.accessioned.fl_str_mv |
2020-01-06T14:43:57Z |
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2020-01-06T14:43:57Z |
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