Duroia saccifera: in vitro germination, friable calli and identification of β-sitosterol and stigmasterol from the active extract against Mycobacterium tuberculosis
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Rodriguésia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2175-78602020000100717 |
Resumo: | Abstract Duroia saccifera (Rubiaceae) occurs in the Amazon rainforest and their extracts showed antibacterial properties. To obtain greater quantities of active substances, leaf segments from in vitro D. saccifera seedlings were used as explants for calli induction; calli were multiplied via multiple subcultures, dried and extracted with hexane followed by ethyl acetate (EtOAc) and methanol (MeOH). As D. macrophylla had been reported to produce antimycobacterial substances, we assayed calli extracts against Mycobacterium tuberculosis (H37Rv strain). Calli EtOAc extract was active, with a minimal inhibitory concentration (MIC) of ≤ 25 mg mL-1, IC90of 19.5 mg mL-1 and minimal bactericidal concentration (MBC) of 200 mg mL-1. EtOAc extract was analyzed by Thin Layer Chromatography (TLC) and Nuclear Magnetic Resonance (NMR) to determine its chemical profile, and was found to be rich in terpenes. Chromatographic fractionation of the EtOAc extract yielded a mixture of two sterols, β-sitosterol and stigmasterol (in proportion of 2:1), which were identified by 1H and 13C NMR analysis. As far as we know, this is the first report of Duroia saccifera in vitro cell culture, antituberculosis activity of calli extract and β-sitosterol and stigmasterol isolation from in vitro plant cell culture. |
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Duroia saccifera: in vitro germination, friable calli and identification of β-sitosterol and stigmasterol from the active extract against Mycobacterium tuberculosisantituberculosisphytochemistryplant tissue cultureRubiaceaesterolsAbstract Duroia saccifera (Rubiaceae) occurs in the Amazon rainforest and their extracts showed antibacterial properties. To obtain greater quantities of active substances, leaf segments from in vitro D. saccifera seedlings were used as explants for calli induction; calli were multiplied via multiple subcultures, dried and extracted with hexane followed by ethyl acetate (EtOAc) and methanol (MeOH). As D. macrophylla had been reported to produce antimycobacterial substances, we assayed calli extracts against Mycobacterium tuberculosis (H37Rv strain). Calli EtOAc extract was active, with a minimal inhibitory concentration (MIC) of ≤ 25 mg mL-1, IC90of 19.5 mg mL-1 and minimal bactericidal concentration (MBC) of 200 mg mL-1. EtOAc extract was analyzed by Thin Layer Chromatography (TLC) and Nuclear Magnetic Resonance (NMR) to determine its chemical profile, and was found to be rich in terpenes. Chromatographic fractionation of the EtOAc extract yielded a mixture of two sterols, β-sitosterol and stigmasterol (in proportion of 2:1), which were identified by 1H and 13C NMR analysis. As far as we know, this is the first report of Duroia saccifera in vitro cell culture, antituberculosis activity of calli extract and β-sitosterol and stigmasterol isolation from in vitro plant cell culture.Instituto de Pesquisas Jardim Botânico do Rio de Janeiro2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2175-78602020000100717Rodriguésia v.71 2020reponame:Rodriguésia (Online)instname:Instituto de Pesquisas Jardim Botânico do Rio de Janeiro (JBRJ)instacron:JBRJ10.1590/2175-7860202071054info:eu-repo/semantics/openAccessLozano,Stefhania AlzateSousa,Aline Bastos Brilhante deSouza,Julio Cezar deSilva,David Ribeiro daSalazar,Marcos Gabriel MacielHalicki,Priscila Cristina BartolomeuRamos,Daniela FernandesSilva,Pedro Eduardo Almeida daNunez,Cecilia Veronicaeng2020-07-17T00:00:00Zoai:scielo:S2175-78602020000100717Revistahttp://rodriguesia.jbrj.gov.br/https://old.scielo.br/oai/scielo-oai.phprodriguesia@jbrj.gov.br2175-78600370-6583opendoar:2020-07-17T00:00Rodriguésia (Online) - Instituto de Pesquisas Jardim Botânico do Rio de Janeiro (JBRJ)false |
dc.title.none.fl_str_mv |
Duroia saccifera: in vitro germination, friable calli and identification of β-sitosterol and stigmasterol from the active extract against Mycobacterium tuberculosis |
title |
Duroia saccifera: in vitro germination, friable calli and identification of β-sitosterol and stigmasterol from the active extract against Mycobacterium tuberculosis |
spellingShingle |
Duroia saccifera: in vitro germination, friable calli and identification of β-sitosterol and stigmasterol from the active extract against Mycobacterium tuberculosis Lozano,Stefhania Alzate antituberculosis phytochemistry plant tissue culture Rubiaceae sterols |
title_short |
Duroia saccifera: in vitro germination, friable calli and identification of β-sitosterol and stigmasterol from the active extract against Mycobacterium tuberculosis |
title_full |
Duroia saccifera: in vitro germination, friable calli and identification of β-sitosterol and stigmasterol from the active extract against Mycobacterium tuberculosis |
title_fullStr |
Duroia saccifera: in vitro germination, friable calli and identification of β-sitosterol and stigmasterol from the active extract against Mycobacterium tuberculosis |
title_full_unstemmed |
Duroia saccifera: in vitro germination, friable calli and identification of β-sitosterol and stigmasterol from the active extract against Mycobacterium tuberculosis |
title_sort |
Duroia saccifera: in vitro germination, friable calli and identification of β-sitosterol and stigmasterol from the active extract against Mycobacterium tuberculosis |
author |
Lozano,Stefhania Alzate |
author_facet |
Lozano,Stefhania Alzate Sousa,Aline Bastos Brilhante de Souza,Julio Cezar de Silva,David Ribeiro da Salazar,Marcos Gabriel Maciel Halicki,Priscila Cristina Bartolomeu Ramos,Daniela Fernandes Silva,Pedro Eduardo Almeida da Nunez,Cecilia Veronica |
author_role |
author |
author2 |
Sousa,Aline Bastos Brilhante de Souza,Julio Cezar de Silva,David Ribeiro da Salazar,Marcos Gabriel Maciel Halicki,Priscila Cristina Bartolomeu Ramos,Daniela Fernandes Silva,Pedro Eduardo Almeida da Nunez,Cecilia Veronica |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Lozano,Stefhania Alzate Sousa,Aline Bastos Brilhante de Souza,Julio Cezar de Silva,David Ribeiro da Salazar,Marcos Gabriel Maciel Halicki,Priscila Cristina Bartolomeu Ramos,Daniela Fernandes Silva,Pedro Eduardo Almeida da Nunez,Cecilia Veronica |
dc.subject.por.fl_str_mv |
antituberculosis phytochemistry plant tissue culture Rubiaceae sterols |
topic |
antituberculosis phytochemistry plant tissue culture Rubiaceae sterols |
description |
Abstract Duroia saccifera (Rubiaceae) occurs in the Amazon rainforest and their extracts showed antibacterial properties. To obtain greater quantities of active substances, leaf segments from in vitro D. saccifera seedlings were used as explants for calli induction; calli were multiplied via multiple subcultures, dried and extracted with hexane followed by ethyl acetate (EtOAc) and methanol (MeOH). As D. macrophylla had been reported to produce antimycobacterial substances, we assayed calli extracts against Mycobacterium tuberculosis (H37Rv strain). Calli EtOAc extract was active, with a minimal inhibitory concentration (MIC) of ≤ 25 mg mL-1, IC90of 19.5 mg mL-1 and minimal bactericidal concentration (MBC) of 200 mg mL-1. EtOAc extract was analyzed by Thin Layer Chromatography (TLC) and Nuclear Magnetic Resonance (NMR) to determine its chemical profile, and was found to be rich in terpenes. Chromatographic fractionation of the EtOAc extract yielded a mixture of two sterols, β-sitosterol and stigmasterol (in proportion of 2:1), which were identified by 1H and 13C NMR analysis. As far as we know, this is the first report of Duroia saccifera in vitro cell culture, antituberculosis activity of calli extract and β-sitosterol and stigmasterol isolation from in vitro plant cell culture. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2175-78602020000100717 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2175-78602020000100717 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/2175-7860202071054 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Pesquisas Jardim Botânico do Rio de Janeiro |
publisher.none.fl_str_mv |
Instituto de Pesquisas Jardim Botânico do Rio de Janeiro |
dc.source.none.fl_str_mv |
Rodriguésia v.71 2020 reponame:Rodriguésia (Online) instname:Instituto de Pesquisas Jardim Botânico do Rio de Janeiro (JBRJ) instacron:JBRJ |
instname_str |
Instituto de Pesquisas Jardim Botânico do Rio de Janeiro (JBRJ) |
instacron_str |
JBRJ |
institution |
JBRJ |
reponame_str |
Rodriguésia (Online) |
collection |
Rodriguésia (Online) |
repository.name.fl_str_mv |
Rodriguésia (Online) - Instituto de Pesquisas Jardim Botânico do Rio de Janeiro (JBRJ) |
repository.mail.fl_str_mv |
rodriguesia@jbrj.gov.br |
_version_ |
1752128674391392256 |