Effectiveness of sperm washing by discontinuous density gradient centrifugation to remove antibodies bound to the sperm membrane
Autor(a) principal: | |
---|---|
Data de Publicação: | 2014 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | MedicalExpress (São Paulo. Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2358-04292014000300123 |
Resumo: | OBJECTIVE: We evaluated the effectiveness of sperm washing using the discontinuous two-layer density gradient centrifugation method to remove antisperm antibodies attached to the sperm surface. METHOD: We prospectively enrolled sixty-six men with unexplained infertility who were seeking evaluation. Each patient delivered one semen specimen for the study. We determined antisperm antibody levels using the direct immunobeads test. Specimens were classified into two groups according to the pre-washing levels of antibody-bound spermatozoa: group 1 (low antisperm antibodies levels, immunobeads test < 20%; n = 54), and group 2 (high antisperm antibodies levels, immunobeads test > 20%; n = 12). Sperm washing was carried out using the discontinuous two-layer colloidal density gradient centrifugation method. Pre- and post-wash levels of antisperm antibodies were compared in the groups. RESULTS: The pre- and post-wash percentage of spermatozoa with antisperm antibodies attached to their surface was 11% and 6.5% in group 1 (mean difference = 40%; p < 0.01), and 30% and 19.5% in group 2 (mean difference = 36.8%, p = 0.02), respectively. The effectiveness of density gradient centrifugation in removing antisperm antibodies was not different between the groups, but individual variations from - 52.3% to -3.9% were observed. CONCLUSIONS: Sperm washing by density gradient centrifugation is an overall effective method to remove antibodies bound to sperm membranes, regardless of the levels of antisperm antibodies in the neat semen. Due to an inter-individual variation in the effectiveness of the method, we recommend that each patient be tested before applying sperm washing by density gradient centrifugation in intrauterine insemination. |
id |
METC-1_cb2fe9d7c51cd8536b6b147dfa4d4a9d |
---|---|
oai_identifier_str |
oai:scielo:S2358-04292014000300123 |
network_acronym_str |
METC-1 |
network_name_str |
MedicalExpress (São Paulo. Online) |
repository_id_str |
|
spelling |
Effectiveness of sperm washing by discontinuous density gradient centrifugation to remove antibodies bound to the sperm membraneAntibodiesspermsperm washingimmunobead testmale infertility. OBJECTIVE: We evaluated the effectiveness of sperm washing using the discontinuous two-layer density gradient centrifugation method to remove antisperm antibodies attached to the sperm surface. METHOD: We prospectively enrolled sixty-six men with unexplained infertility who were seeking evaluation. Each patient delivered one semen specimen for the study. We determined antisperm antibody levels using the direct immunobeads test. Specimens were classified into two groups according to the pre-washing levels of antibody-bound spermatozoa: group 1 (low antisperm antibodies levels, immunobeads test < 20%; n = 54), and group 2 (high antisperm antibodies levels, immunobeads test > 20%; n = 12). Sperm washing was carried out using the discontinuous two-layer colloidal density gradient centrifugation method. Pre- and post-wash levels of antisperm antibodies were compared in the groups. RESULTS: The pre- and post-wash percentage of spermatozoa with antisperm antibodies attached to their surface was 11% and 6.5% in group 1 (mean difference = 40%; p < 0.01), and 30% and 19.5% in group 2 (mean difference = 36.8%, p = 0.02), respectively. The effectiveness of density gradient centrifugation in removing antisperm antibodies was not different between the groups, but individual variations from - 52.3% to -3.9% were observed. CONCLUSIONS: Sperm washing by density gradient centrifugation is an overall effective method to remove antibodies bound to sperm membranes, regardless of the levels of antisperm antibodies in the neat semen. Due to an inter-individual variation in the effectiveness of the method, we recommend that each patient be tested before applying sperm washing by density gradient centrifugation in intrauterine insemination.Mavera Edições Técnicas e Científicas Ltda2014-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2358-04292014000300123MedicalExpress v.1 n.3 2014reponame:MedicalExpress (São Paulo. Online)instname:Mavera Edições Científicas e Técnicas Ltda-MEinstacron:METC10.5935/MedicalExpress.2014.03.05info:eu-repo/semantics/openAccessSchneider,DanielleFeijo,CinthiaVerza Jr,SidneyEsteves,Sandroeng2016-06-06T00:00:00Zoai:scielo:S2358-04292014000300123Revistahttp://www.medicalexpress.net.brhttps://old.scielo.br/oai/scielo-oai.php||medicalexpress@me.net.br2358-04292318-8111opendoar:2016-06-06T00:00MedicalExpress (São Paulo. Online) - Mavera Edições Científicas e Técnicas Ltda-MEfalse |
dc.title.none.fl_str_mv |
Effectiveness of sperm washing by discontinuous density gradient centrifugation to remove antibodies bound to the sperm membrane |
title |
Effectiveness of sperm washing by discontinuous density gradient centrifugation to remove antibodies bound to the sperm membrane |
spellingShingle |
Effectiveness of sperm washing by discontinuous density gradient centrifugation to remove antibodies bound to the sperm membrane Schneider,Danielle Antibodies sperm sperm washing immunobead test male infertility. |
title_short |
Effectiveness of sperm washing by discontinuous density gradient centrifugation to remove antibodies bound to the sperm membrane |
title_full |
Effectiveness of sperm washing by discontinuous density gradient centrifugation to remove antibodies bound to the sperm membrane |
title_fullStr |
Effectiveness of sperm washing by discontinuous density gradient centrifugation to remove antibodies bound to the sperm membrane |
title_full_unstemmed |
Effectiveness of sperm washing by discontinuous density gradient centrifugation to remove antibodies bound to the sperm membrane |
title_sort |
Effectiveness of sperm washing by discontinuous density gradient centrifugation to remove antibodies bound to the sperm membrane |
author |
Schneider,Danielle |
author_facet |
Schneider,Danielle Feijo,Cinthia Verza Jr,Sidney Esteves,Sandro |
author_role |
author |
author2 |
Feijo,Cinthia Verza Jr,Sidney Esteves,Sandro |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Schneider,Danielle Feijo,Cinthia Verza Jr,Sidney Esteves,Sandro |
dc.subject.por.fl_str_mv |
Antibodies sperm sperm washing immunobead test male infertility. |
topic |
Antibodies sperm sperm washing immunobead test male infertility. |
description |
OBJECTIVE: We evaluated the effectiveness of sperm washing using the discontinuous two-layer density gradient centrifugation method to remove antisperm antibodies attached to the sperm surface. METHOD: We prospectively enrolled sixty-six men with unexplained infertility who were seeking evaluation. Each patient delivered one semen specimen for the study. We determined antisperm antibody levels using the direct immunobeads test. Specimens were classified into two groups according to the pre-washing levels of antibody-bound spermatozoa: group 1 (low antisperm antibodies levels, immunobeads test < 20%; n = 54), and group 2 (high antisperm antibodies levels, immunobeads test > 20%; n = 12). Sperm washing was carried out using the discontinuous two-layer colloidal density gradient centrifugation method. Pre- and post-wash levels of antisperm antibodies were compared in the groups. RESULTS: The pre- and post-wash percentage of spermatozoa with antisperm antibodies attached to their surface was 11% and 6.5% in group 1 (mean difference = 40%; p < 0.01), and 30% and 19.5% in group 2 (mean difference = 36.8%, p = 0.02), respectively. The effectiveness of density gradient centrifugation in removing antisperm antibodies was not different between the groups, but individual variations from - 52.3% to -3.9% were observed. CONCLUSIONS: Sperm washing by density gradient centrifugation is an overall effective method to remove antibodies bound to sperm membranes, regardless of the levels of antisperm antibodies in the neat semen. Due to an inter-individual variation in the effectiveness of the method, we recommend that each patient be tested before applying sperm washing by density gradient centrifugation in intrauterine insemination. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2358-04292014000300123 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2358-04292014000300123 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.5935/MedicalExpress.2014.03.05 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Mavera Edições Técnicas e Científicas Ltda |
publisher.none.fl_str_mv |
Mavera Edições Técnicas e Científicas Ltda |
dc.source.none.fl_str_mv |
MedicalExpress v.1 n.3 2014 reponame:MedicalExpress (São Paulo. Online) instname:Mavera Edições Científicas e Técnicas Ltda-ME instacron:METC |
instname_str |
Mavera Edições Científicas e Técnicas Ltda-ME |
instacron_str |
METC |
institution |
METC |
reponame_str |
MedicalExpress (São Paulo. Online) |
collection |
MedicalExpress (São Paulo. Online) |
repository.name.fl_str_mv |
MedicalExpress (São Paulo. Online) - Mavera Edições Científicas e Técnicas Ltda-ME |
repository.mail.fl_str_mv |
||medicalexpress@me.net.br |
_version_ |
1754734596568645632 |