Translocation of Klebsiella sp. in mice fed an enteral diet containing prebiotics

• Autor(a) principal: Ferreira da SILVA, Daniele
• Data de Publicação: 2023
• Outros Autores: da Silveira MEZÊNCIO, José Mário, Dantas VANETTI, Maria Cristina, CECON, Paulo Roberto, Loureiro dos SANTOS, Matheus
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Revista de Nutrição
• Texto Completo: https://periodicos.puc-campinas.edu.br/nutricao/article/view/9474
• topic: Inulin; Klebsiella sp; Enteral nutrition; Bacterial translocation; Inulina; Klebsiella sp; Nutrição enteral; Translocação bacteriana

ObjectiveThis work aimed to evaluate the effect of fructooligosaccharide and inulin added to an enteral diet on the translocation of Klebsiella sp. in mice. MethodsFour- to six-week–old Swiss albino mice were divided into nine groups and fed enteral diets containing different combinations of fructooligosaccharide, inulin, antibiotic and corticoid, inoculated or not with Klebsiella pneumoniae. On day 5, the animals of four groups were fed an enteral diet contaminated with approximately 1010CFU/g of K. pneumoniae. At defined times, two animals of each group were sacrificed and their organs (spleen, heart, liver, lungs, and kidneys) were aseptically collected, weighed, and analyzed for the presence of typical Klebsiella sp. colonies. ResultsA higher number of CFU/g of Klebsiella was detected in the organs of the animals in the immune-suppressed group fed the diet contaminated with K. pneumoniae and without prebiotics. Animals fed the diet enriched with fructooligosaccharide and inulin, at a concentration of 15.3mg/g of body weight, had a shorter period of Klebsiella sp. translocation, compared with those not fed prebiotics in the diet. ConclusionThe addition of fructooligosacharide and inulin in enterais diets at a concentration of 15.3mg/g of body weight resulted in the reduction of translocation of Klebsiella for spleen, heart, liver, lung and kidneys of mice that had received the diet contaminated associated or not with antibiotic and imunodepressor drug.