Translocation of Klebsiella sp. in mice fed an enteral diet containing prebiotics
Autor(a) principal: | |
---|---|
Data de Publicação: | 2009 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista de Nutrição |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-52732009000200005 |
Resumo: | OBJECTIVE: This work aimed to evaluate the effect of fructooligosaccharide and inulin added to an enteral diet on the translocation of Klebsiella sp. in mice. METHODS: Four- to six-week-old Swiss albino mice were divided into nine groups and fed enteral diets containing different combinations of fructooligosaccharide, inulin, antibiotic and corticoid, inoculated or not with Klebsiella pneumoniae. On day 5, the animals of four groups were fed an enteral diet contaminated with approximately 10(10)CFU/g of K. pneumoniae. At defined times, two animals of each group were sacrificed and their organs (spleen, heart, liver, lungs, and kidneys) were aseptically collected, weighed, and analyzed for the presence of typical Klebsiella sp. colonies. RESULTS: A higher number of CFU/g of Klebsiella was detected in the organs of the animals in the immune-suppressed group fed the diet contaminated with K. pneumoniae and without prebiotics. Animals fed the diet enriched with fructooligosaccharide and inulin, at a concentration of 15.3mg/g of body weight, had a shorter period of Klebsiella sp. translocation, compared with those not fed prebiotics in the diet. CONCLUSION: The addition of fructooligosacharide and inulin in enterais diets at a concentration of 15.3mg/g of body weight resulted in the reduction of translocation of Klebsiella for spleen, heart, liver, lung and kidneys of mice that had received the diet contaminated associated or not with antibiotic and imunodepressor drug. |
id |
PUC_CAMP-2_1e1f7cc817b4e58dc0a1f49015d3d96c |
---|---|
oai_identifier_str |
oai:scielo:S1415-52732009000200005 |
network_acronym_str |
PUC_CAMP-2 |
network_name_str |
Revista de Nutrição |
repository_id_str |
|
spelling |
Translocation of Klebsiella sp. in mice fed an enteral diet containing prebioticsInulinKlebsiella sp.Enteral nutritionBacterial translocationOBJECTIVE: This work aimed to evaluate the effect of fructooligosaccharide and inulin added to an enteral diet on the translocation of Klebsiella sp. in mice. METHODS: Four- to six-week-old Swiss albino mice were divided into nine groups and fed enteral diets containing different combinations of fructooligosaccharide, inulin, antibiotic and corticoid, inoculated or not with Klebsiella pneumoniae. On day 5, the animals of four groups were fed an enteral diet contaminated with approximately 10(10)CFU/g of K. pneumoniae. At defined times, two animals of each group were sacrificed and their organs (spleen, heart, liver, lungs, and kidneys) were aseptically collected, weighed, and analyzed for the presence of typical Klebsiella sp. colonies. RESULTS: A higher number of CFU/g of Klebsiella was detected in the organs of the animals in the immune-suppressed group fed the diet contaminated with K. pneumoniae and without prebiotics. Animals fed the diet enriched with fructooligosaccharide and inulin, at a concentration of 15.3mg/g of body weight, had a shorter period of Klebsiella sp. translocation, compared with those not fed prebiotics in the diet. CONCLUSION: The addition of fructooligosacharide and inulin in enterais diets at a concentration of 15.3mg/g of body weight resulted in the reduction of translocation of Klebsiella for spleen, heart, liver, lung and kidneys of mice that had received the diet contaminated associated or not with antibiotic and imunodepressor drug.Pontifícia Universidade Católica de Campinas2009-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-52732009000200005Revista de Nutrição v.22 n.2 2009reponame:Revista de Nutriçãoinstname:Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)instacron:PUC_CAMP10.1590/S1415-52732009000200005info:eu-repo/semantics/openAccessSilva,Daniele Ferreira daMezêncio,José Mário da SilveiraVanetti,Maria Cristina DantasCecon,Paulo RobertoSantos,Matheus Loureiro doseng2009-06-25T00:00:00Zoai:scielo:S1415-52732009000200005Revistahttp://www.scielo.br/rnONGhttps://old.scielo.br/oai/scielo-oai.php||sbi.submissionrn@puc-campinas.edu.br1678-98651415-5273opendoar:2009-06-25T00:00Revista de Nutrição - Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS)false |
dc.title.none.fl_str_mv |
Translocation of Klebsiella sp. in mice fed an enteral diet containing prebiotics |
title |
Translocation of Klebsiella sp. in mice fed an enteral diet containing prebiotics |
spellingShingle |
Translocation of Klebsiella sp. in mice fed an enteral diet containing prebiotics Silva,Daniele Ferreira da Inulin Klebsiella sp. Enteral nutrition Bacterial translocation |
title_short |
Translocation of Klebsiella sp. in mice fed an enteral diet containing prebiotics |
title_full |
Translocation of Klebsiella sp. in mice fed an enteral diet containing prebiotics |
title_fullStr |
Translocation of Klebsiella sp. in mice fed an enteral diet containing prebiotics |
title_full_unstemmed |
Translocation of Klebsiella sp. in mice fed an enteral diet containing prebiotics |
title_sort |
Translocation of Klebsiella sp. in mice fed an enteral diet containing prebiotics |
author |
Silva,Daniele Ferreira da |
author_facet |
Silva,Daniele Ferreira da Mezêncio,José Mário da Silveira Vanetti,Maria Cristina Dantas Cecon,Paulo Roberto Santos,Matheus Loureiro dos |
author_role |
author |
author2 |
Mezêncio,José Mário da Silveira Vanetti,Maria Cristina Dantas Cecon,Paulo Roberto Santos,Matheus Loureiro dos |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Silva,Daniele Ferreira da Mezêncio,José Mário da Silveira Vanetti,Maria Cristina Dantas Cecon,Paulo Roberto Santos,Matheus Loureiro dos |
dc.subject.por.fl_str_mv |
Inulin Klebsiella sp. Enteral nutrition Bacterial translocation |
topic |
Inulin Klebsiella sp. Enteral nutrition Bacterial translocation |
description |
OBJECTIVE: This work aimed to evaluate the effect of fructooligosaccharide and inulin added to an enteral diet on the translocation of Klebsiella sp. in mice. METHODS: Four- to six-week-old Swiss albino mice were divided into nine groups and fed enteral diets containing different combinations of fructooligosaccharide, inulin, antibiotic and corticoid, inoculated or not with Klebsiella pneumoniae. On day 5, the animals of four groups were fed an enteral diet contaminated with approximately 10(10)CFU/g of K. pneumoniae. At defined times, two animals of each group were sacrificed and their organs (spleen, heart, liver, lungs, and kidneys) were aseptically collected, weighed, and analyzed for the presence of typical Klebsiella sp. colonies. RESULTS: A higher number of CFU/g of Klebsiella was detected in the organs of the animals in the immune-suppressed group fed the diet contaminated with K. pneumoniae and without prebiotics. Animals fed the diet enriched with fructooligosaccharide and inulin, at a concentration of 15.3mg/g of body weight, had a shorter period of Klebsiella sp. translocation, compared with those not fed prebiotics in the diet. CONCLUSION: The addition of fructooligosacharide and inulin in enterais diets at a concentration of 15.3mg/g of body weight resulted in the reduction of translocation of Klebsiella for spleen, heart, liver, lung and kidneys of mice that had received the diet contaminated associated or not with antibiotic and imunodepressor drug. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-52732009000200005 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-52732009000200005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-52732009000200005 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Pontifícia Universidade Católica de Campinas |
publisher.none.fl_str_mv |
Pontifícia Universidade Católica de Campinas |
dc.source.none.fl_str_mv |
Revista de Nutrição v.22 n.2 2009 reponame:Revista de Nutrição instname:Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS) instacron:PUC_CAMP |
instname_str |
Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS) |
instacron_str |
PUC_CAMP |
institution |
PUC_CAMP |
reponame_str |
Revista de Nutrição |
collection |
Revista de Nutrição |
repository.name.fl_str_mv |
Revista de Nutrição - Pontifícia Universidade Católica de Campinas (PUC-CAMPINAS) |
repository.mail.fl_str_mv |
||sbi.submissionrn@puc-campinas.edu.br |
_version_ |
1752128817361584128 |