Comparative analysis of microbial reduction using photodynamic therapy in bovine teeth infected with Enterococcus faecalis – an in vitro study

• Autor(a) principal: Zechin, Janaina Guzzo
• Data de Publicação: 2016
• Outros Autores: Steier, Liviu, Rossi-Fedele, Giampiero, Gründling, Grasiela Longhi, Sá, Daniela, Guwzinski, Amanda, Trindade, Alessandra Cesar, Souza, Matheus Albino, Oliveira, Silvia Dias de, Figueiredo, Jose Antonio Poli de
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Revista odonto ciência (Online)
• Texto Completo: https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/15365
• topic: Biofilm; Bovine teeth; Enterococcus faecalis; Photodynamic therapy; Biofilme; Dentes bovinos; Enterococcus faecalis; Terapia fotodinâmica

Objective: To evaluate the use of optic fiber and different pre-irradiation times (PIT) in phototherapy with LED for the root canal disinfection of bovine teeth infected in vitro with Enterococcus faecalis.Methodology: The teeth were incubated for 60 days and divided into six groups according to disinfection protocol: Group 1 – distilled water; Group 2 – 1 minute PIT and no optic fiber; Group 3 – 1 minute PIT and optic fiber; Group 4 – 5 minute PIT and no optic fiber; Group 5 – 5 minute PIT and optic fiber; Group 6 – 2% sodium hypochlorite. Microbiological test (CFUs counting) and scanning electron microscopy (SEM) were performed to evaluate the effectiveness of proposed treatments.Results: SEM analysis of canal wall, in the three thirds, revealed group 6 had the best results, whereas microbiological results revealed that group 6 had the best results. No differences in disinfection of root canals were found when optic fiber was used for photodynamic therapy. Conclusion: Photodynamic therapy should not be used alone in the disinfection of root canals, but it may be valuable as a complement for disinfection performed using different cleaning methods.