An?lise in vivo da aplica??o de ?cido hialur?nico e hidroxiapatita/ ? -tric?lcio fosfato (bone ceramic?) em c?lulas mesenquimais diferenciadas em osteoblastos para regenera??o ?ssea alveolar

• Autor(a) principal: Fulginiti, Roberta Limeira
• Data de Publicação: 2021
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da PUC_RS
• Texto Completo: https://tede2.pucrs.br/tede2/handle/tede/10421
• topic: Engenharia Tecidual; Regenera??o ?ssea; Medicina Regenerativa; Hyaluronic acid; Regenerative Medicine; Tissue Engineering; CIENCIAS DA SAUDE::ODONTOLOGIA

The present research aims to evaluate in vivo a cellular compound for bone tissue engineering containing mesenchymal stem cells of adipose origin (mASCs), concentrated supernatant, hybrid hyaluronic acid (AH-HLW) and hydroxyapatite/?-tricalcium-phosphate (HP/?TCP) as bone substitute. Critical bone defects (144) were performed in the femoral region with 2 mm in diameter (two defects per femur) in 36 Lewis rats. The grafting treatments were divided as follows: I- negative control/only the defect (CT); II- mASCs (OST); III- mASCs + AH (AC); IV- HP/?TCP (H); V- Supernatant (SP); VI- AH-HLW + mASCs+ HP/?TCP (ACH). After 15 and 30 days the rats were euthanized and the defects used for microtomographic (micro-CT) and histomorphometric, RT-PCR (Real-Time Polymerase Chain Reaction) and ELISA (Enzyme- Linked Immunosorbent Assay) analysis. The results for the micro-CT tests reagarding bone volume (%) had greater values for (ACH), (H) and (SP) groups. Regarding histomorphometry, (CT) group had greater area of regenerated bone tissue differing from (ACH) group for 15 days, not presenting statistical differences among all groups for the 30 days period. Regarding RT-PCR, type I collagen (col1A) and osteopontin (OP) presented more expressive values for group (SP) in 15 days. For group (H), the BMP-4 was more expressive and for (OST) group OP and BMP-2 for 30 days. Cytokines were evaluated by quantification in the supernatant and greater expression was observed for groups (ACH) e (H) compared to others. Results indicated that group (ACH) secreted more VEGF, FGF and IGF-1 cytokines in 30-days period. The application of bone grafting techniques using acellular compounds derived from the mASC supernatant culture, and compounds with cells associated with bone substitutes or osteoconductive matrices promising results in tissue engineering.