Mecanismos de morte utilizados pelas c?lulas epiteliais alveolares infectadas com o v?rus sincicial respirat?rio
Main Author: | |
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Publication Date: | 2016 |
Format: | Master thesis |
Language: | por |
Source: | Biblioteca Digital de Teses e Dissertações da PUC_RS |
Download full: | http://tede2.pucrs.br/tede2/handle/tede/8402 |
Summary: | Infection by respiratory syncytial virus (RSV) is the leading cause of childhood hospitalization as well as a major health and economic burden worldwide. RSV causes RTI in adults and lower respiratory tract illness (LRTI), including acute bronchiolitis and pneumonia, in infants and young children. Unfortunately, RSV infection provides only limited immune protection to reinfection, mostly due to inadequate immunological memory, which leads to an exacerbated inflammatory response in the respiratory tract promoting airway damage during virus clearance. Despite extensive research efforts, safe and effective vaccines against RSV are currently unavailable. Nevertheless, a prophylactic strategy based on a humanized neutralizing antibody against RSV is widely used in new born at high-risk, such as preterm infants and those suffering from cardiovascular diseases and immunosuppression. The alveolar epithelial cells are the first line of defense against the virus, most associated with the local inflammation in the disease. The entry of respiratory syncytial virus to alveolar epithelial cells (AECs) is an important step in the infection process involving binding of the RSV G and F protein molecules bound to the surface of the host cell. Infection with respiratory syncytial virus is associated with the epithelial cell death and vigorous inflammation, apoptotic death of infected cells is a mechanism to reduce virus replication. And this study showed infection induced by active respiratory syncytial virus in the epithelial cells of the A549 line showing the pathway of cell death induced by the host cells, our results show the pathway of apoptosis induced from the caspase-3 activation and no dependence production of the production of reactive oxygen species. |
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Jones, Marcus Herberthttp://lattes.cnpq.br/2057491798074403Porto, B?rbara Neryhttp://lattes.cnpq.br/8866217194967253http://lattes.cnpq.br/2958585010230491Ferreira, Gabriela Acevedo2019-01-08T16:03:45Z2016-03-03http://tede2.pucrs.br/tede2/handle/tede/8402Infection by respiratory syncytial virus (RSV) is the leading cause of childhood hospitalization as well as a major health and economic burden worldwide. RSV causes RTI in adults and lower respiratory tract illness (LRTI), including acute bronchiolitis and pneumonia, in infants and young children. Unfortunately, RSV infection provides only limited immune protection to reinfection, mostly due to inadequate immunological memory, which leads to an exacerbated inflammatory response in the respiratory tract promoting airway damage during virus clearance. Despite extensive research efforts, safe and effective vaccines against RSV are currently unavailable. Nevertheless, a prophylactic strategy based on a humanized neutralizing antibody against RSV is widely used in new born at high-risk, such as preterm infants and those suffering from cardiovascular diseases and immunosuppression. The alveolar epithelial cells are the first line of defense against the virus, most associated with the local inflammation in the disease. The entry of respiratory syncytial virus to alveolar epithelial cells (AECs) is an important step in the infection process involving binding of the RSV G and F protein molecules bound to the surface of the host cell. Infection with respiratory syncytial virus is associated with the epithelial cell death and vigorous inflammation, apoptotic death of infected cells is a mechanism to reduce virus replication. And this study showed infection induced by active respiratory syncytial virus in the epithelial cells of the A549 line showing the pathway of cell death induced by the host cells, our results show the pathway of apoptosis induced from the caspase-3 activation and no dependence production of the production of reactive oxygen species.A infec??o pelo v?rus sincicial respirat?rio (VSR) ? a principal causa de hospitaliza??o infantil, bem como um grande problema de sa?de e dos custos econ?micos no mundo inteiro. VSR causa infec??o do trato respirat?rio (RTI) em adultos e doen?a do trato respirat?rio inferior (ITRI), incluindo bronquiolite aguda e pneumonia, em crian?as e crian?as jovens. Infelizmente, a infec??o por RSV fornece uma prote??o imune limitada ? uma reinfec??o, principalmente devido a mem?ria imunol?gica insuficiente, o que conduz a uma resposta inflamat?ria exacerbada no trato respirat?rio promovendo dano nas vias a?reas durante a libera??o do v?rus. Com todos os estudos j? feitos, vacinas seguras e eficazes contra RSV est?o atualmente indispon?veis. Mesmo assim, uma estrat?gia profil?tica com base em um anticorpo humanizado neutralizante contra RSV ? amplamente utilizado em rec?m-nascidos de alto risco, tais como rec?m-nascidos prematuros e aqueles que sofrem de doen?as cardiovascular e imunossupress?o. As c?lulas epiteliais alveolares s?o a primeira linha de defesa contra o v?rus, sendo o local mais associado com a inflama??o na doen?a. A entrada do v?rus sincicial respirat?rio para as c?lulas epiteliais alveolares (CEAs) ? um passo importante para o processo de infec??o, envolvendo a liga??o das prote?nas G e F do VSR ? mol?culas ligadas na superf?cie da c?lula hospedeira. A infec??o por v?rus sincicial respirat?rio est? associada com a morte celular epitelial e inflama??o vigorosa, a morte apopt?tica das c?lulas infectadas ? um mecanismo para reduzir a replica??o do v?rus. E neste estudo mostramos a infec??o induzida pelo v?rus sincicial respirat?rio ativo em c?lulas epiteliais da linhagem A549, mostrando a via de morte celular induzida pelas c?lulas hospedeiras, nossos resultados mostram a via da apoptose induzida a partir da ativa??o de caspase-3 e n?o depend?ncia da produ??o da produ??o de esp?cies reativas de oxig?nio.Submitted by PPG Medicina e Ci?ncias da Sa?de (medicina-pg@pucrs.br) on 2019-01-02T13:26:24Z No. of bitstreams: 1 GABRIELAACEVEDOFERREIRA.pdf: 1119548 bytes, checksum: 5ab300a71fcbd4f589e5d85bfe9c6677 (MD5)Approved for entry into archive by Caroline Xavier (caroline.xavier@pucrs.br) on 2019-01-08T15:56:41Z (GMT) No. of bitstreams: 1 GABRIELAACEVEDOFERREIRA.pdf: 1119548 bytes, checksum: 5ab300a71fcbd4f589e5d85bfe9c6677 (MD5)Made available in DSpace on 2019-01-08T16:03:45Z (GMT). No. of bitstreams: 1 GABRIELAACEVEDOFERREIRA.pdf: 1119548 bytes, checksum: 5ab300a71fcbd4f589e5d85bfe9c6677 (MD5) Previous issue date: 2016-03-03Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPESapplication/pdfhttp://tede2.pucrs.br:80/tede2/retrieve/173854/DIS_GABRIELA_ACEVEDO_FERREIRA_CONFIDENCIAL.pdf.jpghttps://tede2.pucrs.br/tede2/retrieve/189625/DIS_GABRIELA_ACEVEDO_FERREIRA_COMPLETO.pdf.jpgporPontif?cia Universidade Cat?lica do Rio Grande do SulPrograma de P?s-Gradua??o em Medicina e Ci?ncias da Sa?dePUCRSBrasilEscola de MedicinaInfec??es Respirat?riasV?rus Sinciciais Respirat?riosMorte CelularApoptoseCIENCIAS DA SAUDE::MEDICINAMecanismos de morte utilizados pelas c?lulas epiteliais alveolares infectadas com o v?rus sincicial respirat?rioinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisTrabalho ser? publicado como artigo ou livro60 meses08/01/2024-721401722658532398500500500600-224747486637135387-9693694523087866273590462550136975366info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da PUC_RSinstname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSORIGINALDIS_GABRIELA_ACEVEDO_FERREIRA_COMPLETO.pdfDIS_GABRIELA_ACEVEDO_FERREIRA_COMPLETO.pdfapplication/pdf1119548https://tede2.pucrs.br/tede2/bitstream/tede/8402/5/DIS_GABRIELA_ACEVEDO_FERREIRA_COMPLETO.pdf5ab300a71fcbd4f589e5d85bfe9c6677MD55THUMBNAILDIS_GABRIELA_ACEVEDO_FERREIRA_CONFIDENCIAL.pdf.jpgDIS_GABRIELA_ACEVEDO_FERREIRA_CONFIDENCIAL.pdf.jpgimage/jpeg4129https://tede2.pucrs.br/tede2/bitstream/tede/8402/4/DIS_GABRIELA_ACEVEDO_FERREIRA_CONFIDENCIAL.pdf.jpgb9012a0e92ffa869728cbac4bce4af31MD54DIS_GABRIELA_ACEVEDO_FERREIRA_COMPLETO.pdf.jpgDIS_GABRIELA_ACEVEDO_FERREIRA_COMPLETO.pdf.jpgimage/jpeg5326https://tede2.pucrs.br/tede2/bitstream/tede/8402/7/DIS_GABRIELA_ACEVEDO_FERREIRA_COMPLETO.pdf.jpga6fa51b9e65db5c9e244d91871d39c49MD57TEXTDIS_GABRIELA_ACEVEDO_FERREIRA_CONFIDENCIAL.pdf.txtDIS_GABRIELA_ACEVEDO_FERREIRA_CONFIDENCIAL.pdf.txttext/plain2812https://tede2.pucrs.br/tede2/bitstream/tede/8402/3/DIS_GABRIELA_ACEVEDO_FERREIRA_CONFIDENCIAL.pdf.txtac4b856f83c66d105734c5e5e2a8bcd2MD53DIS_GABRIELA_ACEVEDO_FERREIRA_COMPLETO.pdf.txtDIS_GABRIELA_ACEVEDO_FERREIRA_COMPLETO.pdf.txttext/plain81072https://tede2.pucrs.br/tede2/bitstream/tede/8402/6/DIS_GABRIELA_ACEVEDO_FERREIRA_COMPLETO.pdf.txtc80f49731df10156c01b353bf7b23ae9MD56LICENSElicense.txtlicense.txttext/plain; charset=utf-8590https://tede2.pucrs.br/tede2/bitstream/tede/8402/1/license.txt220e11f2d3ba5354f917c7035aadef24MD51tede/84022024-01-10 12:00:15.81oai:tede2.pucrs.br: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.pucrs.br/tede2/PRIhttps://tede2.pucrs.br/oai/requestbiblioteca.central@pucrs.br||opendoar:2024-01-10T14:00:15Biblioteca Digital de Teses e Dissertações da PUC_RS - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false |
dc.title.por.fl_str_mv |
Mecanismos de morte utilizados pelas c?lulas epiteliais alveolares infectadas com o v?rus sincicial respirat?rio |
title |
Mecanismos de morte utilizados pelas c?lulas epiteliais alveolares infectadas com o v?rus sincicial respirat?rio |
spellingShingle |
Mecanismos de morte utilizados pelas c?lulas epiteliais alveolares infectadas com o v?rus sincicial respirat?rio Ferreira, Gabriela Acevedo Infec??es Respirat?rias V?rus Sinciciais Respirat?rios Morte Celular Apoptose CIENCIAS DA SAUDE::MEDICINA |
title_short |
Mecanismos de morte utilizados pelas c?lulas epiteliais alveolares infectadas com o v?rus sincicial respirat?rio |
title_full |
Mecanismos de morte utilizados pelas c?lulas epiteliais alveolares infectadas com o v?rus sincicial respirat?rio |
title_fullStr |
Mecanismos de morte utilizados pelas c?lulas epiteliais alveolares infectadas com o v?rus sincicial respirat?rio |
title_full_unstemmed |
Mecanismos de morte utilizados pelas c?lulas epiteliais alveolares infectadas com o v?rus sincicial respirat?rio |
title_sort |
Mecanismos de morte utilizados pelas c?lulas epiteliais alveolares infectadas com o v?rus sincicial respirat?rio |
author |
Ferreira, Gabriela Acevedo |
author_facet |
Ferreira, Gabriela Acevedo |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Jones, Marcus Herbert |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/2057491798074403 |
dc.contributor.advisor-co1.fl_str_mv |
Porto, B?rbara Nery |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/8866217194967253 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2958585010230491 |
dc.contributor.author.fl_str_mv |
Ferreira, Gabriela Acevedo |
contributor_str_mv |
Jones, Marcus Herbert Porto, B?rbara Nery |
dc.subject.por.fl_str_mv |
Infec??es Respirat?rias V?rus Sinciciais Respirat?rios Morte Celular Apoptose |
topic |
Infec??es Respirat?rias V?rus Sinciciais Respirat?rios Morte Celular Apoptose CIENCIAS DA SAUDE::MEDICINA |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE::MEDICINA |
description |
Infection by respiratory syncytial virus (RSV) is the leading cause of childhood hospitalization as well as a major health and economic burden worldwide. RSV causes RTI in adults and lower respiratory tract illness (LRTI), including acute bronchiolitis and pneumonia, in infants and young children. Unfortunately, RSV infection provides only limited immune protection to reinfection, mostly due to inadequate immunological memory, which leads to an exacerbated inflammatory response in the respiratory tract promoting airway damage during virus clearance. Despite extensive research efforts, safe and effective vaccines against RSV are currently unavailable. Nevertheless, a prophylactic strategy based on a humanized neutralizing antibody against RSV is widely used in new born at high-risk, such as preterm infants and those suffering from cardiovascular diseases and immunosuppression. The alveolar epithelial cells are the first line of defense against the virus, most associated with the local inflammation in the disease. The entry of respiratory syncytial virus to alveolar epithelial cells (AECs) is an important step in the infection process involving binding of the RSV G and F protein molecules bound to the surface of the host cell. Infection with respiratory syncytial virus is associated with the epithelial cell death and vigorous inflammation, apoptotic death of infected cells is a mechanism to reduce virus replication. And this study showed infection induced by active respiratory syncytial virus in the epithelial cells of the A549 line showing the pathway of cell death induced by the host cells, our results show the pathway of apoptosis induced from the caspase-3 activation and no dependence production of the production of reactive oxygen species. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016-03-03 |
dc.date.accessioned.fl_str_mv |
2019-01-08T16:03:45Z |
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http://tede2.pucrs.br/tede2/handle/tede/8402 |
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http://tede2.pucrs.br/tede2/handle/tede/8402 |
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por |
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por |
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500 500 500 600 |
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Pontif?cia Universidade Cat?lica do Rio Grande do Sul |
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Programa de P?s-Gradua??o em Medicina e Ci?ncias da Sa?de |
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PUCRS |
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Brasil |
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Escola de Medicina |
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Pontif?cia Universidade Cat?lica do Rio Grande do Sul |
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