Análise comparativa in vitro da citotoxicidade dos ácidos hialurônicos de alto e baixo peso molecular em enxertia óssea com e sem hidroxiapatita e β-tricálciofosfato

• Autor(a) principal: Ribeiro, Carlos Augusto Accorsi
• Data de Publicação: 2014
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da PUC_RS
• Texto Completo: http://tede2.pucrs.br/tede2/handle/tede/1259
• topic: ODONTOLOGIA; IMPLANTODONTIA; ENGENHARIA TECIDUAL; ÁCIDO HIALURÔNICO; CITOTOXINAS; CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA

The reposition of a lost osseous structure is challenging in oral rehabilitations involving osseointegrated implants. Tissue engineering techniques aiming reposition of lost calcified tissues has evolved in the last years as an alternative to the autologous bone graft, without the need of a donor site. Among the required techniques and biomaterials, the hyaluronic acid (HA) presents itself as promising alternative for tissue engineering. HA is present in the extracellular matrix of all living tissues, and also allows modifications in its structure, serving as a carrier for several compounds utilized in bone repair. Depending on its molecular weight, studies show that different cytotoxic effects might be observed. Thus, the present study evaluated, in vitro, the cytotoxicity of high and low molecular weight HA on NIH-3T3 cells, following the ISO 10993-12 test standards for cytotoxicity analysis. A MTT test was conducted with the following groups: (G1) cells + high molecular weight hyaluronic acid; (G2) cells + low molecular weight hyaluronic acid; (G3) cells + high molecular weight hyaluronic acid + hydroxyapatite; (G4) cells + low molecular weight hyaluronic acid + hydroxyapatite; (G5) cells (positive control); (G6) cells+ hypochlorite (negative control). The results show that most groups presented higher cellular viability when compared to the negative control and lower than the positive control group (p<0.05). Group G1 presented no statistical difference when compared to the positive control (G5) (p<0.05), indicating that high molecular weight HA might be applicable as a cell carrier for tissue engineering.