An?lise da citotoxicidade de comp?sitos restauradores para t?cnica direta e indireta : estudo in vitro

Detalhes bibliográficos
Autor(a) principal: Mildner, Mariele Din?
Data de Publicação: 2015
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da PUC_RS
Texto Completo: http://tede2.pucrs.br/tede2/handle/tede/6298
Resumo: An appropriate monomer conversion of composite resins is very important to improve your biocompatibility. The aim of this study was to evaluate additional polymerization methods of dental resins cytotoxicity. A total of 72 samples were prepared from the following materials: Filtek Z350XT? (3M/ESPE, St Paul, MN, EUA), Sinfony (3M/ESPE, St Paul, MN, EUA), Lava Ultimate (3M/ESPE, St Paul, MN, EUA). The used color was A2 for all composite resins. To Filtek Z350XT and Sinfony a Teflon mould (3x2mm) was used. From Lava Ultimate block 1,6mm cubes was cutting with diamond discs and digital pachymeter. Samples was divided in 4 groups: FK Group: 20s LED polymerization, S Group: Sinfony with light and vacuum additional polymerization, L Group: Lava Ultimate, FPA Group: Filtek Z350XT with light and vacuum additional polymerization. Evaluate specimens was stored in DMEM medium for 24, 48 and 72 hours respectively. Thereafter, culture medium was in contact with NIH/3T3 fibroblasts. MTT test determined cellular viability. Statistical analysis realized by Levene and Kolmogorov-Smirnov tests (?=0,05). The S group show the lower cytotoxicity. Additional polymerization of FK group wasn?t efficient to cellular viability. Sinfony presented minor cytotoxicity among all tested materials. Light and vacuum additional polymerization of Filtek Z350XT don?t improve your cytotoxicity results when compared with direct polymerization of the same dental composite.
id P_RS_fdee3b75a6e957d72bc71fd7c5ef5bb9
oai_identifier_str oai:tede2.pucrs.br:tede/6298
network_acronym_str P_RS
network_name_str Biblioteca Digital de Teses e Dissertações da PUC_RS
repository_id_str
spelling Oshima, Hugo Mitsuo Silva603.488.011-49http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4760776E1962.763.390-91http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4950291Z6Mildner, Mariele Din?2015-09-04T19:35:28Z2015-03-24http://tede2.pucrs.br/tede2/handle/tede/6298An appropriate monomer conversion of composite resins is very important to improve your biocompatibility. The aim of this study was to evaluate additional polymerization methods of dental resins cytotoxicity. A total of 72 samples were prepared from the following materials: Filtek Z350XT? (3M/ESPE, St Paul, MN, EUA), Sinfony (3M/ESPE, St Paul, MN, EUA), Lava Ultimate (3M/ESPE, St Paul, MN, EUA). The used color was A2 for all composite resins. To Filtek Z350XT and Sinfony a Teflon mould (3x2mm) was used. From Lava Ultimate block 1,6mm cubes was cutting with diamond discs and digital pachymeter. Samples was divided in 4 groups: FK Group: 20s LED polymerization, S Group: Sinfony with light and vacuum additional polymerization, L Group: Lava Ultimate, FPA Group: Filtek Z350XT with light and vacuum additional polymerization. Evaluate specimens was stored in DMEM medium for 24, 48 and 72 hours respectively. Thereafter, culture medium was in contact with NIH/3T3 fibroblasts. MTT test determined cellular viability. Statistical analysis realized by Levene and Kolmogorov-Smirnov tests (?=0,05). The S group show the lower cytotoxicity. Additional polymerization of FK group wasn?t efficient to cellular viability. Sinfony presented minor cytotoxicity among all tested materials. Light and vacuum additional polymerization of Filtek Z350XT don?t improve your cytotoxicity results when compared with direct polymerization of the same dental composite.Um satisfat?rio grau de convers?o do mon?mero ? importante para melhorar a biocompatibilidade das resinas compostas. O objetivo deste estudo foi avaliar o efeito dos m?todos de polimeriza??o adicional na citotoxicidade de diferentes comp?sitos restauradores. Para isso foram confeccionadas 18 amostras de cada uma das seguintes resinas testadas: Filtek Z350XT? (3M/ESPE, St Paul, MN, EUA), Sinfony (3M/ESPE, St Paul, MN, EUA), Lava Ultimate (3M/ESPE, St Paul, MN, EUA). A cor usada em todas as reinas foi a A2 de corpo. Para as resinas Filtek Z350XT e Sinfony utilizaram-se moldes de politetrafluoretileno de 3x2mm. Para a resina Lava Ultimate foram cortados do bloco cubos com 1,6mm de face usando um disco diamantado e paqu?metro digital. As amostras foram divididas em 4 grupos: Grupo FK: Filtek Z350XT polimerizada por 20s com LED, Grupo S: Sinfony com polimeriza??o adicional com luz e v?cuo, Grupo L: Lava Ultimate, Grupo FPA: Filtek Z350XT com polimeriza??o adicional com luz e v?cuo. As esp?cies avaliadas estiveram em contato com meio DMEM por um per?odo de incuba??o de 24, 48 e 72 horas respectivamente. Ap?s, o meio de cultura foi colocado em contato com as c?lulas de fibroblastos NIH/3T3. Teste de MTT foi realizado para an?lise da viabilidade celular. Testes de Levene e Kolmogorov-Smirnov (?=0,05) foram utilizados para an?lise estat?stica. O grupo S apresentou-se com a citotoxicidade mais baixa. J? a polimeriza??o adicional no grupo FK n?o mostrou-se eficiente para a viabilidade celular. A resina Sinfony apresentou a menor citotoxicidade dentre os tr?s materiais analisados. Uma polimeriza??o adicional por luz e v?cuo na resina Filtek Z350XT n?o melhora seu resultado para citotoxicidade quando comparado a polimeriza??o direta da mesma.Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2015-09-04T19:35:28Z No. of bitstreams: 1 474592 - Texto Completo.pdf: 757600 bytes, checksum: b10e64c161ec1e67c4e8d9aa24a580ae (MD5)Made available in DSpace on 2015-09-04T19:35:28Z (GMT). No. of bitstreams: 1 474592 - Texto Completo.pdf: 757600 bytes, checksum: b10e64c161ec1e67c4e8d9aa24a580ae (MD5) Previous issue date: 2015-03-24Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPESapplication/pdfhttp://tede2.pucrs.br:80/tede2/retrieve/163292/474592%20-%20Texto%20Completo.pdf.jpgporPontif?cia Universidade Cat?lica do Rio Grande do SulPrograma de P?s-Gradua??o em OdontologiaPUCRSBrasilFaculdade de OdontologiaODONTOLOGIAMATERIAIS DENT?RIOSRESINAS COMPOSTASPOLIMERIZA??OCIENCIAS DA SAUDE::ODONTOLOGIAAn?lise da citotoxicidade de comp?sitos restauradores para t?cnica direta e indireta : estudo in vitroinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis-80965548187336651646006006006004673435736271820140-20704984698792443492075167498588264571info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da PUC_RSinstname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSTHUMBNAIL474592 - Texto Completo.pdf.jpg474592 - Texto Completo.pdf.jpgimage/jpeg3273http://tede2.pucrs.br/tede2/bitstream/tede/6298/4/474592+-+Texto+Completo.pdf.jpga2d65f598934ca08c44c035016c10fa0MD54TEXT474592 - Texto Completo.pdf.txt474592 - Texto Completo.pdf.txttext/plain69032http://tede2.pucrs.br/tede2/bitstream/tede/6298/3/474592+-+Texto+Completo.pdf.txt619e46125433f726081f851d46ab46ecMD53ORIGINAL474592 - Texto Completo.pdf474592 - Texto Completo.pdfapplication/pdf757600http://tede2.pucrs.br/tede2/bitstream/tede/6298/2/474592+-+Texto+Completo.pdfb10e64c161ec1e67c4e8d9aa24a580aeMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-8610http://tede2.pucrs.br/tede2/bitstream/tede/6298/1/license.txt5a9d6006225b368ef605ba16b4f6d1beMD51tede/62982015-09-29 08:32:58.379oai:tede2.pucrs.br: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.pucrs.br/tede2/PRIhttps://tede2.pucrs.br/oai/requestbiblioteca.central@pucrs.br||opendoar:2015-09-29T11:32:58Biblioteca Digital de Teses e Dissertações da PUC_RS - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false
dc.title.por.fl_str_mv An?lise da citotoxicidade de comp?sitos restauradores para t?cnica direta e indireta : estudo in vitro
title An?lise da citotoxicidade de comp?sitos restauradores para t?cnica direta e indireta : estudo in vitro
spellingShingle An?lise da citotoxicidade de comp?sitos restauradores para t?cnica direta e indireta : estudo in vitro
Mildner, Mariele Din?
ODONTOLOGIA
MATERIAIS DENT?RIOS
RESINAS COMPOSTAS
POLIMERIZA??O
CIENCIAS DA SAUDE::ODONTOLOGIA
title_short An?lise da citotoxicidade de comp?sitos restauradores para t?cnica direta e indireta : estudo in vitro
title_full An?lise da citotoxicidade de comp?sitos restauradores para t?cnica direta e indireta : estudo in vitro
title_fullStr An?lise da citotoxicidade de comp?sitos restauradores para t?cnica direta e indireta : estudo in vitro
title_full_unstemmed An?lise da citotoxicidade de comp?sitos restauradores para t?cnica direta e indireta : estudo in vitro
title_sort An?lise da citotoxicidade de comp?sitos restauradores para t?cnica direta e indireta : estudo in vitro
author Mildner, Mariele Din?
author_facet Mildner, Mariele Din?
author_role author
dc.contributor.advisor1.fl_str_mv Oshima, Hugo Mitsuo Silva
dc.contributor.advisor1ID.fl_str_mv 603.488.011-49
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4760776E1
dc.contributor.authorID.fl_str_mv 962.763.390-91
dc.contributor.authorLattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4950291Z6
dc.contributor.author.fl_str_mv Mildner, Mariele Din?
contributor_str_mv Oshima, Hugo Mitsuo Silva
dc.subject.por.fl_str_mv ODONTOLOGIA
MATERIAIS DENT?RIOS
RESINAS COMPOSTAS
POLIMERIZA??O
topic ODONTOLOGIA
MATERIAIS DENT?RIOS
RESINAS COMPOSTAS
POLIMERIZA??O
CIENCIAS DA SAUDE::ODONTOLOGIA
dc.subject.cnpq.fl_str_mv CIENCIAS DA SAUDE::ODONTOLOGIA
description An appropriate monomer conversion of composite resins is very important to improve your biocompatibility. The aim of this study was to evaluate additional polymerization methods of dental resins cytotoxicity. A total of 72 samples were prepared from the following materials: Filtek Z350XT? (3M/ESPE, St Paul, MN, EUA), Sinfony (3M/ESPE, St Paul, MN, EUA), Lava Ultimate (3M/ESPE, St Paul, MN, EUA). The used color was A2 for all composite resins. To Filtek Z350XT and Sinfony a Teflon mould (3x2mm) was used. From Lava Ultimate block 1,6mm cubes was cutting with diamond discs and digital pachymeter. Samples was divided in 4 groups: FK Group: 20s LED polymerization, S Group: Sinfony with light and vacuum additional polymerization, L Group: Lava Ultimate, FPA Group: Filtek Z350XT with light and vacuum additional polymerization. Evaluate specimens was stored in DMEM medium for 24, 48 and 72 hours respectively. Thereafter, culture medium was in contact with NIH/3T3 fibroblasts. MTT test determined cellular viability. Statistical analysis realized by Levene and Kolmogorov-Smirnov tests (?=0,05). The S group show the lower cytotoxicity. Additional polymerization of FK group wasn?t efficient to cellular viability. Sinfony presented minor cytotoxicity among all tested materials. Light and vacuum additional polymerization of Filtek Z350XT don?t improve your cytotoxicity results when compared with direct polymerization of the same dental composite.
publishDate 2015
dc.date.accessioned.fl_str_mv 2015-09-04T19:35:28Z
dc.date.issued.fl_str_mv 2015-03-24
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://tede2.pucrs.br/tede2/handle/tede/6298
url http://tede2.pucrs.br/tede2/handle/tede/6298
dc.language.iso.fl_str_mv por
language por
dc.relation.program.fl_str_mv -8096554818733665164
dc.relation.confidence.fl_str_mv 600
600
600
600
dc.relation.department.fl_str_mv 4673435736271820140
dc.relation.cnpq.fl_str_mv -2070498469879244349
dc.relation.sponsorship.fl_str_mv 2075167498588264571
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pontif?cia Universidade Cat?lica do Rio Grande do Sul
dc.publisher.program.fl_str_mv Programa de P?s-Gradua??o em Odontologia
dc.publisher.initials.fl_str_mv PUCRS
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Faculdade de Odontologia
publisher.none.fl_str_mv Pontif?cia Universidade Cat?lica do Rio Grande do Sul
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS
instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron:PUC_RS
instname_str Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron_str PUC_RS
institution PUC_RS
reponame_str Biblioteca Digital de Teses e Dissertações da PUC_RS
collection Biblioteca Digital de Teses e Dissertações da PUC_RS
bitstream.url.fl_str_mv http://tede2.pucrs.br/tede2/bitstream/tede/6298/4/474592+-+Texto+Completo.pdf.jpg
http://tede2.pucrs.br/tede2/bitstream/tede/6298/3/474592+-+Texto+Completo.pdf.txt
http://tede2.pucrs.br/tede2/bitstream/tede/6298/2/474592+-+Texto+Completo.pdf
http://tede2.pucrs.br/tede2/bitstream/tede/6298/1/license.txt
bitstream.checksum.fl_str_mv a2d65f598934ca08c44c035016c10fa0
619e46125433f726081f851d46ab46ec
b10e64c161ec1e67c4e8d9aa24a580ae
5a9d6006225b368ef605ba16b4f6d1be
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações da PUC_RS - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
repository.mail.fl_str_mv biblioteca.central@pucrs.br||
_version_ 1796793096128692224

Registros relacionados