Synthesis and characterization of biocompatible methacrylated Kefiran hydrogels: towards tissue engineering applications
Main Author: | |
---|---|
Publication Date: | 2021 |
Other Authors: | , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Download full: | http://hdl.handle.net/1822/72167 |
Summary: | Hydrogel application feasibility is still limited mainly due to their low mechanical strength and fragile nature. Therefore, several physical and chemical cross-linking modifications are being used to improve their properties. In this research, methacrylated Kefiran was synthesized by reacting Kefiran with methacrylic anhydride (MA). The developed MA-Kefiran was physicochemically characterized, and its biological properties evaluated by different techniques. Chemical modification of MA-Kefiran was confirmed by 1H-NMR and FTIR and GPC-SEC showed an average Mw of 793 kDa (PDI 1.3). The mechanical data obtained revealed MA-Kefiran to be a pseudoplastic fluid with an extrusion force of 11.21 ± 2.87 N. Moreover, MA-Kefiran 3D cryogels were successfully developed and fully characterized. Through micro-CT and SEM, the scaffolds revealed high porosity (85.53 ± 0.15%) and pore size (33.67 ± 3.13 μm), thick pore walls (11.92 ± 0.44 μm) and a homogeneous structure. Finally, MA-Kefiran revealed to be biocompatible by presenting no hemolytic activity and an improved cellular function of L929 cells observed through the AlamarBlue® assay. By incorporating methacrylate groups in the Kefiran polysaccharide chain, a MA-Kefiran product was developed with remarkable physical, mechanical, and biological properties, resulting in a promising hydrogel to be used in tissue engineering and regenerative medicine applications. |
id |
RCAP_004dec0eeac97a1e0e31686ea577d94c |
---|---|
oai_identifier_str |
oai:repositorium.sdum.uminho.pt:1822/72167 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Synthesis and characterization of biocompatible methacrylated Kefiran hydrogels: towards tissue engineering applicationsBiomedical applicationsChemical cross-linkingHydrogelKefiranMethacrylationScience & TechnologyHydrogel application feasibility is still limited mainly due to their low mechanical strength and fragile nature. Therefore, several physical and chemical cross-linking modifications are being used to improve their properties. In this research, methacrylated Kefiran was synthesized by reacting Kefiran with methacrylic anhydride (MA). The developed MA-Kefiran was physicochemically characterized, and its biological properties evaluated by different techniques. Chemical modification of MA-Kefiran was confirmed by 1H-NMR and FTIR and GPC-SEC showed an average Mw of 793 kDa (PDI 1.3). The mechanical data obtained revealed MA-Kefiran to be a pseudoplastic fluid with an extrusion force of 11.21 ± 2.87 N. Moreover, MA-Kefiran 3D cryogels were successfully developed and fully characterized. Through micro-CT and SEM, the scaffolds revealed high porosity (85.53 ± 0.15%) and pore size (33.67 ± 3.13 μm), thick pore walls (11.92 ± 0.44 μm) and a homogeneous structure. Finally, MA-Kefiran revealed to be biocompatible by presenting no hemolytic activity and an improved cellular function of L929 cells observed through the AlamarBlue® assay. By incorporating methacrylate groups in the Kefiran polysaccharide chain, a MA-Kefiran product was developed with remarkable physical, mechanical, and biological properties, resulting in a promising hydrogel to be used in tissue engineering and regenerative medicine applications.H. Radhouani and C. Goncalveswere supported by the Foundation for Science and Technology (FCT) fromPortugal, with references CEECIND/00111/2017 and SFRH/BPD/94277/2013, respectively. S. Correia and this work were funded by the R&D Project KOAT-Kefiran Exopolysaccharide: Promising Biopolymer for Use in Regenerative Medicine and Tissue Engineering, with reference PTDC/BTMMAT/29760/2017 (POCI-01-0145-FEDER-029760), financed by FCT and co-financed by FEDER and POCI. We also thank Duarte N. Carvalho for input on the schematic representation of the process.MDPIUniversidade do MinhoRadhouani, HajerCorreia, SusanaGonçalves, CristianaReis, R. L.Oliveira, J. M.2021-042021-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/72167engRadhouani H., Correia S., Gonçalves C., Reis R. L., Oliveira J. M. Synthesis and Characterization of Biocompatible Methacrylated Kefiran Hydrogels: Towards Tissue Engineering Applications, Polymers, Vol. 13, Issue 8, pp. 1342, doi:10.3390/polym13081342, 20212073-436010.3390/polym13081342https://www.mdpi.com/2073-4360/13/8/1342info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:34:43Zoai:repositorium.sdum.uminho.pt:1822/72167Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:30:26.751429Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Synthesis and characterization of biocompatible methacrylated Kefiran hydrogels: towards tissue engineering applications |
title |
Synthesis and characterization of biocompatible methacrylated Kefiran hydrogels: towards tissue engineering applications |
spellingShingle |
Synthesis and characterization of biocompatible methacrylated Kefiran hydrogels: towards tissue engineering applications Radhouani, Hajer Biomedical applications Chemical cross-linking Hydrogel Kefiran Methacrylation Science & Technology |
title_short |
Synthesis and characterization of biocompatible methacrylated Kefiran hydrogels: towards tissue engineering applications |
title_full |
Synthesis and characterization of biocompatible methacrylated Kefiran hydrogels: towards tissue engineering applications |
title_fullStr |
Synthesis and characterization of biocompatible methacrylated Kefiran hydrogels: towards tissue engineering applications |
title_full_unstemmed |
Synthesis and characterization of biocompatible methacrylated Kefiran hydrogels: towards tissue engineering applications |
title_sort |
Synthesis and characterization of biocompatible methacrylated Kefiran hydrogels: towards tissue engineering applications |
author |
Radhouani, Hajer |
author_facet |
Radhouani, Hajer Correia, Susana Gonçalves, Cristiana Reis, R. L. Oliveira, J. M. |
author_role |
author |
author2 |
Correia, Susana Gonçalves, Cristiana Reis, R. L. Oliveira, J. M. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Radhouani, Hajer Correia, Susana Gonçalves, Cristiana Reis, R. L. Oliveira, J. M. |
dc.subject.por.fl_str_mv |
Biomedical applications Chemical cross-linking Hydrogel Kefiran Methacrylation Science & Technology |
topic |
Biomedical applications Chemical cross-linking Hydrogel Kefiran Methacrylation Science & Technology |
description |
Hydrogel application feasibility is still limited mainly due to their low mechanical strength and fragile nature. Therefore, several physical and chemical cross-linking modifications are being used to improve their properties. In this research, methacrylated Kefiran was synthesized by reacting Kefiran with methacrylic anhydride (MA). The developed MA-Kefiran was physicochemically characterized, and its biological properties evaluated by different techniques. Chemical modification of MA-Kefiran was confirmed by 1H-NMR and FTIR and GPC-SEC showed an average Mw of 793 kDa (PDI 1.3). The mechanical data obtained revealed MA-Kefiran to be a pseudoplastic fluid with an extrusion force of 11.21 ± 2.87 N. Moreover, MA-Kefiran 3D cryogels were successfully developed and fully characterized. Through micro-CT and SEM, the scaffolds revealed high porosity (85.53 ± 0.15%) and pore size (33.67 ± 3.13 μm), thick pore walls (11.92 ± 0.44 μm) and a homogeneous structure. Finally, MA-Kefiran revealed to be biocompatible by presenting no hemolytic activity and an improved cellular function of L929 cells observed through the AlamarBlue® assay. By incorporating methacrylate groups in the Kefiran polysaccharide chain, a MA-Kefiran product was developed with remarkable physical, mechanical, and biological properties, resulting in a promising hydrogel to be used in tissue engineering and regenerative medicine applications. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-04 2021-04-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/72167 |
url |
http://hdl.handle.net/1822/72167 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Radhouani H., Correia S., Gonçalves C., Reis R. L., Oliveira J. M. Synthesis and Characterization of Biocompatible Methacrylated Kefiran Hydrogels: Towards Tissue Engineering Applications, Polymers, Vol. 13, Issue 8, pp. 1342, doi:10.3390/polym13081342, 2021 2073-4360 10.3390/polym13081342 https://www.mdpi.com/2073-4360/13/8/1342 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799132808622374912 |