Development of conjugated kefiran-chondroitin sulphate cryogels with enhanced properties for biomedical applications
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/85692 |
Resumo: | Hydrogels based on natural polysaccharides can have unique properties and be tailored for several applications, which may be mainly limited by the fragile structure and weak mechanical properties of this type of system. We successfully prepared cryogels made of newly synthesized kefiran exopolysaccharide-chondroitin sulfate (CS) conjugate via carbodiimide-mediated coupling to overcome these drawbacks. The freeze-thawing procedure of cryogel preparation followed by lyophilization is a promising route to fabricate polymer-based scaffolds with countless and valuable biomedical applications. The novel graft macromolecular compound (kefiran-CS conjugate) was characterized through <sup>1</sup>H-NMR and FTIR spectroscopy—which confirmed the structure of the conjugate, differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA)—which mirrored good thermal stability (degradation temperature of about 215 °C) and, finally, gel permeation chromatography–size exclusion chromatography (GPC-SEC)—which proved an increased molecular weight due to chemical coupling of kefiran with CS. At the same time, the corresponding cryogels physically crosslinked after the freeze-thawing procedure were investigated by scanning electron microscopy (SEM), Micro-CT, and dynamic rheology. The results revealed a prevalent contribution of elastic/storage component to the viscoelastic behavior of cryogels in swollen state, a micromorphology with micrometer-sized open pores fully interconnected, and high porosity (ca. 90%) observed for freeze-dried cryogels. Furthermore, the metabolic activity and proliferation of human adipose stem cells (hASCs), when cultured onto the developed kefiran-CS cryogel, was maintained at a satisfactory level over 72 h. Based on the results obtained, it can be inferred that the newly freeze-dried kefiran-CS cryogels possess a host of unique properties that render them highly suitable for use in tissue engineering, regenerative medicine, drug delivery, and other biomedical applications where robust mechanical properties and biocompatibility are crucial. |
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Development of conjugated kefiran-chondroitin sulphate cryogels with enhanced properties for biomedical applicationsBiomedical deviceCarbodiimide-mediated couplingCharacterizationChondroitin sulfateCryogelation techniqueKefiranScaffoldHydrogels based on natural polysaccharides can have unique properties and be tailored for several applications, which may be mainly limited by the fragile structure and weak mechanical properties of this type of system. We successfully prepared cryogels made of newly synthesized kefiran exopolysaccharide-chondroitin sulfate (CS) conjugate via carbodiimide-mediated coupling to overcome these drawbacks. The freeze-thawing procedure of cryogel preparation followed by lyophilization is a promising route to fabricate polymer-based scaffolds with countless and valuable biomedical applications. The novel graft macromolecular compound (kefiran-CS conjugate) was characterized through <sup>1</sup>H-NMR and FTIR spectroscopy—which confirmed the structure of the conjugate, differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA)—which mirrored good thermal stability (degradation temperature of about 215 °C) and, finally, gel permeation chromatography–size exclusion chromatography (GPC-SEC)—which proved an increased molecular weight due to chemical coupling of kefiran with CS. At the same time, the corresponding cryogels physically crosslinked after the freeze-thawing procedure were investigated by scanning electron microscopy (SEM), Micro-CT, and dynamic rheology. The results revealed a prevalent contribution of elastic/storage component to the viscoelastic behavior of cryogels in swollen state, a micromorphology with micrometer-sized open pores fully interconnected, and high porosity (ca. 90%) observed for freeze-dried cryogels. Furthermore, the metabolic activity and proliferation of human adipose stem cells (hASCs), when cultured onto the developed kefiran-CS cryogel, was maintained at a satisfactory level over 72 h. Based on the results obtained, it can be inferred that the newly freeze-dried kefiran-CS cryogels possess a host of unique properties that render them highly suitable for use in tissue engineering, regenerative medicine, drug delivery, and other biomedical applications where robust mechanical properties and biocompatibility are crucial.This research was funded by the Foundation for Science and Technology (FCT) from Portugal, with references CEECIND/00111/2017 and SFRH/BPD/94277/2013, respectively.H.R. and C.G. were supported by the Foundation for Science and Technology (FCT) from Portugal, with references CEECIND/00111/2017 and SFRH/BPD/94277/2013, respectively. The authors would like to express their sincere gratitude to Emanuel Fernandes for his invaluable contribution to this research by performing the STA experiment. The authors thank the financial support provided under the project “HEALTH-UNORTE: Setting-up biobanks and regenerative medicine strategies to boost research in cardiovascular, musculoskeletal, neurological, oncological, immunological and infectious diseases”, reference NORTE-01-0145-FEDER-000039, funded by the Norte Portugal Regional Coordination and Development Commission (CCDR-N), under the NORTE2020 Program.Multidisciplinary Digital Publishing InstituteUniversidade do MinhoRadhouani, HajerGonçalves, CristianaMaia, F. RaquelOliveira, Maria Eduarda Moreira Pinheiro SantosReis, R. L.Oliveira, Joaquim M.2023-06-052023-06-05T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/85692engRadhouani, H.; Gonçalves, C.; Maia, F.R.; Oliveira, E.P.; Reis, R.L.; Oliveira, J.M. Development of Conjugated Kefiran-Chondroitin Sulphate Cryogels with Enhanced Properties for Biomedical Applications. Pharmaceutics 2023, 15, 1662. https://doi.org/10.3390/pharmaceutics150616621999-492310.3390/pharmaceutics15061662https://www.mdpi.com/1999-4923/15/6/1662info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-29T01:20:10ZPortal AgregadorONG |
dc.title.none.fl_str_mv |
Development of conjugated kefiran-chondroitin sulphate cryogels with enhanced properties for biomedical applications |
title |
Development of conjugated kefiran-chondroitin sulphate cryogels with enhanced properties for biomedical applications |
spellingShingle |
Development of conjugated kefiran-chondroitin sulphate cryogels with enhanced properties for biomedical applications Radhouani, Hajer Biomedical device Carbodiimide-mediated coupling Characterization Chondroitin sulfate Cryogelation technique Kefiran Scaffold |
title_short |
Development of conjugated kefiran-chondroitin sulphate cryogels with enhanced properties for biomedical applications |
title_full |
Development of conjugated kefiran-chondroitin sulphate cryogels with enhanced properties for biomedical applications |
title_fullStr |
Development of conjugated kefiran-chondroitin sulphate cryogels with enhanced properties for biomedical applications |
title_full_unstemmed |
Development of conjugated kefiran-chondroitin sulphate cryogels with enhanced properties for biomedical applications |
title_sort |
Development of conjugated kefiran-chondroitin sulphate cryogels with enhanced properties for biomedical applications |
author |
Radhouani, Hajer |
author_facet |
Radhouani, Hajer Gonçalves, Cristiana Maia, F. Raquel Oliveira, Maria Eduarda Moreira Pinheiro Santos Reis, R. L. Oliveira, Joaquim M. |
author_role |
author |
author2 |
Gonçalves, Cristiana Maia, F. Raquel Oliveira, Maria Eduarda Moreira Pinheiro Santos Reis, R. L. Oliveira, Joaquim M. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Radhouani, Hajer Gonçalves, Cristiana Maia, F. Raquel Oliveira, Maria Eduarda Moreira Pinheiro Santos Reis, R. L. Oliveira, Joaquim M. |
dc.subject.por.fl_str_mv |
Biomedical device Carbodiimide-mediated coupling Characterization Chondroitin sulfate Cryogelation technique Kefiran Scaffold |
topic |
Biomedical device Carbodiimide-mediated coupling Characterization Chondroitin sulfate Cryogelation technique Kefiran Scaffold |
description |
Hydrogels based on natural polysaccharides can have unique properties and be tailored for several applications, which may be mainly limited by the fragile structure and weak mechanical properties of this type of system. We successfully prepared cryogels made of newly synthesized kefiran exopolysaccharide-chondroitin sulfate (CS) conjugate via carbodiimide-mediated coupling to overcome these drawbacks. The freeze-thawing procedure of cryogel preparation followed by lyophilization is a promising route to fabricate polymer-based scaffolds with countless and valuable biomedical applications. The novel graft macromolecular compound (kefiran-CS conjugate) was characterized through <sup>1</sup>H-NMR and FTIR spectroscopy—which confirmed the structure of the conjugate, differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA)—which mirrored good thermal stability (degradation temperature of about 215 °C) and, finally, gel permeation chromatography–size exclusion chromatography (GPC-SEC)—which proved an increased molecular weight due to chemical coupling of kefiran with CS. At the same time, the corresponding cryogels physically crosslinked after the freeze-thawing procedure were investigated by scanning electron microscopy (SEM), Micro-CT, and dynamic rheology. The results revealed a prevalent contribution of elastic/storage component to the viscoelastic behavior of cryogels in swollen state, a micromorphology with micrometer-sized open pores fully interconnected, and high porosity (ca. 90%) observed for freeze-dried cryogels. Furthermore, the metabolic activity and proliferation of human adipose stem cells (hASCs), when cultured onto the developed kefiran-CS cryogel, was maintained at a satisfactory level over 72 h. Based on the results obtained, it can be inferred that the newly freeze-dried kefiran-CS cryogels possess a host of unique properties that render them highly suitable for use in tissue engineering, regenerative medicine, drug delivery, and other biomedical applications where robust mechanical properties and biocompatibility are crucial. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-06-05 2023-06-05T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/85692 |
url |
https://hdl.handle.net/1822/85692 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Radhouani, H.; Gonçalves, C.; Maia, F.R.; Oliveira, E.P.; Reis, R.L.; Oliveira, J.M. Development of Conjugated Kefiran-Chondroitin Sulphate Cryogels with Enhanced Properties for Biomedical Applications. Pharmaceutics 2023, 15, 1662. https://doi.org/10.3390/pharmaceutics15061662 1999-4923 10.3390/pharmaceutics15061662 https://www.mdpi.com/1999-4923/15/6/1662 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute |
publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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