New molecular markers for phlebotomine sand flies

• Autor(a) principal: Peixoto, Alexandre Afrânio
• Data de Publicação: 2001
• Outros Autores: Alcobia Gomes, Célia Margarida, de Amoretty, P.R., Lins, R.M.M.A., Meireles-Filho, A.C.A., de Souza, N.A., Kyriacou, C.P.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10400.26/33485
• topic: Genetics; Molecular markers; Lutzomyia; Cacophony gene; Period gene; Psychodidae

Using degenerate-primers PCR we isolated and sequenced fragments from the sand fly Lutzomyia longipalpis homologous to two behavioural genes in Drosophila, cacophony and period. In addition we identified a number of other gene fragments that show homology to genes previously cloned in Drosophila. A codon usage table for L. longipalpis based on these and other genes was calculated. These new molecular markers will be useful in population genetics and evolutionary studies in phlebotomine sand flies and in establishing a preliminary genetic map in these important leishmaniasis vectors.