Moderately acidophilic Mesorhizobia isolated from chickpea (Cicer arietinum L.)
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/1576 |
Resumo: | Aims: Our aim was to evaluate the effect of acid and alkaline pH on chickpea rhizobia, and on chickpea–rhizobia symbiosis. Methods and Results: Forty-seven rhizobia isolates obtained from 12 Portuguese soils were grown at pH 5, 7 and 9. Among these, 26 grew more at pH 5 than at 7, suggesting the existence of acidophiles. All isolates were identified as mesorhizobia by 16S rDNA partial sequence analysis. Molecular phylogeny of isolates based on partial 16S rDNA sequences suggests that pH tolerance might be species related. Further studies were conducted with six isolates, which were able to grow at acid pH. Isolates PT-35 and 64b grow optimally at pH 6–5–7, with a minimal pH range from 5 to 3, and may thus be considered as moderately acidophilic. Both isolates belong to a previously identified putative new Mesorhizobium species, based on 16S rDNA sequence. Conclusions: Two moderately acidophilic mesorhizobia isolated from chickpea were identified (PT-35 and 64b). A positive correlation was found between the symbiotic effectiveness at low pH and the acid tolerance of rhizobia isolates. Significance and Impact of the Study: This is the first report on moderately acidophilic mesorhizobia, and is an important contribution for the development of highly effective inoculants for chickpea in acid soils. |
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Moderately acidophilic Mesorhizobia isolated from chickpea (Cicer arietinum L.)MesorhizobiumacidityAims: Our aim was to evaluate the effect of acid and alkaline pH on chickpea rhizobia, and on chickpea–rhizobia symbiosis. Methods and Results: Forty-seven rhizobia isolates obtained from 12 Portuguese soils were grown at pH 5, 7 and 9. Among these, 26 grew more at pH 5 than at 7, suggesting the existence of acidophiles. All isolates were identified as mesorhizobia by 16S rDNA partial sequence analysis. Molecular phylogeny of isolates based on partial 16S rDNA sequences suggests that pH tolerance might be species related. Further studies were conducted with six isolates, which were able to grow at acid pH. Isolates PT-35 and 64b grow optimally at pH 6–5–7, with a minimal pH range from 5 to 3, and may thus be considered as moderately acidophilic. Both isolates belong to a previously identified putative new Mesorhizobium species, based on 16S rDNA sequence. Conclusions: Two moderately acidophilic mesorhizobia isolated from chickpea were identified (PT-35 and 64b). A positive correlation was found between the symbiotic effectiveness at low pH and the acid tolerance of rhizobia isolates. Significance and Impact of the Study: This is the first report on moderately acidophilic mesorhizobia, and is an important contribution for the development of highly effective inoculants for chickpea in acid soils.2009-04-15T15:42:38Z2009-04-152007-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article8746 bytesapplication/pdfhttp://hdl.handle.net/10174/1576http://hdl.handle.net/10174/1576engpag 168-174Letters in Applied Microbiology244livrendndmlaranjo@uevora.ptismo@uevora.pt371Brígido, ClarisseAlexandre, AnaLaranjo, MartaOliveira, Solangeinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T18:37:16Zoai:dspace.uevora.pt:10174/1576Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:57:26.751497Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Moderately acidophilic Mesorhizobia isolated from chickpea (Cicer arietinum L.) |
title |
Moderately acidophilic Mesorhizobia isolated from chickpea (Cicer arietinum L.) |
spellingShingle |
Moderately acidophilic Mesorhizobia isolated from chickpea (Cicer arietinum L.) Brígido, Clarisse Mesorhizobium acidity |
title_short |
Moderately acidophilic Mesorhizobia isolated from chickpea (Cicer arietinum L.) |
title_full |
Moderately acidophilic Mesorhizobia isolated from chickpea (Cicer arietinum L.) |
title_fullStr |
Moderately acidophilic Mesorhizobia isolated from chickpea (Cicer arietinum L.) |
title_full_unstemmed |
Moderately acidophilic Mesorhizobia isolated from chickpea (Cicer arietinum L.) |
title_sort |
Moderately acidophilic Mesorhizobia isolated from chickpea (Cicer arietinum L.) |
author |
Brígido, Clarisse |
author_facet |
Brígido, Clarisse Alexandre, Ana Laranjo, Marta Oliveira, Solange |
author_role |
author |
author2 |
Alexandre, Ana Laranjo, Marta Oliveira, Solange |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Brígido, Clarisse Alexandre, Ana Laranjo, Marta Oliveira, Solange |
dc.subject.por.fl_str_mv |
Mesorhizobium acidity |
topic |
Mesorhizobium acidity |
description |
Aims: Our aim was to evaluate the effect of acid and alkaline pH on chickpea rhizobia, and on chickpea–rhizobia symbiosis. Methods and Results: Forty-seven rhizobia isolates obtained from 12 Portuguese soils were grown at pH 5, 7 and 9. Among these, 26 grew more at pH 5 than at 7, suggesting the existence of acidophiles. All isolates were identified as mesorhizobia by 16S rDNA partial sequence analysis. Molecular phylogeny of isolates based on partial 16S rDNA sequences suggests that pH tolerance might be species related. Further studies were conducted with six isolates, which were able to grow at acid pH. Isolates PT-35 and 64b grow optimally at pH 6–5–7, with a minimal pH range from 5 to 3, and may thus be considered as moderately acidophilic. Both isolates belong to a previously identified putative new Mesorhizobium species, based on 16S rDNA sequence. Conclusions: Two moderately acidophilic mesorhizobia isolated from chickpea were identified (PT-35 and 64b). A positive correlation was found between the symbiotic effectiveness at low pH and the acid tolerance of rhizobia isolates. Significance and Impact of the Study: This is the first report on moderately acidophilic mesorhizobia, and is an important contribution for the development of highly effective inoculants for chickpea in acid soils. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-01-01T00:00:00Z 2009-04-15T15:42:38Z 2009-04-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/1576 http://hdl.handle.net/10174/1576 |
url |
http://hdl.handle.net/10174/1576 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
pag 168-174 Letters in Applied Microbiology 2 44 livre nd nd mlaranjo@uevora.pt ismo@uevora.pt 371 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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8746 bytes application/pdf |
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