Deformation of red blood cells, air bubbles, and droplets in microfluidic devices: flow visualizations and measurements
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/60126 |
Resumo: | Techniques, such as micropipette aspiration and optical tweezers, are widely used to measure cell mechanical properties, but are generally labor-intensive and time-consuming, typically involving a difficult process of manipulation. In the past two decades, a large number of microfluidic devices have been developed due to the advantages they offer over other techniques, including transparency for direct optical access, lower cost, reduced space and labor, precise control, and easy manipulation of a small volume of blood samples. This review presents recent advances in the development of microfluidic devices to evaluate the mechanical response of individual red blood cells (RBCs) and microbubbles flowing in constriction microchannels. Visualizations and measurements of the deformation of RBCs flowing through hyperbolic, smooth, and sudden-contraction microchannels were evaluated and compared. In particular, we show the potential of using hyperbolic-shaped microchannels to precisely control and assess small changes in RBC deformability in both physiological and pathological situations. Moreover, deformations of air microbubbles and droplets flowing through a microfluidic constriction were also compared with RBCs deformability. |
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Deformation of red blood cells, air bubbles, and droplets in microfluidic devices: flow visualizations and measurementsred blood cellsdeformation indexmicrofluidic devicesair bubblesdropletsblood flowScience & TechnologyTechniques, such as micropipette aspiration and optical tweezers, are widely used to measure cell mechanical properties, but are generally labor-intensive and time-consuming, typically involving a difficult process of manipulation. In the past two decades, a large number of microfluidic devices have been developed due to the advantages they offer over other techniques, including transparency for direct optical access, lower cost, reduced space and labor, precise control, and easy manipulation of a small volume of blood samples. This review presents recent advances in the development of microfluidic devices to evaluate the mechanical response of individual red blood cells (RBCs) and microbubbles flowing in constriction microchannels. Visualizations and measurements of the deformation of RBCs flowing through hyperbolic, smooth, and sudden-contraction microchannels were evaluated and compared. In particular, we show the potential of using hyperbolic-shaped microchannels to precisely control and assess small changes in RBC deformability in both physiological and pathological situations. Moreover, deformations of air microbubbles and droplets flowing through a microfluidic constriction were also compared with RBCs deformability.The authors acknowledge the financial support provided by the project POCI-01-0145 FEDER-016861 (with associated reference PTDC/QEQ-FTT/4287/2014) from FCT (Foundation for Science and Technology) and FEDER. This work was also funded by the ERDF—European Regional Development Fund through the COMPETE Programme (operational programme for competitiveness) and by National Funds through the FCT within projects and UID/EMS/00532/2013, UID/EMS/04077/2013 and the projects POCI-01-0145-FEDER-007043, POCI-01-0145-FEDER-006961, UID/CEC/00319/2013. David Bento, Raquel O. Rodrigues, Diana Pinho, and Vera Faustino, acknowledge, respectively, the PhD scholarships SFRH/BD/91192/2012, SFRH/BD/97658/2013, SFRH/BD/89077/2012, and SFRH/BD/99696/2014, and Rui Lima acknowledge fellowship SFRH/BSAB/135419/2017, all granted by FCT.Multidisciplinary Digital Publishing InstituteUniversidade do MinhoBento, DavidRodrigues, Raquel O.Faustino, VeraPinho, DianaFernandes, Carla S.Pereira, Ana I.Garcia, ValdemarMiranda, João M.Lima, Rui Alberto Madeira Macedo2018-03-272018-03-27T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/60126eng2072-666X10.3390/mi9040151info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:06:14Zoai:repositorium.sdum.uminho.pt:1822/60126Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:56:51.308864Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Deformation of red blood cells, air bubbles, and droplets in microfluidic devices: flow visualizations and measurements |
title |
Deformation of red blood cells, air bubbles, and droplets in microfluidic devices: flow visualizations and measurements |
spellingShingle |
Deformation of red blood cells, air bubbles, and droplets in microfluidic devices: flow visualizations and measurements Bento, David red blood cells deformation index microfluidic devices air bubbles droplets blood flow Science & Technology |
title_short |
Deformation of red blood cells, air bubbles, and droplets in microfluidic devices: flow visualizations and measurements |
title_full |
Deformation of red blood cells, air bubbles, and droplets in microfluidic devices: flow visualizations and measurements |
title_fullStr |
Deformation of red blood cells, air bubbles, and droplets in microfluidic devices: flow visualizations and measurements |
title_full_unstemmed |
Deformation of red blood cells, air bubbles, and droplets in microfluidic devices: flow visualizations and measurements |
title_sort |
Deformation of red blood cells, air bubbles, and droplets in microfluidic devices: flow visualizations and measurements |
author |
Bento, David |
author_facet |
Bento, David Rodrigues, Raquel O. Faustino, Vera Pinho, Diana Fernandes, Carla S. Pereira, Ana I. Garcia, Valdemar Miranda, João M. Lima, Rui Alberto Madeira Macedo |
author_role |
author |
author2 |
Rodrigues, Raquel O. Faustino, Vera Pinho, Diana Fernandes, Carla S. Pereira, Ana I. Garcia, Valdemar Miranda, João M. Lima, Rui Alberto Madeira Macedo |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Bento, David Rodrigues, Raquel O. Faustino, Vera Pinho, Diana Fernandes, Carla S. Pereira, Ana I. Garcia, Valdemar Miranda, João M. Lima, Rui Alberto Madeira Macedo |
dc.subject.por.fl_str_mv |
red blood cells deformation index microfluidic devices air bubbles droplets blood flow Science & Technology |
topic |
red blood cells deformation index microfluidic devices air bubbles droplets blood flow Science & Technology |
description |
Techniques, such as micropipette aspiration and optical tweezers, are widely used to measure cell mechanical properties, but are generally labor-intensive and time-consuming, typically involving a difficult process of manipulation. In the past two decades, a large number of microfluidic devices have been developed due to the advantages they offer over other techniques, including transparency for direct optical access, lower cost, reduced space and labor, precise control, and easy manipulation of a small volume of blood samples. This review presents recent advances in the development of microfluidic devices to evaluate the mechanical response of individual red blood cells (RBCs) and microbubbles flowing in constriction microchannels. Visualizations and measurements of the deformation of RBCs flowing through hyperbolic, smooth, and sudden-contraction microchannels were evaluated and compared. In particular, we show the potential of using hyperbolic-shaped microchannels to precisely control and assess small changes in RBC deformability in both physiological and pathological situations. Moreover, deformations of air microbubbles and droplets flowing through a microfluidic constriction were also compared with RBCs deformability. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-03-27 2018-03-27T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/60126 |
url |
http://hdl.handle.net/1822/60126 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2072-666X 10.3390/mi9040151 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute |
publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132356524638208 |