Biological nitrogen fixation by Phaseolus vulgaris

Detalhes bibliográficos
Autor(a) principal: Soares, Ricardo
Data de Publicação: 2019
Outros Autores: Tejo, Jesus, Veloso, Maria Manuela, Castro, Isabel Videira e
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://doi.org/10.19084/RCA16104
Resumo: Phaseolus vulgaris L. (common bean) is considered a promiscuous legume in its association with rhizobia since it can be nodulated by several species of the Rhizobiaceae family. In a field experiment in Oeiras (Portugal), strains were isolated from the root nodules of P. vulgaris 675. Each strain was inoculated in two P. vulgaris landraces: Pv 648 and Pv 675. Phylogeny of 16S rRNA, recA and nodC genes were inferred, using partial gene sequences. The results of plant inoculation assays have shown that only one of the isolated strains could successfully nodulate at least the P. vulgarislandrace (Pv 675), with an index of effectiveness of 31%. This strain was identified as belonging to Rhizobium sullae species by the 16S rRNA and recA genes but it also has a nodC gene from Rhizobium gallicum. The remaining strains were identified as belonging to R. gallicum, Agrobacterium tumefaciens and A. arsenijevicii.
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spelling Biological nitrogen fixation by Phaseolus vulgarisFixação biológica de azoto em Phaseolus vulgarisGeralPhaseolus vulgaris L. (common bean) is considered a promiscuous legume in its association with rhizobia since it can be nodulated by several species of the Rhizobiaceae family. In a field experiment in Oeiras (Portugal), strains were isolated from the root nodules of P. vulgaris 675. Each strain was inoculated in two P. vulgaris landraces: Pv 648 and Pv 675. Phylogeny of 16S rRNA, recA and nodC genes were inferred, using partial gene sequences. The results of plant inoculation assays have shown that only one of the isolated strains could successfully nodulate at least the P. vulgarislandrace (Pv 675), with an index of effectiveness of 31%. This strain was identified as belonging to Rhizobium sullae species by the 16S rRNA and recA genes but it also has a nodC gene from Rhizobium gallicum. The remaining strains were identified as belonging to R. gallicum, Agrobacterium tumefaciens and A. arsenijevicii.Phaseolus vulgaris L., conhecido por feijão comum, é uma leguminosa promíscua que é nodulada por várias espécies de bactérias da família Rhizobiaceae. No decorrer de um estudo sobre bactérias Rhizobium que nodulam esta leguminosa, foram isoladas estirpes de bactérias dos nódulos radiculares de P. vulgaris (landrace 675), num ensaio de campo instalado em Oeiras, Portugal. Para a caracterização das estirpes recorreu-se à filogenia dos genes 16S rRNA, recA e nodC, usando sequências parciais destes genes. Os resultados dos ensaios efetuados com dois tipos de feijão comum, 675 e 648, revelaram que apenas uma estirpe foi capaz de nodular uma das “landraces” de P. vulgaris testadas, obtendo 31% de eficácia simbiótica com P. vulgaris 675. Esta estirpe foi identificada como Rhizobium sullae, possuindo no entanto o gene nodC relativo à espécie Rhizobium gallicum. As restantes estirpes isoladas foram identificadas como R. gallicum, Agrobacterium tumefaciens e A. arsenijevicii.Sociedade de Ciências Agrárias de Portugal2019-01-07T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://doi.org/10.19084/RCA16104eng2183-041X0871-018XSoares, RicardoTejo, JesusVeloso, Maria ManuelaCastro, Isabel Videira einfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-09-06T09:24:27ZPortal AgregadorONG
dc.title.none.fl_str_mv Biological nitrogen fixation by Phaseolus vulgaris
Fixação biológica de azoto em Phaseolus vulgaris
title Biological nitrogen fixation by Phaseolus vulgaris
spellingShingle Biological nitrogen fixation by Phaseolus vulgaris
Soares, Ricardo
Geral
title_short Biological nitrogen fixation by Phaseolus vulgaris
title_full Biological nitrogen fixation by Phaseolus vulgaris
title_fullStr Biological nitrogen fixation by Phaseolus vulgaris
title_full_unstemmed Biological nitrogen fixation by Phaseolus vulgaris
title_sort Biological nitrogen fixation by Phaseolus vulgaris
author Soares, Ricardo
author_facet Soares, Ricardo
Tejo, Jesus
Veloso, Maria Manuela
Castro, Isabel Videira e
author_role author
author2 Tejo, Jesus
Veloso, Maria Manuela
Castro, Isabel Videira e
author2_role author
author
author
dc.contributor.author.fl_str_mv Soares, Ricardo
Tejo, Jesus
Veloso, Maria Manuela
Castro, Isabel Videira e
dc.subject.por.fl_str_mv Geral
topic Geral
description Phaseolus vulgaris L. (common bean) is considered a promiscuous legume in its association with rhizobia since it can be nodulated by several species of the Rhizobiaceae family. In a field experiment in Oeiras (Portugal), strains were isolated from the root nodules of P. vulgaris 675. Each strain was inoculated in two P. vulgaris landraces: Pv 648 and Pv 675. Phylogeny of 16S rRNA, recA and nodC genes were inferred, using partial gene sequences. The results of plant inoculation assays have shown that only one of the isolated strains could successfully nodulate at least the P. vulgarislandrace (Pv 675), with an index of effectiveness of 31%. This strain was identified as belonging to Rhizobium sullae species by the 16S rRNA and recA genes but it also has a nodC gene from Rhizobium gallicum. The remaining strains were identified as belonging to R. gallicum, Agrobacterium tumefaciens and A. arsenijevicii.
publishDate 2019
dc.date.none.fl_str_mv 2019-01-07T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.19084/RCA16104
url https://doi.org/10.19084/RCA16104
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2183-041X
0871-018X
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Sociedade de Ciências Agrárias de Portugal
publisher.none.fl_str_mv Sociedade de Ciências Agrárias de Portugal
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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instacron_str RCAAP
institution RCAAP
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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