PCR screening of tick-borne agents in sensitive conservation areas, Southeast Portugal
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.18/4456 |
Resumo: | The Southeast region of Portugal, particularly the Guadiana valley, is currently the reintroduction territory of Lynx pardinus (Iberian lynx), one of the most endangered felids in the world that is only found in the Iberian Peninsula. Over the last century, populations have declined, placing L. pardinus at extremely high risk of extinction in the wild and relying on reintroduction projects. Among the aspects taken into account in the establishment of new populations is the sanitary status of the selected habitats, especially concerning infectious diseases, including tick-borne pathogens (TBPs). This study presents the results of TBPs survey on ticks collected at sensitive conservation areas of Southeast Portugal. From 2012 to 2014, 231 ticks obtained from vegetation, sympatric domestic and wild animals were submitted for analysis. The presence of Babesia spp., Cytauxzoon spp., Theileria spp., Hepatozoon spp., Anaplasma spp., Ehrlichia spp., Candidatus Neoehrlichia mikurensis, among other Anaplasmataceae, and Coxiella burnetii were investigated by PCR. Six tick species were recorded, Dermacentor marginatus (n = 13/5.6%), Hyalomma lusitanicum (n = 175/75.8%), Ixodes ricinus (n = 4/1.7%), Rhipicephalus bursa (n = 7/3.0%), R. pusillus (n = 21/9.1%) and R. sanguineus sensu lato (n = 11/4.8%). The molecular screening confirmed the presence of two tick-borne pathogens, C. burnetii (N = 34) and Anaplasma platys (N = 1), and one tick-endosymbiont, Candidatus Midichloria mitochondrii (N = 45). The results obtained provide new information on the circulation of ticks and TBPs with potential veterinary importance in Iberian lynx habitat. |
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PCR screening of tick-borne agents in sensitive conservation areas, Southeast PortugalAnaplasma PlatysCandidatus Midichloria MitochondriiCoxiella BurnetiiHyalomma LusitanicumIberian Lynx HabitatTick-borne AgentsTicksSoutheast PortugalInfecções Sistémicas e ZoonosesThe Southeast region of Portugal, particularly the Guadiana valley, is currently the reintroduction territory of Lynx pardinus (Iberian lynx), one of the most endangered felids in the world that is only found in the Iberian Peninsula. Over the last century, populations have declined, placing L. pardinus at extremely high risk of extinction in the wild and relying on reintroduction projects. Among the aspects taken into account in the establishment of new populations is the sanitary status of the selected habitats, especially concerning infectious diseases, including tick-borne pathogens (TBPs). This study presents the results of TBPs survey on ticks collected at sensitive conservation areas of Southeast Portugal. From 2012 to 2014, 231 ticks obtained from vegetation, sympatric domestic and wild animals were submitted for analysis. The presence of Babesia spp., Cytauxzoon spp., Theileria spp., Hepatozoon spp., Anaplasma spp., Ehrlichia spp., Candidatus Neoehrlichia mikurensis, among other Anaplasmataceae, and Coxiella burnetii were investigated by PCR. Six tick species were recorded, Dermacentor marginatus (n = 13/5.6%), Hyalomma lusitanicum (n = 175/75.8%), Ixodes ricinus (n = 4/1.7%), Rhipicephalus bursa (n = 7/3.0%), R. pusillus (n = 21/9.1%) and R. sanguineus sensu lato (n = 11/4.8%). The molecular screening confirmed the presence of two tick-borne pathogens, C. burnetii (N = 34) and Anaplasma platys (N = 1), and one tick-endosymbiont, Candidatus Midichloria mitochondrii (N = 45). The results obtained provide new information on the circulation of ticks and TBPs with potential veterinary importance in Iberian lynx habitat.This research was supported by Programa LIFE-Natureza da Comissão Europeia [project reference LIFE08NATP000227] and the Fundação para a Ciencia e Tecnologia [projects references PTDC/SAU-SAP/115266/2009, PTDC/CVT-EPI/4339/2012 and GHTMeUID/Multi/04413/2013]. Hepatozoon canis DNA control was kindly provided by Carla Maia DVM PhD, Instituto de Higiene e Medicina Tropical (IHMT).ElsevierRepositório Científico do Instituto Nacional de SaúdeSantos-Silva, M.M.Melo, P.Santos, N.Antunes, S.Duarte, L.R.Ferrolho, J.Milhano, N.Santos, P.T.Domingos, A.Santos, A.S.2021-11-26T01:30:10Z2016-112016-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/4456engMol Cell Probes. 2017 Feb;31:42-45. doi: 10.1016/j.mcp.2016.11.005. Epub 2016 Nov 250890-850810.1016/j.mcp.2016.11.005info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:40:22Zoai:repositorio.insa.pt:10400.18/4456Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:39:17.147169Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
PCR screening of tick-borne agents in sensitive conservation areas, Southeast Portugal |
title |
PCR screening of tick-borne agents in sensitive conservation areas, Southeast Portugal |
spellingShingle |
PCR screening of tick-borne agents in sensitive conservation areas, Southeast Portugal Santos-Silva, M.M. Anaplasma Platys Candidatus Midichloria Mitochondrii Coxiella Burnetii Hyalomma Lusitanicum Iberian Lynx Habitat Tick-borne Agents Ticks Southeast Portugal Infecções Sistémicas e Zoonoses |
title_short |
PCR screening of tick-borne agents in sensitive conservation areas, Southeast Portugal |
title_full |
PCR screening of tick-borne agents in sensitive conservation areas, Southeast Portugal |
title_fullStr |
PCR screening of tick-borne agents in sensitive conservation areas, Southeast Portugal |
title_full_unstemmed |
PCR screening of tick-borne agents in sensitive conservation areas, Southeast Portugal |
title_sort |
PCR screening of tick-borne agents in sensitive conservation areas, Southeast Portugal |
author |
Santos-Silva, M.M. |
author_facet |
Santos-Silva, M.M. Melo, P. Santos, N. Antunes, S. Duarte, L.R. Ferrolho, J. Milhano, N. Santos, P.T. Domingos, A. Santos, A.S. |
author_role |
author |
author2 |
Melo, P. Santos, N. Antunes, S. Duarte, L.R. Ferrolho, J. Milhano, N. Santos, P.T. Domingos, A. Santos, A.S. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
dc.contributor.author.fl_str_mv |
Santos-Silva, M.M. Melo, P. Santos, N. Antunes, S. Duarte, L.R. Ferrolho, J. Milhano, N. Santos, P.T. Domingos, A. Santos, A.S. |
dc.subject.por.fl_str_mv |
Anaplasma Platys Candidatus Midichloria Mitochondrii Coxiella Burnetii Hyalomma Lusitanicum Iberian Lynx Habitat Tick-borne Agents Ticks Southeast Portugal Infecções Sistémicas e Zoonoses |
topic |
Anaplasma Platys Candidatus Midichloria Mitochondrii Coxiella Burnetii Hyalomma Lusitanicum Iberian Lynx Habitat Tick-borne Agents Ticks Southeast Portugal Infecções Sistémicas e Zoonoses |
description |
The Southeast region of Portugal, particularly the Guadiana valley, is currently the reintroduction territory of Lynx pardinus (Iberian lynx), one of the most endangered felids in the world that is only found in the Iberian Peninsula. Over the last century, populations have declined, placing L. pardinus at extremely high risk of extinction in the wild and relying on reintroduction projects. Among the aspects taken into account in the establishment of new populations is the sanitary status of the selected habitats, especially concerning infectious diseases, including tick-borne pathogens (TBPs). This study presents the results of TBPs survey on ticks collected at sensitive conservation areas of Southeast Portugal. From 2012 to 2014, 231 ticks obtained from vegetation, sympatric domestic and wild animals were submitted for analysis. The presence of Babesia spp., Cytauxzoon spp., Theileria spp., Hepatozoon spp., Anaplasma spp., Ehrlichia spp., Candidatus Neoehrlichia mikurensis, among other Anaplasmataceae, and Coxiella burnetii were investigated by PCR. Six tick species were recorded, Dermacentor marginatus (n = 13/5.6%), Hyalomma lusitanicum (n = 175/75.8%), Ixodes ricinus (n = 4/1.7%), Rhipicephalus bursa (n = 7/3.0%), R. pusillus (n = 21/9.1%) and R. sanguineus sensu lato (n = 11/4.8%). The molecular screening confirmed the presence of two tick-borne pathogens, C. burnetii (N = 34) and Anaplasma platys (N = 1), and one tick-endosymbiont, Candidatus Midichloria mitochondrii (N = 45). The results obtained provide new information on the circulation of ticks and TBPs with potential veterinary importance in Iberian lynx habitat. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-11 2016-11-01T00:00:00Z 2021-11-26T01:30:10Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/4456 |
url |
http://hdl.handle.net/10400.18/4456 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Mol Cell Probes. 2017 Feb;31:42-45. doi: 10.1016/j.mcp.2016.11.005. Epub 2016 Nov 25 0890-8508 10.1016/j.mcp.2016.11.005 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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