In Campylobacter jejuni, a new type of chaperone receives heme from ferrochelatase

Detalhes bibliográficos
Autor(a) principal: Zamarreño Beas, Jordi
Data de Publicação: 2023
Outros Autores: Videira, Marco A.M., Karavaeva, Val, Lourenço, Frederico M., Almeida, Mafalda R., Sousa, Filipa, Saraiva, Lígia M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/158523
Resumo: Funding Information: JZ is a recipient of the MSCA-IF-2019 Individual Fellowship H2020-WF-02-2019, 101003441. FS acknowledges support from the European Research Council (ERC) under the European Union’s Horizon 2020 Research and Innovation program (grant agreement 803768). This work was also financially supported by Fundação para a Ciência e Tecnologia (Portugal) through PTDC/BIA-BQM/28642/2017 grant (LS), the MOSTMICRO-ITQB R&D Unit (UIDB/04612/2020 and UIDP/04612/2020), and the LS4FUTURE Associated Laboratory (LA/P/0087/2020). Publisher Copyright: Copyright © 2023 Zamarreño Beas, Videira, Karavaeva, Lourenço, Almeida, Sousa and Saraiva.
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spelling In Campylobacter jejuni, a new type of chaperone receives heme from ferrochelatasebiogenesisCampylobacterchaperoneferrochelatasehemeMolecular MedicineGeneticsGenetics(clinical)Funding Information: JZ is a recipient of the MSCA-IF-2019 Individual Fellowship H2020-WF-02-2019, 101003441. FS acknowledges support from the European Research Council (ERC) under the European Union’s Horizon 2020 Research and Innovation program (grant agreement 803768). This work was also financially supported by Fundação para a Ciência e Tecnologia (Portugal) through PTDC/BIA-BQM/28642/2017 grant (LS), the MOSTMICRO-ITQB R&D Unit (UIDB/04612/2020 and UIDP/04612/2020), and the LS4FUTURE Associated Laboratory (LA/P/0087/2020). Publisher Copyright: Copyright © 2023 Zamarreño Beas, Videira, Karavaeva, Lourenço, Almeida, Sousa and Saraiva.Intracellular heme formation and trafficking are fundamental processes in living organisms. Bacteria and archaea utilize three biogenesis pathways to produce iron protoporphyrin IX (heme b) that diverge after the formation of the common intermediate uroporphyrinogen III (uro’gen III). In this study, we identify and provide a detailed characterization of the enzymes involved in the transformation of uro’gen III into heme in Campylobacter jejuni, demonstrating that this bacterium utilizes the protoporphyrin-dependent (PPD) pathway. In general, limited knowledge exists regarding the mechanisms by which heme b reaches its target proteins after this final step. Specifically, the chaperones necessary for trafficking heme to prevent the cytotoxic effects associated with free heme remain largely unidentified. In C. jejuni, we identified a protein named CgdH2 that binds heme with a dissociation constant of 4.9 ± 1.0 µM, and this binding is impaired upon mutation of residues histidine 45 and 133. We demonstrate that C. jejuni CgdH2 establishes protein–protein interactions with ferrochelatase, suggesting its role in facilitating heme transfer from ferrochelatase to CgdH2. Furthermore, phylogenetic analysis reveals that C. jejuni CgdH2 is evolutionarily distinct from the currently known chaperones. Therefore, CgdH2 is the first protein identified as an acceptor of intracellularly formed heme, expanding our knowledge of the mechanisms underlying heme trafficking within bacterial cells.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNZamarreño Beas, JordiVideira, Marco A.M.Karavaeva, ValLourenço, Frederico M.Almeida, Mafalda R.Sousa, FilipaSaraiva, Lígia M.2023-09-30T22:18:48Z20232023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/158523eng1664-8021PURE: 72367129https://doi.org/10.3389/fgene.2023.1199357info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:41:00Zoai:run.unl.pt:10362/158523Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:57:11.195159Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv In Campylobacter jejuni, a new type of chaperone receives heme from ferrochelatase
title In Campylobacter jejuni, a new type of chaperone receives heme from ferrochelatase
spellingShingle In Campylobacter jejuni, a new type of chaperone receives heme from ferrochelatase
Zamarreño Beas, Jordi
biogenesis
Campylobacter
chaperone
ferrochelatase
heme
Molecular Medicine
Genetics
Genetics(clinical)
title_short In Campylobacter jejuni, a new type of chaperone receives heme from ferrochelatase
title_full In Campylobacter jejuni, a new type of chaperone receives heme from ferrochelatase
title_fullStr In Campylobacter jejuni, a new type of chaperone receives heme from ferrochelatase
title_full_unstemmed In Campylobacter jejuni, a new type of chaperone receives heme from ferrochelatase
title_sort In Campylobacter jejuni, a new type of chaperone receives heme from ferrochelatase
author Zamarreño Beas, Jordi
author_facet Zamarreño Beas, Jordi
Videira, Marco A.M.
Karavaeva, Val
Lourenço, Frederico M.
Almeida, Mafalda R.
Sousa, Filipa
Saraiva, Lígia M.
author_role author
author2 Videira, Marco A.M.
Karavaeva, Val
Lourenço, Frederico M.
Almeida, Mafalda R.
Sousa, Filipa
Saraiva, Lígia M.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
RUN
dc.contributor.author.fl_str_mv Zamarreño Beas, Jordi
Videira, Marco A.M.
Karavaeva, Val
Lourenço, Frederico M.
Almeida, Mafalda R.
Sousa, Filipa
Saraiva, Lígia M.
dc.subject.por.fl_str_mv biogenesis
Campylobacter
chaperone
ferrochelatase
heme
Molecular Medicine
Genetics
Genetics(clinical)
topic biogenesis
Campylobacter
chaperone
ferrochelatase
heme
Molecular Medicine
Genetics
Genetics(clinical)
description Funding Information: JZ is a recipient of the MSCA-IF-2019 Individual Fellowship H2020-WF-02-2019, 101003441. FS acknowledges support from the European Research Council (ERC) under the European Union’s Horizon 2020 Research and Innovation program (grant agreement 803768). This work was also financially supported by Fundação para a Ciência e Tecnologia (Portugal) through PTDC/BIA-BQM/28642/2017 grant (LS), the MOSTMICRO-ITQB R&D Unit (UIDB/04612/2020 and UIDP/04612/2020), and the LS4FUTURE Associated Laboratory (LA/P/0087/2020). Publisher Copyright: Copyright © 2023 Zamarreño Beas, Videira, Karavaeva, Lourenço, Almeida, Sousa and Saraiva.
publishDate 2023
dc.date.none.fl_str_mv 2023-09-30T22:18:48Z
2023
2023-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/158523
url http://hdl.handle.net/10362/158523
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1664-8021
PURE: 72367129
https://doi.org/10.3389/fgene.2023.1199357
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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