Glyphosate-dependent effects on photosynthesis of Solanum lycopersicum L.-An ecophysiological, ultrastructural and molecular approach

Detalhes bibliográficos
Autor(a) principal: Soares, Cristiano
Data de Publicação: 2020
Outros Autores: Pereira, Ruth, Martins, Maria, Tamagnini, Paula, Serôdio, João, Moutinho-Pereira, José, Cunha, Ana, Fidalgo, Fernanda
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10773/37241
Resumo: This study aimed to assess the toxicity of glyphosate (GLY; 0, 10, 20 and 30 mg kg-1) in Solanum lycopersicum L., particularly focusing on the photosynthetic metabolism. By combining ecophysiological, ultrastructural, biochemical and molecular tools, the results revealed that the exposure of tomato plants to GLY led to alterations in leaf water balance regulation [increasing stomatal conductance (gs) and decreasing water use efficiency (WUEi) at higher concentrations] and induced slight alterations in the structural integrity of cells, mainly in chloroplasts, accompanied by a loss of cell viability. Moreover, the transcriptional and biochemical control of several photosynthetic-related parameters was reduced upon GLY exposure. However, in vivo chlorophyll fluorometry and IRGA gas-exchange studies revealed that the photosynthetic yield of S. lycopersicum was not repressed by GLY. Overall, GLY impacts cellular and subcellular homeostasis (by affecting chloroplast structure, reducing photosynthetic pigments and inhibiting photosynthetic-related genes transcription), and leaf structure, but is not reducing the carbon flow on a leaf area basis. Altogether, these results suggest a trade-off effect in which GLY-induced toxicity is compensated by a higher photosynthetic activity related to GLY-induced dysfunction in gs and an increase in mesophyll thickness/density, allowing the viable leaf cells to maintain their photosynthetic capacity.
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spelling Glyphosate-dependent effects on photosynthesis of Solanum lycopersicum L.-An ecophysiological, ultrastructural and molecular approachNon-target plantsAbiotic stressPhotochemistryCalvin cycleChlorophyll fluorometryGas-exchangeThis study aimed to assess the toxicity of glyphosate (GLY; 0, 10, 20 and 30 mg kg-1) in Solanum lycopersicum L., particularly focusing on the photosynthetic metabolism. By combining ecophysiological, ultrastructural, biochemical and molecular tools, the results revealed that the exposure of tomato plants to GLY led to alterations in leaf water balance regulation [increasing stomatal conductance (gs) and decreasing water use efficiency (WUEi) at higher concentrations] and induced slight alterations in the structural integrity of cells, mainly in chloroplasts, accompanied by a loss of cell viability. Moreover, the transcriptional and biochemical control of several photosynthetic-related parameters was reduced upon GLY exposure. However, in vivo chlorophyll fluorometry and IRGA gas-exchange studies revealed that the photosynthetic yield of S. lycopersicum was not repressed by GLY. Overall, GLY impacts cellular and subcellular homeostasis (by affecting chloroplast structure, reducing photosynthetic pigments and inhibiting photosynthetic-related genes transcription), and leaf structure, but is not reducing the carbon flow on a leaf area basis. Altogether, these results suggest a trade-off effect in which GLY-induced toxicity is compensated by a higher photosynthetic activity related to GLY-induced dysfunction in gs and an increase in mesophyll thickness/density, allowing the viable leaf cells to maintain their photosynthetic capacity.Elsevier2023-04-20T14:40:53Z2020-11-05T00:00:00Z2020-11-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/37241eng0304-389410.1016/j.jhazmat.2020.122871Soares, CristianoPereira, RuthMartins, MariaTamagnini, PaulaSerôdio, JoãoMoutinho-Pereira, JoséCunha, AnaFidalgo, Fernandainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T12:11:49Zoai:ria.ua.pt:10773/37241Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:07:51.483429Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Glyphosate-dependent effects on photosynthesis of Solanum lycopersicum L.-An ecophysiological, ultrastructural and molecular approach
title Glyphosate-dependent effects on photosynthesis of Solanum lycopersicum L.-An ecophysiological, ultrastructural and molecular approach
spellingShingle Glyphosate-dependent effects on photosynthesis of Solanum lycopersicum L.-An ecophysiological, ultrastructural and molecular approach
Soares, Cristiano
Non-target plants
Abiotic stress
Photochemistry
Calvin cycle
Chlorophyll fluorometry
Gas-exchange
title_short Glyphosate-dependent effects on photosynthesis of Solanum lycopersicum L.-An ecophysiological, ultrastructural and molecular approach
title_full Glyphosate-dependent effects on photosynthesis of Solanum lycopersicum L.-An ecophysiological, ultrastructural and molecular approach
title_fullStr Glyphosate-dependent effects on photosynthesis of Solanum lycopersicum L.-An ecophysiological, ultrastructural and molecular approach
title_full_unstemmed Glyphosate-dependent effects on photosynthesis of Solanum lycopersicum L.-An ecophysiological, ultrastructural and molecular approach
title_sort Glyphosate-dependent effects on photosynthesis of Solanum lycopersicum L.-An ecophysiological, ultrastructural and molecular approach
author Soares, Cristiano
author_facet Soares, Cristiano
Pereira, Ruth
Martins, Maria
Tamagnini, Paula
Serôdio, João
Moutinho-Pereira, José
Cunha, Ana
Fidalgo, Fernanda
author_role author
author2 Pereira, Ruth
Martins, Maria
Tamagnini, Paula
Serôdio, João
Moutinho-Pereira, José
Cunha, Ana
Fidalgo, Fernanda
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Soares, Cristiano
Pereira, Ruth
Martins, Maria
Tamagnini, Paula
Serôdio, João
Moutinho-Pereira, José
Cunha, Ana
Fidalgo, Fernanda
dc.subject.por.fl_str_mv Non-target plants
Abiotic stress
Photochemistry
Calvin cycle
Chlorophyll fluorometry
Gas-exchange
topic Non-target plants
Abiotic stress
Photochemistry
Calvin cycle
Chlorophyll fluorometry
Gas-exchange
description This study aimed to assess the toxicity of glyphosate (GLY; 0, 10, 20 and 30 mg kg-1) in Solanum lycopersicum L., particularly focusing on the photosynthetic metabolism. By combining ecophysiological, ultrastructural, biochemical and molecular tools, the results revealed that the exposure of tomato plants to GLY led to alterations in leaf water balance regulation [increasing stomatal conductance (gs) and decreasing water use efficiency (WUEi) at higher concentrations] and induced slight alterations in the structural integrity of cells, mainly in chloroplasts, accompanied by a loss of cell viability. Moreover, the transcriptional and biochemical control of several photosynthetic-related parameters was reduced upon GLY exposure. However, in vivo chlorophyll fluorometry and IRGA gas-exchange studies revealed that the photosynthetic yield of S. lycopersicum was not repressed by GLY. Overall, GLY impacts cellular and subcellular homeostasis (by affecting chloroplast structure, reducing photosynthetic pigments and inhibiting photosynthetic-related genes transcription), and leaf structure, but is not reducing the carbon flow on a leaf area basis. Altogether, these results suggest a trade-off effect in which GLY-induced toxicity is compensated by a higher photosynthetic activity related to GLY-induced dysfunction in gs and an increase in mesophyll thickness/density, allowing the viable leaf cells to maintain their photosynthetic capacity.
publishDate 2020
dc.date.none.fl_str_mv 2020-11-05T00:00:00Z
2020-11-05
2023-04-20T14:40:53Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10773/37241
url http://hdl.handle.net/10773/37241
dc.language.iso.fl_str_mv eng
language eng
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10.1016/j.jhazmat.2020.122871
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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