Glyphosate-dependent effects on photosynthesis of Solanum lycopersicum L.-An ecophysiological, ultrastructural and molecular approach
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/37241 |
Resumo: | This study aimed to assess the toxicity of glyphosate (GLY; 0, 10, 20 and 30 mg kg-1) in Solanum lycopersicum L., particularly focusing on the photosynthetic metabolism. By combining ecophysiological, ultrastructural, biochemical and molecular tools, the results revealed that the exposure of tomato plants to GLY led to alterations in leaf water balance regulation [increasing stomatal conductance (gs) and decreasing water use efficiency (WUEi) at higher concentrations] and induced slight alterations in the structural integrity of cells, mainly in chloroplasts, accompanied by a loss of cell viability. Moreover, the transcriptional and biochemical control of several photosynthetic-related parameters was reduced upon GLY exposure. However, in vivo chlorophyll fluorometry and IRGA gas-exchange studies revealed that the photosynthetic yield of S. lycopersicum was not repressed by GLY. Overall, GLY impacts cellular and subcellular homeostasis (by affecting chloroplast structure, reducing photosynthetic pigments and inhibiting photosynthetic-related genes transcription), and leaf structure, but is not reducing the carbon flow on a leaf area basis. Altogether, these results suggest a trade-off effect in which GLY-induced toxicity is compensated by a higher photosynthetic activity related to GLY-induced dysfunction in gs and an increase in mesophyll thickness/density, allowing the viable leaf cells to maintain their photosynthetic capacity. |
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Glyphosate-dependent effects on photosynthesis of Solanum lycopersicum L.-An ecophysiological, ultrastructural and molecular approachNon-target plantsAbiotic stressPhotochemistryCalvin cycleChlorophyll fluorometryGas-exchangeThis study aimed to assess the toxicity of glyphosate (GLY; 0, 10, 20 and 30 mg kg-1) in Solanum lycopersicum L., particularly focusing on the photosynthetic metabolism. By combining ecophysiological, ultrastructural, biochemical and molecular tools, the results revealed that the exposure of tomato plants to GLY led to alterations in leaf water balance regulation [increasing stomatal conductance (gs) and decreasing water use efficiency (WUEi) at higher concentrations] and induced slight alterations in the structural integrity of cells, mainly in chloroplasts, accompanied by a loss of cell viability. Moreover, the transcriptional and biochemical control of several photosynthetic-related parameters was reduced upon GLY exposure. However, in vivo chlorophyll fluorometry and IRGA gas-exchange studies revealed that the photosynthetic yield of S. lycopersicum was not repressed by GLY. Overall, GLY impacts cellular and subcellular homeostasis (by affecting chloroplast structure, reducing photosynthetic pigments and inhibiting photosynthetic-related genes transcription), and leaf structure, but is not reducing the carbon flow on a leaf area basis. Altogether, these results suggest a trade-off effect in which GLY-induced toxicity is compensated by a higher photosynthetic activity related to GLY-induced dysfunction in gs and an increase in mesophyll thickness/density, allowing the viable leaf cells to maintain their photosynthetic capacity.Elsevier2023-04-20T14:40:53Z2020-11-05T00:00:00Z2020-11-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/37241eng0304-389410.1016/j.jhazmat.2020.122871Soares, CristianoPereira, RuthMartins, MariaTamagnini, PaulaSerôdio, JoãoMoutinho-Pereira, JoséCunha, AnaFidalgo, Fernandainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T12:11:49Zoai:ria.ua.pt:10773/37241Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:07:51.483429Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Glyphosate-dependent effects on photosynthesis of Solanum lycopersicum L.-An ecophysiological, ultrastructural and molecular approach |
title |
Glyphosate-dependent effects on photosynthesis of Solanum lycopersicum L.-An ecophysiological, ultrastructural and molecular approach |
spellingShingle |
Glyphosate-dependent effects on photosynthesis of Solanum lycopersicum L.-An ecophysiological, ultrastructural and molecular approach Soares, Cristiano Non-target plants Abiotic stress Photochemistry Calvin cycle Chlorophyll fluorometry Gas-exchange |
title_short |
Glyphosate-dependent effects on photosynthesis of Solanum lycopersicum L.-An ecophysiological, ultrastructural and molecular approach |
title_full |
Glyphosate-dependent effects on photosynthesis of Solanum lycopersicum L.-An ecophysiological, ultrastructural and molecular approach |
title_fullStr |
Glyphosate-dependent effects on photosynthesis of Solanum lycopersicum L.-An ecophysiological, ultrastructural and molecular approach |
title_full_unstemmed |
Glyphosate-dependent effects on photosynthesis of Solanum lycopersicum L.-An ecophysiological, ultrastructural and molecular approach |
title_sort |
Glyphosate-dependent effects on photosynthesis of Solanum lycopersicum L.-An ecophysiological, ultrastructural and molecular approach |
author |
Soares, Cristiano |
author_facet |
Soares, Cristiano Pereira, Ruth Martins, Maria Tamagnini, Paula Serôdio, João Moutinho-Pereira, José Cunha, Ana Fidalgo, Fernanda |
author_role |
author |
author2 |
Pereira, Ruth Martins, Maria Tamagnini, Paula Serôdio, João Moutinho-Pereira, José Cunha, Ana Fidalgo, Fernanda |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Soares, Cristiano Pereira, Ruth Martins, Maria Tamagnini, Paula Serôdio, João Moutinho-Pereira, José Cunha, Ana Fidalgo, Fernanda |
dc.subject.por.fl_str_mv |
Non-target plants Abiotic stress Photochemistry Calvin cycle Chlorophyll fluorometry Gas-exchange |
topic |
Non-target plants Abiotic stress Photochemistry Calvin cycle Chlorophyll fluorometry Gas-exchange |
description |
This study aimed to assess the toxicity of glyphosate (GLY; 0, 10, 20 and 30 mg kg-1) in Solanum lycopersicum L., particularly focusing on the photosynthetic metabolism. By combining ecophysiological, ultrastructural, biochemical and molecular tools, the results revealed that the exposure of tomato plants to GLY led to alterations in leaf water balance regulation [increasing stomatal conductance (gs) and decreasing water use efficiency (WUEi) at higher concentrations] and induced slight alterations in the structural integrity of cells, mainly in chloroplasts, accompanied by a loss of cell viability. Moreover, the transcriptional and biochemical control of several photosynthetic-related parameters was reduced upon GLY exposure. However, in vivo chlorophyll fluorometry and IRGA gas-exchange studies revealed that the photosynthetic yield of S. lycopersicum was not repressed by GLY. Overall, GLY impacts cellular and subcellular homeostasis (by affecting chloroplast structure, reducing photosynthetic pigments and inhibiting photosynthetic-related genes transcription), and leaf structure, but is not reducing the carbon flow on a leaf area basis. Altogether, these results suggest a trade-off effect in which GLY-induced toxicity is compensated by a higher photosynthetic activity related to GLY-induced dysfunction in gs and an increase in mesophyll thickness/density, allowing the viable leaf cells to maintain their photosynthetic capacity. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-11-05T00:00:00Z 2020-11-05 2023-04-20T14:40:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/37241 |
url |
http://hdl.handle.net/10773/37241 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0304-3894 10.1016/j.jhazmat.2020.122871 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799137732521361408 |