Annona coriacea Mart. fractions promote cell cycle arrest and inhibit autophagic flux in human cervical cancer cell lines
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/67251 |
Resumo: | Plant-based compounds are an option to explore and perhaps overcome the limitations of current antitumor treatments. Annona coriacea Mart. is a plant with a broad spectrum of biological activities, but its antitumor activity is still unclear. The purpose of our study was to determine the effects of A. coriacea fractions on a panel of cervical cancer cell lines and a normal keratinocyte cell line. The antitumor effect was investigated in vitro by viability assays, cell cycle, apoptosis, migration, and invasion assays. Intracellular signaling was assessed by Western blot, and major compounds were identified by mass spectrometry. All fractions exhibited a cytotoxic effect on cisplatin-resistant cell lines, SiHa and HeLa. C3 and C5 were significantly more cytotoxic and selective than cisplatin in SiHa and Hela cells. However, in CaSki, a cisplatin-sensitive cell line, the compounds did not demonstrate higher cytotoxicity when compared with cisplatin. Alkaloids and acetogenins were the main compounds identified in the fractions. These fractions also markedly decreased cell proliferation with p21 increase and cell cycle arrest in G2/M. These effects were accompanied by an increase of H2AX phosphorylation levels and DNA damage index. In addition, fractions C3 and C5 promoted p62 accumulation and decrease of LC3II, as well as acid vesicle levels, indicating the inhibition of autophagic flow. These findings suggest that A. coriacea fractions may become effective antineoplastic drugs and highlight the autophagy inhibition properties of these fractions in sensitizing cervical cancer cells to treatment. |
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Annona coriacea Mart. fractions promote cell cycle arrest and inhibit autophagic flux in human cervical cancer cell linesAnnonaApoptosisAutophagyCell Cycle CheckpointsCell ProliferationCisplatinDrug Resistance, NeoplasmFemaleHeLa CellsHumansPlant ExtractsSignal TransductionUterine Cervical NeoplasmsNatural compoundsCervical cancerCell cycle arrestCiências Médicas::Medicina BásicaScience & TechnologyPlant-based compounds are an option to explore and perhaps overcome the limitations of current antitumor treatments. Annona coriacea Mart. is a plant with a broad spectrum of biological activities, but its antitumor activity is still unclear. The purpose of our study was to determine the effects of A. coriacea fractions on a panel of cervical cancer cell lines and a normal keratinocyte cell line. The antitumor effect was investigated in vitro by viability assays, cell cycle, apoptosis, migration, and invasion assays. Intracellular signaling was assessed by Western blot, and major compounds were identified by mass spectrometry. All fractions exhibited a cytotoxic effect on cisplatin-resistant cell lines, SiHa and HeLa. C3 and C5 were significantly more cytotoxic and selective than cisplatin in SiHa and Hela cells. However, in CaSki, a cisplatin-sensitive cell line, the compounds did not demonstrate higher cytotoxicity when compared with cisplatin. Alkaloids and acetogenins were the main compounds identified in the fractions. These fractions also markedly decreased cell proliferation with p21 increase and cell cycle arrest in G2/M. These effects were accompanied by an increase of H2AX phosphorylation levels and DNA damage index. In addition, fractions C3 and C5 promoted p62 accumulation and decrease of LC3II, as well as acid vesicle levels, indicating the inhibition of autophagic flow. These findings suggest that A. coriacea fractions may become effective antineoplastic drugs and highlight the autophagy inhibition properties of these fractions in sensitizing cervical cancer cells to treatment.e FINEP (MCTI/FINEP/MS/SCTIE/DECIT-01/ 2013—FP XII-BIOPLAT), Barretos Cancer Hospital, CAPES, CNPq, FAPEMIG, UFSJ. RMR is a recipient of CNPq Productivity GrantMDPIUniversidade do MinhoGomes, Izabela N. FariaSilva-Oliveira, Renato J.Oliveira Silva, Viviane A.Rosa, Marcela N.Vital, Patrik S.Barbosa, Maria Cristina S.Dos Santos, Fábio VieiraJunqueira, João Gabriel M.Severino, Vanessa G. P.Oliveira, Bruno G.Romão, WandersonReis, R. M.Ribeiro, Rosy Iara Maciel de Azambuja2019-11-012019-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/67251engGomes, I. N. F., Silva-Oliveira, R. J., Oliveira Silva, V. A., Rosa, M. N., et. al.(2019). Annona coriacea Mart. Fractions Promote Cell Cycle Arrest and Inhibit Autophagic Flux in Human Cervical Cancer Cell Lines. Molecules, 24(21)1420-304910.3390/molecules2421396331683835https://www.mdpi.com/1420-3049/24/21/3963info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:43:46Zoai:repositorium.sdum.uminho.pt:1822/67251Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:41:19.341905Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Annona coriacea Mart. fractions promote cell cycle arrest and inhibit autophagic flux in human cervical cancer cell lines |
title |
Annona coriacea Mart. fractions promote cell cycle arrest and inhibit autophagic flux in human cervical cancer cell lines |
spellingShingle |
Annona coriacea Mart. fractions promote cell cycle arrest and inhibit autophagic flux in human cervical cancer cell lines Gomes, Izabela N. Faria Annona Apoptosis Autophagy Cell Cycle Checkpoints Cell Proliferation Cisplatin Drug Resistance, Neoplasm Female HeLa Cells Humans Plant Extracts Signal Transduction Uterine Cervical Neoplasms Natural compounds Cervical cancer Cell cycle arrest Ciências Médicas::Medicina Básica Science & Technology |
title_short |
Annona coriacea Mart. fractions promote cell cycle arrest and inhibit autophagic flux in human cervical cancer cell lines |
title_full |
Annona coriacea Mart. fractions promote cell cycle arrest and inhibit autophagic flux in human cervical cancer cell lines |
title_fullStr |
Annona coriacea Mart. fractions promote cell cycle arrest and inhibit autophagic flux in human cervical cancer cell lines |
title_full_unstemmed |
Annona coriacea Mart. fractions promote cell cycle arrest and inhibit autophagic flux in human cervical cancer cell lines |
title_sort |
Annona coriacea Mart. fractions promote cell cycle arrest and inhibit autophagic flux in human cervical cancer cell lines |
author |
Gomes, Izabela N. Faria |
author_facet |
Gomes, Izabela N. Faria Silva-Oliveira, Renato J. Oliveira Silva, Viviane A. Rosa, Marcela N. Vital, Patrik S. Barbosa, Maria Cristina S. Dos Santos, Fábio Vieira Junqueira, João Gabriel M. Severino, Vanessa G. P. Oliveira, Bruno G. Romão, Wanderson Reis, R. M. Ribeiro, Rosy Iara Maciel de Azambuja |
author_role |
author |
author2 |
Silva-Oliveira, Renato J. Oliveira Silva, Viviane A. Rosa, Marcela N. Vital, Patrik S. Barbosa, Maria Cristina S. Dos Santos, Fábio Vieira Junqueira, João Gabriel M. Severino, Vanessa G. P. Oliveira, Bruno G. Romão, Wanderson Reis, R. M. Ribeiro, Rosy Iara Maciel de Azambuja |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Gomes, Izabela N. Faria Silva-Oliveira, Renato J. Oliveira Silva, Viviane A. Rosa, Marcela N. Vital, Patrik S. Barbosa, Maria Cristina S. Dos Santos, Fábio Vieira Junqueira, João Gabriel M. Severino, Vanessa G. P. Oliveira, Bruno G. Romão, Wanderson Reis, R. M. Ribeiro, Rosy Iara Maciel de Azambuja |
dc.subject.por.fl_str_mv |
Annona Apoptosis Autophagy Cell Cycle Checkpoints Cell Proliferation Cisplatin Drug Resistance, Neoplasm Female HeLa Cells Humans Plant Extracts Signal Transduction Uterine Cervical Neoplasms Natural compounds Cervical cancer Cell cycle arrest Ciências Médicas::Medicina Básica Science & Technology |
topic |
Annona Apoptosis Autophagy Cell Cycle Checkpoints Cell Proliferation Cisplatin Drug Resistance, Neoplasm Female HeLa Cells Humans Plant Extracts Signal Transduction Uterine Cervical Neoplasms Natural compounds Cervical cancer Cell cycle arrest Ciências Médicas::Medicina Básica Science & Technology |
description |
Plant-based compounds are an option to explore and perhaps overcome the limitations of current antitumor treatments. Annona coriacea Mart. is a plant with a broad spectrum of biological activities, but its antitumor activity is still unclear. The purpose of our study was to determine the effects of A. coriacea fractions on a panel of cervical cancer cell lines and a normal keratinocyte cell line. The antitumor effect was investigated in vitro by viability assays, cell cycle, apoptosis, migration, and invasion assays. Intracellular signaling was assessed by Western blot, and major compounds were identified by mass spectrometry. All fractions exhibited a cytotoxic effect on cisplatin-resistant cell lines, SiHa and HeLa. C3 and C5 were significantly more cytotoxic and selective than cisplatin in SiHa and Hela cells. However, in CaSki, a cisplatin-sensitive cell line, the compounds did not demonstrate higher cytotoxicity when compared with cisplatin. Alkaloids and acetogenins were the main compounds identified in the fractions. These fractions also markedly decreased cell proliferation with p21 increase and cell cycle arrest in G2/M. These effects were accompanied by an increase of H2AX phosphorylation levels and DNA damage index. In addition, fractions C3 and C5 promoted p62 accumulation and decrease of LC3II, as well as acid vesicle levels, indicating the inhibition of autophagic flow. These findings suggest that A. coriacea fractions may become effective antineoplastic drugs and highlight the autophagy inhibition properties of these fractions in sensitizing cervical cancer cells to treatment. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-11-01 2019-11-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/67251 |
url |
http://hdl.handle.net/1822/67251 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Gomes, I. N. F., Silva-Oliveira, R. J., Oliveira Silva, V. A., Rosa, M. N., et. al.(2019). Annona coriacea Mart. Fractions Promote Cell Cycle Arrest and Inhibit Autophagic Flux in Human Cervical Cancer Cell Lines. Molecules, 24(21) 1420-3049 10.3390/molecules24213963 31683835 https://www.mdpi.com/1420-3049/24/21/3963 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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