Antagonist G-targeted liposomes for improved delivery of anticancer drugs in small cell lung carcinoma

Detalhes bibliográficos
Autor(a) principal: Carvalheiro, Manuela
Data de Publicação: 2022
Outros Autores: Ferreira-Silva, Margarida, Holovanchuk, Denys, Marinho, H. Susana, Moreira, João Nuno, Soares, Helena, Corvo, M. Luisa, Cruz, Maria Eugénia M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.21/14382
Resumo: FCT_UID/Multi/00612/2019. FCT_UIDB/00100/2020. FCT_UIDP/00100/2020.
id RCAP_06f5f4a10d198c7fe6e6e63134522f4d
oai_identifier_str oai:repositorio.ipl.pt:10400.21/14382
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Antagonist G-targeted liposomes for improved delivery of anticancer drugs in small cell lung carcinomaAntineoplastic agentsTherapeutic useDrug delivery systemsLiposomesLung neoplasmsDrug therapyOligopeptidesSmall cell lung carcinomaTissue distributionFCT_UID/Multi/00612/2019FCT_UIDB/00100/2020FCT_UIDP/00100/2020FCT_UID/Multi/00612/2019. FCT_UIDB/00100/2020. FCT_UIDP/00100/2020.Ligand-mediated targeted liposomes have the potential to increase the therapeutic efficacy of anticancer drugs. This work aimed to evaluate the ability of antagonist G, a peptide targeting agent capable of blocking the action of multiple neuropeptides, to selectivity improve targeting and internalization of liposomal formulations (long-circulating liposomes, LCL, and stabilized antisense lipid particles containing ionizable amino lipid, SALP) to H69 and H82 small cell lung carcinoma (SCLC) cell lines. Antagonist G-targeted LCL and SALP were prepared by two different methods (either by direct covalent linkage at activated PEG grafted onto the liposomal surface or by post-insertion of DSPE-PEG-antagonist-G-conjugates into pre-formed liposomes). Association of the liposomal formulations with target SCLC cells was studied by fluorescence microscopy using fluorescence-labeled liposomes and confirmed quantitatively with [3H]-CHE-labelled liposomes. An antisense oligodeoxynucleotide against the overexpressed oncogene c-myc(as(c-myc)) was efficiently loaded into SALP formulations, the encapsulation efficiency decreased due to the inclusion of the targeting ligand. Also, liposome size was affected by as(c-myc) physical-chemical properties. The amount of antagonist G linked to the surface of the liposomal formulations was dependent on the coupling method and lipid composition used. Covalent attachment of antagonist G increased liposomes' cellular association and internalization via receptor-mediated and clathrin-dependent endocytosis, as assessed in SCLC cell lines. Biodistribution studies in healthy mice revealed a preferential lung accumulation of antagonist G-targeted SALP as compared to the non-targeted counterpart. Lung levels of the former were up to 3-fold higher 24 h after administration, highlighting their potential to be used as delivery vectors for SCLC treatment.ElsevierRCIPLCarvalheiro, ManuelaFerreira-Silva, MargaridaHolovanchuk, DenysMarinho, H. SusanaMoreira, João NunoSoares, HelenaCorvo, M. LuisaCruz, Maria Eugénia M.2024-03-01T01:30:21Z2022-012022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.21/14382engCarvalheiro M, Ferreira-Silva M, Holovanchuk D, Marinho S, Moreira JN, Soares H, et al. Antagonist G-targeted liposomes for improved delivery of anticancer drugs in small cell lung carcinoma. Int J Pharmaceutics. 2022;612:121380.10.1016/j.ijpharm.2021.121380info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-06T02:15:56Zoai:repositorio.ipl.pt:10400.21/14382Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:22:11.901657Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Antagonist G-targeted liposomes for improved delivery of anticancer drugs in small cell lung carcinoma
title Antagonist G-targeted liposomes for improved delivery of anticancer drugs in small cell lung carcinoma
spellingShingle Antagonist G-targeted liposomes for improved delivery of anticancer drugs in small cell lung carcinoma
Carvalheiro, Manuela
Antineoplastic agents
Therapeutic use
Drug delivery systems
Liposomes
Lung neoplasms
Drug therapy
Oligopeptides
Small cell lung carcinoma
Tissue distribution
FCT_UID/Multi/00612/2019
FCT_UIDB/00100/2020
FCT_UIDP/00100/2020
title_short Antagonist G-targeted liposomes for improved delivery of anticancer drugs in small cell lung carcinoma
title_full Antagonist G-targeted liposomes for improved delivery of anticancer drugs in small cell lung carcinoma
title_fullStr Antagonist G-targeted liposomes for improved delivery of anticancer drugs in small cell lung carcinoma
title_full_unstemmed Antagonist G-targeted liposomes for improved delivery of anticancer drugs in small cell lung carcinoma
title_sort Antagonist G-targeted liposomes for improved delivery of anticancer drugs in small cell lung carcinoma
author Carvalheiro, Manuela
author_facet Carvalheiro, Manuela
Ferreira-Silva, Margarida
Holovanchuk, Denys
Marinho, H. Susana
Moreira, João Nuno
Soares, Helena
Corvo, M. Luisa
Cruz, Maria Eugénia M.
author_role author
author2 Ferreira-Silva, Margarida
Holovanchuk, Denys
Marinho, H. Susana
Moreira, João Nuno
Soares, Helena
Corvo, M. Luisa
Cruz, Maria Eugénia M.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv RCIPL
dc.contributor.author.fl_str_mv Carvalheiro, Manuela
Ferreira-Silva, Margarida
Holovanchuk, Denys
Marinho, H. Susana
Moreira, João Nuno
Soares, Helena
Corvo, M. Luisa
Cruz, Maria Eugénia M.
dc.subject.por.fl_str_mv Antineoplastic agents
Therapeutic use
Drug delivery systems
Liposomes
Lung neoplasms
Drug therapy
Oligopeptides
Small cell lung carcinoma
Tissue distribution
FCT_UID/Multi/00612/2019
FCT_UIDB/00100/2020
FCT_UIDP/00100/2020
topic Antineoplastic agents
Therapeutic use
Drug delivery systems
Liposomes
Lung neoplasms
Drug therapy
Oligopeptides
Small cell lung carcinoma
Tissue distribution
FCT_UID/Multi/00612/2019
FCT_UIDB/00100/2020
FCT_UIDP/00100/2020
description FCT_UID/Multi/00612/2019. FCT_UIDB/00100/2020. FCT_UIDP/00100/2020.
publishDate 2022
dc.date.none.fl_str_mv 2022-01
2022-01-01T00:00:00Z
2024-03-01T01:30:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.21/14382
url http://hdl.handle.net/10400.21/14382
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Carvalheiro M, Ferreira-Silva M, Holovanchuk D, Marinho S, Moreira JN, Soares H, et al. Antagonist G-targeted liposomes for improved delivery of anticancer drugs in small cell lung carcinoma. Int J Pharmaceutics. 2022;612:121380.
10.1016/j.ijpharm.2021.121380
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799133493817507840

Registros relacionados