ITS rDNA barcodes clarify molecular diversity of aquatic hyphomycetes
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/80639 |
Resumo: | Aquatic hyphomycetes are key microbial decomposers of allochthonous organic matter in freshwater ecosystems. Although their importance in carbon flow and food webs in streams is widely recognized, there are still gaps in our understanding of their molecular diversity and distribution patterns. Our study utilized the growing database of ITS rDNA barcodes of aquatic hyphomycetes (1252 sequences) and aimed to (i) produce new barcodes for some lesser-known taxa; (ii) clarify the taxonomic placement of some taxa at the class or order level, based on molecular data; and (iii) provide insights into the biogeographical origins of some taxa. This study increased the number of aquatic hyphomycete species with available ITS barcodes from 119 (out of ~300 species described) to 136. Phylogenetically, the 136 species were distributed between 2 phyla, 6 classes, and 10 orders of fungi. Future studies should strive to increase the database of ITS sequences, especially focusing on species with unclear phylogenetic relationships (<i>incertae sedis</i>) and with few sequences available. The geographical distribution of species with available ITS sequences included 50 countries from five continents, but 6 countries had more than 20 species associated, showing a bias toward the northern hemisphere, likely due to sampling bias. |
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ITS rDNA barcodes clarify molecular diversity of aquatic hyphomycetesInternal transcribed spacerAquatic fungiTaxonomyMolecular identificationFreshwatersScience & TechnologyAquatic hyphomycetes are key microbial decomposers of allochthonous organic matter in freshwater ecosystems. Although their importance in carbon flow and food webs in streams is widely recognized, there are still gaps in our understanding of their molecular diversity and distribution patterns. Our study utilized the growing database of ITS rDNA barcodes of aquatic hyphomycetes (1252 sequences) and aimed to (i) produce new barcodes for some lesser-known taxa; (ii) clarify the taxonomic placement of some taxa at the class or order level, based on molecular data; and (iii) provide insights into the biogeographical origins of some taxa. This study increased the number of aquatic hyphomycete species with available ITS barcodes from 119 (out of ~300 species described) to 136. Phylogenetically, the 136 species were distributed between 2 phyla, 6 classes, and 10 orders of fungi. Future studies should strive to increase the database of ITS sequences, especially focusing on species with unclear phylogenetic relationships (<i>incertae sedis</i>) and with few sequences available. The geographical distribution of species with available ITS sequences included 50 countries from five continents, but 6 countries had more than 20 species associated, showing a bias toward the northern hemisphere, likely due to sampling bias.This work is supported by the project STREAMECO—Biodiversity and ecosystem functioning under climate change: from the gene to the stream: PTDC/CTA-AMB/31245/2017 funded by the Portuguese Foundation for Science and Technology (FCT) and by the “Contrato-Programa” UIDB/04050/2020 funded by national funds through the FCT I.P. Additional support from the National Science Foundation (NSF DEB-1655797) to V.G. is gratefully acknowledged.Multidisciplinary Digital Publishing Institute (MDPI)Universidade do MinhoFranco-Duarte, RicardoFernandes, IsabelGulis, VladislavCássio, FernandaPascoal, Cláudia2022-08-042022-08-04T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/80639engFranco-Duarte, R.; Fernandes, I.; Gulis, V.; Cássio, F.; Pascoal, C. ITS rDNA Barcodes Clarify Molecular Diversity of Aquatic Hyphomycetes. Microorganisms 2022, 10, 1569. https://doi.org/10.3390/microorganisms100815692076-260710.3390/microorganisms100815691569https://www.mdpi.com/2076-2607/10/8/1569info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:51:21Zoai:repositorium.sdum.uminho.pt:1822/80639Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:50:13.511230Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
ITS rDNA barcodes clarify molecular diversity of aquatic hyphomycetes |
title |
ITS rDNA barcodes clarify molecular diversity of aquatic hyphomycetes |
spellingShingle |
ITS rDNA barcodes clarify molecular diversity of aquatic hyphomycetes Franco-Duarte, Ricardo Internal transcribed spacer Aquatic fungi Taxonomy Molecular identification Freshwaters Science & Technology |
title_short |
ITS rDNA barcodes clarify molecular diversity of aquatic hyphomycetes |
title_full |
ITS rDNA barcodes clarify molecular diversity of aquatic hyphomycetes |
title_fullStr |
ITS rDNA barcodes clarify molecular diversity of aquatic hyphomycetes |
title_full_unstemmed |
ITS rDNA barcodes clarify molecular diversity of aquatic hyphomycetes |
title_sort |
ITS rDNA barcodes clarify molecular diversity of aquatic hyphomycetes |
author |
Franco-Duarte, Ricardo |
author_facet |
Franco-Duarte, Ricardo Fernandes, Isabel Gulis, Vladislav Cássio, Fernanda Pascoal, Cláudia |
author_role |
author |
author2 |
Fernandes, Isabel Gulis, Vladislav Cássio, Fernanda Pascoal, Cláudia |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Franco-Duarte, Ricardo Fernandes, Isabel Gulis, Vladislav Cássio, Fernanda Pascoal, Cláudia |
dc.subject.por.fl_str_mv |
Internal transcribed spacer Aquatic fungi Taxonomy Molecular identification Freshwaters Science & Technology |
topic |
Internal transcribed spacer Aquatic fungi Taxonomy Molecular identification Freshwaters Science & Technology |
description |
Aquatic hyphomycetes are key microbial decomposers of allochthonous organic matter in freshwater ecosystems. Although their importance in carbon flow and food webs in streams is widely recognized, there are still gaps in our understanding of their molecular diversity and distribution patterns. Our study utilized the growing database of ITS rDNA barcodes of aquatic hyphomycetes (1252 sequences) and aimed to (i) produce new barcodes for some lesser-known taxa; (ii) clarify the taxonomic placement of some taxa at the class or order level, based on molecular data; and (iii) provide insights into the biogeographical origins of some taxa. This study increased the number of aquatic hyphomycete species with available ITS barcodes from 119 (out of ~300 species described) to 136. Phylogenetically, the 136 species were distributed between 2 phyla, 6 classes, and 10 orders of fungi. Future studies should strive to increase the database of ITS sequences, especially focusing on species with unclear phylogenetic relationships (<i>incertae sedis</i>) and with few sequences available. The geographical distribution of species with available ITS sequences included 50 countries from five continents, but 6 countries had more than 20 species associated, showing a bias toward the northern hemisphere, likely due to sampling bias. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-08-04 2022-08-04T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/80639 |
url |
https://hdl.handle.net/1822/80639 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Franco-Duarte, R.; Fernandes, I.; Gulis, V.; Cássio, F.; Pascoal, C. ITS rDNA Barcodes Clarify Molecular Diversity of Aquatic Hyphomycetes. Microorganisms 2022, 10, 1569. https://doi.org/10.3390/microorganisms10081569 2076-2607 10.3390/microorganisms10081569 1569 https://www.mdpi.com/2076-2607/10/8/1569 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute (MDPI) |
publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute (MDPI) |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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