Inflammatory cells proliferate in the choroid and retina without choroidal thickness change in early Type 1 diabetes

Detalhes bibliográficos
Autor(a) principal: Campos, António
Data de Publicação: 2020
Outros Autores: Campos, Elisa J., Martins, João, Rodrigues, Flávia S.C., Silva, Rufino, Ambrósio, António Francisco
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.8/6658
Resumo: Increasing evidence points to inflammation as a key factor in the pathogenesis of diabetic retinopathy (DR). Choroidal inflammatory changes in diabetes have been reported and in vivo choroidal thickness (CT) has been searched as a marker of retinopathy with contradictory results. We aimed to investigate the early stages in the retina and choroid in an animal model of Type 1 diabetes. Type 1 diabetes was induced in male Wistar rats via a single i.p. streptozotocin injection. At 8 weeks after disease onset, CT, choroidal vascular density, VEGF and VEGFR2 expression, microglial cell and pericyte distribution were evaluated. Diabetic rats showed no significant change in CT and choroidal vascular density. A widened pericyte-free gap between the retinal pigment epithelium and the choroid was observed in diabetic rats. The immunoreactivity of VEGFR2 was decreased in the retina of diabetic rats, despite no statistically significant difference in the immunoreactivity of VEGF. The density of microglial cells significantly increased in the choroid and retina of diabetic rats. Reactive microglial cells were found to be more abundant in the choroid of diabetic rats. Evidences of the interconnection between the superficial, intermediate, and deep plexuses of the retina were also observed. At early stages, Type 1 diabetes does not affect choroidal thickness and choroidal vascular density. Proliferation and reactivity of microglial cells occurs in the choroidal stroma and the retina. The expression of VEGFR2 decreases in the retina.
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spelling Inflammatory cells proliferate in the choroid and retina without choroidal thickness change in early Type 1 diabetesChoroidRetinaType 1 diabetesChoroidal thicknessMicrogliaIncreasing evidence points to inflammation as a key factor in the pathogenesis of diabetic retinopathy (DR). Choroidal inflammatory changes in diabetes have been reported and in vivo choroidal thickness (CT) has been searched as a marker of retinopathy with contradictory results. We aimed to investigate the early stages in the retina and choroid in an animal model of Type 1 diabetes. Type 1 diabetes was induced in male Wistar rats via a single i.p. streptozotocin injection. At 8 weeks after disease onset, CT, choroidal vascular density, VEGF and VEGFR2 expression, microglial cell and pericyte distribution were evaluated. Diabetic rats showed no significant change in CT and choroidal vascular density. A widened pericyte-free gap between the retinal pigment epithelium and the choroid was observed in diabetic rats. The immunoreactivity of VEGFR2 was decreased in the retina of diabetic rats, despite no statistically significant difference in the immunoreactivity of VEGF. The density of microglial cells significantly increased in the choroid and retina of diabetic rats. Reactive microglial cells were found to be more abundant in the choroid of diabetic rats. Evidences of the interconnection between the superficial, intermediate, and deep plexuses of the retina were also observed. At early stages, Type 1 diabetes does not affect choroidal thickness and choroidal vascular density. Proliferation and reactivity of microglial cells occurs in the choroidal stroma and the retina. The expression of VEGFR2 decreases in the retina.ElsevierIC-OnlineCampos, AntónioCampos, Elisa J.Martins, JoãoRodrigues, Flávia S.C.Silva, RufinoAmbrósio, António Francisco2022-02-11T14:51:49Z2020-08-222020-08-22T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.8/6658engCampos, A., Campos, E. J., Martins, J., Rodrigues, F. S. C., Silva, R., & Ambrósio, A. F. (2020). Inflammatory cells proliferate in the choroid and retina without choroidal thickness change in early Type 1 diabetes. Experimental Eye Research, 199, 108195. https://doi.org/10.1016/j.exer.2020.1081950014-483510.1016/j.exer.2020.108195metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-17T15:53:23Zoai:iconline.ipleiria.pt:10400.8/6658Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:49:45.862836Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Inflammatory cells proliferate in the choroid and retina without choroidal thickness change in early Type 1 diabetes
title Inflammatory cells proliferate in the choroid and retina without choroidal thickness change in early Type 1 diabetes
spellingShingle Inflammatory cells proliferate in the choroid and retina without choroidal thickness change in early Type 1 diabetes
Campos, António
Choroid
Retina
Type 1 diabetes
Choroidal thickness
Microglia
title_short Inflammatory cells proliferate in the choroid and retina without choroidal thickness change in early Type 1 diabetes
title_full Inflammatory cells proliferate in the choroid and retina without choroidal thickness change in early Type 1 diabetes
title_fullStr Inflammatory cells proliferate in the choroid and retina without choroidal thickness change in early Type 1 diabetes
title_full_unstemmed Inflammatory cells proliferate in the choroid and retina without choroidal thickness change in early Type 1 diabetes
title_sort Inflammatory cells proliferate in the choroid and retina without choroidal thickness change in early Type 1 diabetes
author Campos, António
author_facet Campos, António
Campos, Elisa J.
Martins, João
Rodrigues, Flávia S.C.
Silva, Rufino
Ambrósio, António Francisco
author_role author
author2 Campos, Elisa J.
Martins, João
Rodrigues, Flávia S.C.
Silva, Rufino
Ambrósio, António Francisco
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv IC-Online
dc.contributor.author.fl_str_mv Campos, António
Campos, Elisa J.
Martins, João
Rodrigues, Flávia S.C.
Silva, Rufino
Ambrósio, António Francisco
dc.subject.por.fl_str_mv Choroid
Retina
Type 1 diabetes
Choroidal thickness
Microglia
topic Choroid
Retina
Type 1 diabetes
Choroidal thickness
Microglia
description Increasing evidence points to inflammation as a key factor in the pathogenesis of diabetic retinopathy (DR). Choroidal inflammatory changes in diabetes have been reported and in vivo choroidal thickness (CT) has been searched as a marker of retinopathy with contradictory results. We aimed to investigate the early stages in the retina and choroid in an animal model of Type 1 diabetes. Type 1 diabetes was induced in male Wistar rats via a single i.p. streptozotocin injection. At 8 weeks after disease onset, CT, choroidal vascular density, VEGF and VEGFR2 expression, microglial cell and pericyte distribution were evaluated. Diabetic rats showed no significant change in CT and choroidal vascular density. A widened pericyte-free gap between the retinal pigment epithelium and the choroid was observed in diabetic rats. The immunoreactivity of VEGFR2 was decreased in the retina of diabetic rats, despite no statistically significant difference in the immunoreactivity of VEGF. The density of microglial cells significantly increased in the choroid and retina of diabetic rats. Reactive microglial cells were found to be more abundant in the choroid of diabetic rats. Evidences of the interconnection between the superficial, intermediate, and deep plexuses of the retina were also observed. At early stages, Type 1 diabetes does not affect choroidal thickness and choroidal vascular density. Proliferation and reactivity of microglial cells occurs in the choroidal stroma and the retina. The expression of VEGFR2 decreases in the retina.
publishDate 2020
dc.date.none.fl_str_mv 2020-08-22
2020-08-22T00:00:00Z
2022-02-11T14:51:49Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.8/6658
url http://hdl.handle.net/10400.8/6658
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Campos, A., Campos, E. J., Martins, J., Rodrigues, F. S. C., Silva, R., & Ambrósio, A. F. (2020). Inflammatory cells proliferate in the choroid and retina without choroidal thickness change in early Type 1 diabetes. Experimental Eye Research, 199, 108195. https://doi.org/10.1016/j.exer.2020.108195
0014-4835
10.1016/j.exer.2020.108195
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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