Employing bacteria machinery for antibiotic detection: Using DNA gyrase for ciprofloxacin detection

Detalhes bibliográficos
Autor(a) principal: Cardoso, Ana Rita
Data de Publicação: 2021
Outros Autores: Carneiro, Liliana P.T., Cabral-Miranda, Gustavo, Bachmann, Martin F., Sales, Maria Goreti Ferreira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.22/18564
Resumo: This work describes a new successful approach for designing biosensors that detect antibiotics. It makes use of a biomimetic strategy, by employing the biochemical target of a given antibiotic as its biorecognition element. This principle was tested herein for quinolones, which target DNA gyrase in bacteria. Ciprofloxacin (CIPRO) was tested as a representative antibiotic from the quinolone group; the sensitivity of biosensor to this group was confirmed by checking the response to another quinolone antibiotic (norfloxacin, NOR) and to a non-quinolone antibiotic (ampicillin, AMP). The biorecognition element used was DNA gyrase attached by ionic interactions to a carbon support, on a working electrode on common screen-printed electrodes (SPEs). The response against antibiotics was tested for increasing concentrations of CIPRO, NOR or AMP, and following the subsequent electrical changes by electrochemical impedance spectroscopy. The DNAgyrase biosensor showed sensitive responses for CIPRO and NOR, for concentrations down to 3.02 nM and 30.2 nM, respectively, with a very wide response range for CRIPRO, up to 30.2 µM. Its response was also confirmed selective for quinolones, when compared to its response against AMP. Further comparison to an immunosensor of similar design (adding antibodies instead of DNA gyrase) was made, revealing favourable features for the new biomimetic biosensor with 1.52 nM of limit of detection (LOD). Overall, the new approach presented herein is simple and effective for antibiotic detection, displaying a selective response against a given antibiotic group. The use of bacterial machinery as biorecognition element in biosensors may also provide a valuable tool to study the mechanism of action in bacterial cells of new drugs. This is especially important in the development of new drugs to fight bacterial resistance.
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spelling Employing bacteria machinery for antibiotic detection: Using DNA gyrase for ciprofloxacin detectionBiochemical target of antibioticsDNA gyraseCiprofloxacinScreen-printed electrodesElectrochemical biosensorAntibodiesThis work describes a new successful approach for designing biosensors that detect antibiotics. It makes use of a biomimetic strategy, by employing the biochemical target of a given antibiotic as its biorecognition element. This principle was tested herein for quinolones, which target DNA gyrase in bacteria. Ciprofloxacin (CIPRO) was tested as a representative antibiotic from the quinolone group; the sensitivity of biosensor to this group was confirmed by checking the response to another quinolone antibiotic (norfloxacin, NOR) and to a non-quinolone antibiotic (ampicillin, AMP). The biorecognition element used was DNA gyrase attached by ionic interactions to a carbon support, on a working electrode on common screen-printed electrodes (SPEs). The response against antibiotics was tested for increasing concentrations of CIPRO, NOR or AMP, and following the subsequent electrical changes by electrochemical impedance spectroscopy. The DNAgyrase biosensor showed sensitive responses for CIPRO and NOR, for concentrations down to 3.02 nM and 30.2 nM, respectively, with a very wide response range for CRIPRO, up to 30.2 µM. Its response was also confirmed selective for quinolones, when compared to its response against AMP. Further comparison to an immunosensor of similar design (adding antibodies instead of DNA gyrase) was made, revealing favourable features for the new biomimetic biosensor with 1.52 nM of limit of detection (LOD). Overall, the new approach presented herein is simple and effective for antibiotic detection, displaying a selective response against a given antibiotic group. The use of bacterial machinery as biorecognition element in biosensors may also provide a valuable tool to study the mechanism of action in bacterial cells of new drugs. This is especially important in the development of new drugs to fight bacterial resistance.The authors acknowledge funding from project PTDC/AAG-TEC/5400/2014 funded by European funds, through FEDER (European Funding or Regional Development) via COMPETE2020 – POCI (operational program for internationalization and competitively) and by national funding through the National Foundation for Science and Technology, I.P. (FCT). ARC also acknowledge funding to National Foundation for Science and Technology, I.P., through the PhD Grant, SFRH/BD/130107/2017.ElsevierRepositório Científico do Instituto Politécnico do PortoCardoso, Ana RitaCarneiro, Liliana P.T.Cabral-Miranda, GustavoBachmann, Martin F.Sales, Maria Goreti Ferreira20212031-01-01T00:00:00Z2021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfapplication/pdfhttp://hdl.handle.net/10400.22/18564eng10.1016/j.cej.2020.128135info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T13:10:22Zoai:recipp.ipp.pt:10400.22/18564Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:38:08.871455Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Employing bacteria machinery for antibiotic detection: Using DNA gyrase for ciprofloxacin detection
title Employing bacteria machinery for antibiotic detection: Using DNA gyrase for ciprofloxacin detection
spellingShingle Employing bacteria machinery for antibiotic detection: Using DNA gyrase for ciprofloxacin detection
Cardoso, Ana Rita
Biochemical target of antibiotics
DNA gyrase
Ciprofloxacin
Screen-printed electrodes
Electrochemical biosensor
Antibodies
title_short Employing bacteria machinery for antibiotic detection: Using DNA gyrase for ciprofloxacin detection
title_full Employing bacteria machinery for antibiotic detection: Using DNA gyrase for ciprofloxacin detection
title_fullStr Employing bacteria machinery for antibiotic detection: Using DNA gyrase for ciprofloxacin detection
title_full_unstemmed Employing bacteria machinery for antibiotic detection: Using DNA gyrase for ciprofloxacin detection
title_sort Employing bacteria machinery for antibiotic detection: Using DNA gyrase for ciprofloxacin detection
author Cardoso, Ana Rita
author_facet Cardoso, Ana Rita
Carneiro, Liliana P.T.
Cabral-Miranda, Gustavo
Bachmann, Martin F.
Sales, Maria Goreti Ferreira
author_role author
author2 Carneiro, Liliana P.T.
Cabral-Miranda, Gustavo
Bachmann, Martin F.
Sales, Maria Goreti Ferreira
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Politécnico do Porto
dc.contributor.author.fl_str_mv Cardoso, Ana Rita
Carneiro, Liliana P.T.
Cabral-Miranda, Gustavo
Bachmann, Martin F.
Sales, Maria Goreti Ferreira
dc.subject.por.fl_str_mv Biochemical target of antibiotics
DNA gyrase
Ciprofloxacin
Screen-printed electrodes
Electrochemical biosensor
Antibodies
topic Biochemical target of antibiotics
DNA gyrase
Ciprofloxacin
Screen-printed electrodes
Electrochemical biosensor
Antibodies
description This work describes a new successful approach for designing biosensors that detect antibiotics. It makes use of a biomimetic strategy, by employing the biochemical target of a given antibiotic as its biorecognition element. This principle was tested herein for quinolones, which target DNA gyrase in bacteria. Ciprofloxacin (CIPRO) was tested as a representative antibiotic from the quinolone group; the sensitivity of biosensor to this group was confirmed by checking the response to another quinolone antibiotic (norfloxacin, NOR) and to a non-quinolone antibiotic (ampicillin, AMP). The biorecognition element used was DNA gyrase attached by ionic interactions to a carbon support, on a working electrode on common screen-printed electrodes (SPEs). The response against antibiotics was tested for increasing concentrations of CIPRO, NOR or AMP, and following the subsequent electrical changes by electrochemical impedance spectroscopy. The DNAgyrase biosensor showed sensitive responses for CIPRO and NOR, for concentrations down to 3.02 nM and 30.2 nM, respectively, with a very wide response range for CRIPRO, up to 30.2 µM. Its response was also confirmed selective for quinolones, when compared to its response against AMP. Further comparison to an immunosensor of similar design (adding antibodies instead of DNA gyrase) was made, revealing favourable features for the new biomimetic biosensor with 1.52 nM of limit of detection (LOD). Overall, the new approach presented herein is simple and effective for antibiotic detection, displaying a selective response against a given antibiotic group. The use of bacterial machinery as biorecognition element in biosensors may also provide a valuable tool to study the mechanism of action in bacterial cells of new drugs. This is especially important in the development of new drugs to fight bacterial resistance.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-01-01T00:00:00Z
2031-01-01T00:00:00Z
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.22/18564
url http://hdl.handle.net/10400.22/18564
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1016/j.cej.2020.128135
dc.rights.driver.fl_str_mv info:eu-repo/semantics/embargoedAccess
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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