Carvedilol: Relação Entre A Actividade Antioxidante e Inibição da Transição de Permeabilidade Mitocondrial

Detalhes bibliográficos
Autor(a) principal: Oliveira, PJ
Data de Publicação: 2003
Outros Autores: Esteves, T, Rolo, AP, Monteiro, P, Gonçalves, L, Palmeira, CM, Moreno, AJ
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.4/339
Resumo: OBJECTIVES: The mitochondrial permeability transition (MPT) is an event related to severe oxidative stress (for example, during myocardial ischemia and reperfusion) and excessive mitochondrial calcium accumulation, also being implicated in cell death. In this study, we compared the effect of carvedilol on the cardiac MPT induced by calcium and phosphate (Ca/Pi) and calcium/carboxyatractyloside (Ca/Catr). Oxidative stress plays a major role in MPT induction by Ca/Pi, leading to the oxidation of protein thiol groups, in contrast with Ca/Catr, where such oxidation is secondary to MPT induction and is not caused by oxidative stress. MATERIALS AND METHODS: Mitochondria were isolated from rat hearts and parameters related to MPT induction were evaluated (n = 5 for each inducer): mitochondrial swelling and oxidation of protein thiol groups (both measured by spectrophotometry). RESULTS: Using Ca/Pi, carvedilol protected mitochondria from MPT induction, particularly in its high conductance form. Its effect was demonstrated by analyzing the decrease in mitochondrial swelling amplitude. Simultaneously, we observed inhibition of protein thiol group oxidation (p < 0.001). By contrast, carvedilol did not show any protective effect with Ca/Catr. CONCLUSIONS: Carvedilol was only effective against the MPT when the oxidation of protein thiol groups was the cause and not the consequence of the MPT phenomenon. The results clearly show that during myocardial aggressions (ischemia and reperfusion, for example), the protective effect of carvedilol is primarily due to an antioxidant mechanism, inhibiting the production and effects of reactive oxygen species.
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spelling Carvedilol: Relação Entre A Actividade Antioxidante e Inibição da Transição de Permeabilidade MitocondrialCarvedilol: relation between antioxidant activity and inhibition of the mitochondrial permeability transitionAntioxidantesMitocôndrias CardiacasMembranas IntracelularesOBJECTIVES: The mitochondrial permeability transition (MPT) is an event related to severe oxidative stress (for example, during myocardial ischemia and reperfusion) and excessive mitochondrial calcium accumulation, also being implicated in cell death. In this study, we compared the effect of carvedilol on the cardiac MPT induced by calcium and phosphate (Ca/Pi) and calcium/carboxyatractyloside (Ca/Catr). Oxidative stress plays a major role in MPT induction by Ca/Pi, leading to the oxidation of protein thiol groups, in contrast with Ca/Catr, where such oxidation is secondary to MPT induction and is not caused by oxidative stress. MATERIALS AND METHODS: Mitochondria were isolated from rat hearts and parameters related to MPT induction were evaluated (n = 5 for each inducer): mitochondrial swelling and oxidation of protein thiol groups (both measured by spectrophotometry). RESULTS: Using Ca/Pi, carvedilol protected mitochondria from MPT induction, particularly in its high conductance form. Its effect was demonstrated by analyzing the decrease in mitochondrial swelling amplitude. Simultaneously, we observed inhibition of protein thiol group oxidation (p < 0.001). By contrast, carvedilol did not show any protective effect with Ca/Catr. CONCLUSIONS: Carvedilol was only effective against the MPT when the oxidation of protein thiol groups was the cause and not the consequence of the MPT phenomenon. The results clearly show that during myocardial aggressions (ischemia and reperfusion, for example), the protective effect of carvedilol is primarily due to an antioxidant mechanism, inhibiting the production and effects of reactive oxygen species.Sociedade Portuguesa de CardiologiaRIHUCOliveira, PJEsteves, TRolo, APMonteiro, PGonçalves, LPalmeira, CMMoreno, AJ2008-12-12T14:59:14Z20032003-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.4/339porRev Port Cardiol. 2003 Jan;22(1):55-62info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-11T14:21:30Zoai:rihuc.huc.min-saude.pt:10400.4/339Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:03:08.901490Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Carvedilol: Relação Entre A Actividade Antioxidante e Inibição da Transição de Permeabilidade Mitocondrial
Carvedilol: relation between antioxidant activity and inhibition of the mitochondrial permeability transition
title Carvedilol: Relação Entre A Actividade Antioxidante e Inibição da Transição de Permeabilidade Mitocondrial
spellingShingle Carvedilol: Relação Entre A Actividade Antioxidante e Inibição da Transição de Permeabilidade Mitocondrial
Oliveira, PJ
Antioxidantes
Mitocôndrias Cardiacas
Membranas Intracelulares
title_short Carvedilol: Relação Entre A Actividade Antioxidante e Inibição da Transição de Permeabilidade Mitocondrial
title_full Carvedilol: Relação Entre A Actividade Antioxidante e Inibição da Transição de Permeabilidade Mitocondrial
title_fullStr Carvedilol: Relação Entre A Actividade Antioxidante e Inibição da Transição de Permeabilidade Mitocondrial
title_full_unstemmed Carvedilol: Relação Entre A Actividade Antioxidante e Inibição da Transição de Permeabilidade Mitocondrial
title_sort Carvedilol: Relação Entre A Actividade Antioxidante e Inibição da Transição de Permeabilidade Mitocondrial
author Oliveira, PJ
author_facet Oliveira, PJ
Esteves, T
Rolo, AP
Monteiro, P
Gonçalves, L
Palmeira, CM
Moreno, AJ
author_role author
author2 Esteves, T
Rolo, AP
Monteiro, P
Gonçalves, L
Palmeira, CM
Moreno, AJ
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv RIHUC
dc.contributor.author.fl_str_mv Oliveira, PJ
Esteves, T
Rolo, AP
Monteiro, P
Gonçalves, L
Palmeira, CM
Moreno, AJ
dc.subject.por.fl_str_mv Antioxidantes
Mitocôndrias Cardiacas
Membranas Intracelulares
topic Antioxidantes
Mitocôndrias Cardiacas
Membranas Intracelulares
description OBJECTIVES: The mitochondrial permeability transition (MPT) is an event related to severe oxidative stress (for example, during myocardial ischemia and reperfusion) and excessive mitochondrial calcium accumulation, also being implicated in cell death. In this study, we compared the effect of carvedilol on the cardiac MPT induced by calcium and phosphate (Ca/Pi) and calcium/carboxyatractyloside (Ca/Catr). Oxidative stress plays a major role in MPT induction by Ca/Pi, leading to the oxidation of protein thiol groups, in contrast with Ca/Catr, where such oxidation is secondary to MPT induction and is not caused by oxidative stress. MATERIALS AND METHODS: Mitochondria were isolated from rat hearts and parameters related to MPT induction were evaluated (n = 5 for each inducer): mitochondrial swelling and oxidation of protein thiol groups (both measured by spectrophotometry). RESULTS: Using Ca/Pi, carvedilol protected mitochondria from MPT induction, particularly in its high conductance form. Its effect was demonstrated by analyzing the decrease in mitochondrial swelling amplitude. Simultaneously, we observed inhibition of protein thiol group oxidation (p < 0.001). By contrast, carvedilol did not show any protective effect with Ca/Catr. CONCLUSIONS: Carvedilol was only effective against the MPT when the oxidation of protein thiol groups was the cause and not the consequence of the MPT phenomenon. The results clearly show that during myocardial aggressions (ischemia and reperfusion, for example), the protective effect of carvedilol is primarily due to an antioxidant mechanism, inhibiting the production and effects of reactive oxygen species.
publishDate 2003
dc.date.none.fl_str_mv 2003
2003-01-01T00:00:00Z
2008-12-12T14:59:14Z
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.4/339
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dc.relation.none.fl_str_mv Rev Port Cardiol. 2003 Jan;22(1):55-62
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dc.publisher.none.fl_str_mv Sociedade Portuguesa de Cardiologia
publisher.none.fl_str_mv Sociedade Portuguesa de Cardiologia
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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