Impacto do Carvedilol Sobre O Dano Mitocondrial Induzido Por Hipoxantina/xantina Oxidase: Que Papel Na Isquemia e Reperfusão do Miocárdio?
Autor(a) principal: | |
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Data de Publicação: | 2002 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.4/340 |
Resumo: | OBJECTIVES: The cardioprotective effects of carvedilol (CV) may be explained in part by interactions with heart mitochondria. The objective of this work was to study the protection afforded by CV against oxidative stress induced in isolated heart mitochondria by hypoxanthine and xanthine oxidase (HX/XO), a well-known source of reactive oxygen species (ROS) in the cardiovascular system. METHODS: Mitochondria were isolated from Wistar rat hearts (n = 8) and incubated with HX/XO in the presence and in the absence of calcium. Several methods were used to assess the protection afforded by CV: evaluation of mitochondrial volume changes (by measuring changes in the optical density of the mitochondrial suspension), calcium uptake and release (with a fluorescent probe, Calcium Green 5-N) and mitochondrial respiration (with a Clark-type oxygen electrode). RESULTS: CV decreased mitochondrial damage associated with ROS production by HX and XO, as verified by the reduction of mitochondrial swelling and increase in mitochondrial calcium uptake. In the presence of HX and XO, CV also ameliorated mitochondrial respiration in the active phosphorylation state and prevented decrease in the respiratory control ratio (p < 0.05) and in mitochondrial phosphorylative efficiency (p < 0.001). CONCLUSIONS: The data indicate that CV partly protected heart mitochondria from oxidative damage induced by HX and XO, which may be useful during myocardial ischemia and reperfusion. It is also suggested that mitochondria may be a priority target for the protective action of some compounds. |
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7160 |
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Impacto do Carvedilol Sobre O Dano Mitocondrial Induzido Por Hipoxantina/xantina Oxidase: Que Papel Na Isquemia e Reperfusão do Miocárdio?Impact of carvedilol on the mitochondrial damage induced by hypoxanthine and xantine oxidase: what role in myocardial ischemia and reperfusion?AntioxidantesIsquemia do MiocárdioMitocôndrias CardiacasPropanolaminaOBJECTIVES: The cardioprotective effects of carvedilol (CV) may be explained in part by interactions with heart mitochondria. The objective of this work was to study the protection afforded by CV against oxidative stress induced in isolated heart mitochondria by hypoxanthine and xanthine oxidase (HX/XO), a well-known source of reactive oxygen species (ROS) in the cardiovascular system. METHODS: Mitochondria were isolated from Wistar rat hearts (n = 8) and incubated with HX/XO in the presence and in the absence of calcium. Several methods were used to assess the protection afforded by CV: evaluation of mitochondrial volume changes (by measuring changes in the optical density of the mitochondrial suspension), calcium uptake and release (with a fluorescent probe, Calcium Green 5-N) and mitochondrial respiration (with a Clark-type oxygen electrode). RESULTS: CV decreased mitochondrial damage associated with ROS production by HX and XO, as verified by the reduction of mitochondrial swelling and increase in mitochondrial calcium uptake. In the presence of HX and XO, CV also ameliorated mitochondrial respiration in the active phosphorylation state and prevented decrease in the respiratory control ratio (p < 0.05) and in mitochondrial phosphorylative efficiency (p < 0.001). CONCLUSIONS: The data indicate that CV partly protected heart mitochondria from oxidative damage induced by HX and XO, which may be useful during myocardial ischemia and reperfusion. It is also suggested that mitochondria may be a priority target for the protective action of some compounds.Sociedade Portuguesa de CardiologiaRIHUCOliveira, PJRolo, APMonteiro, PGonçalves, LPalmeira, CMMoreno, AJ2008-12-12T14:59:23Z20022002-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.4/340porRev Port Cardiol. 2002 Dec;21(12):1447-55info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-11T14:21:30Zoai:rihuc.huc.min-saude.pt:10400.4/340Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:03:08.957675Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Impacto do Carvedilol Sobre O Dano Mitocondrial Induzido Por Hipoxantina/xantina Oxidase: Que Papel Na Isquemia e Reperfusão do Miocárdio? Impact of carvedilol on the mitochondrial damage induced by hypoxanthine and xantine oxidase: what role in myocardial ischemia and reperfusion? |
title |
Impacto do Carvedilol Sobre O Dano Mitocondrial Induzido Por Hipoxantina/xantina Oxidase: Que Papel Na Isquemia e Reperfusão do Miocárdio? |
spellingShingle |
Impacto do Carvedilol Sobre O Dano Mitocondrial Induzido Por Hipoxantina/xantina Oxidase: Que Papel Na Isquemia e Reperfusão do Miocárdio? Oliveira, PJ Antioxidantes Isquemia do Miocárdio Mitocôndrias Cardiacas Propanolamina |
title_short |
Impacto do Carvedilol Sobre O Dano Mitocondrial Induzido Por Hipoxantina/xantina Oxidase: Que Papel Na Isquemia e Reperfusão do Miocárdio? |
title_full |
Impacto do Carvedilol Sobre O Dano Mitocondrial Induzido Por Hipoxantina/xantina Oxidase: Que Papel Na Isquemia e Reperfusão do Miocárdio? |
title_fullStr |
Impacto do Carvedilol Sobre O Dano Mitocondrial Induzido Por Hipoxantina/xantina Oxidase: Que Papel Na Isquemia e Reperfusão do Miocárdio? |
title_full_unstemmed |
Impacto do Carvedilol Sobre O Dano Mitocondrial Induzido Por Hipoxantina/xantina Oxidase: Que Papel Na Isquemia e Reperfusão do Miocárdio? |
title_sort |
Impacto do Carvedilol Sobre O Dano Mitocondrial Induzido Por Hipoxantina/xantina Oxidase: Que Papel Na Isquemia e Reperfusão do Miocárdio? |
author |
Oliveira, PJ |
author_facet |
Oliveira, PJ Rolo, AP Monteiro, P Gonçalves, L Palmeira, CM Moreno, AJ |
author_role |
author |
author2 |
Rolo, AP Monteiro, P Gonçalves, L Palmeira, CM Moreno, AJ |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
RIHUC |
dc.contributor.author.fl_str_mv |
Oliveira, PJ Rolo, AP Monteiro, P Gonçalves, L Palmeira, CM Moreno, AJ |
dc.subject.por.fl_str_mv |
Antioxidantes Isquemia do Miocárdio Mitocôndrias Cardiacas Propanolamina |
topic |
Antioxidantes Isquemia do Miocárdio Mitocôndrias Cardiacas Propanolamina |
description |
OBJECTIVES: The cardioprotective effects of carvedilol (CV) may be explained in part by interactions with heart mitochondria. The objective of this work was to study the protection afforded by CV against oxidative stress induced in isolated heart mitochondria by hypoxanthine and xanthine oxidase (HX/XO), a well-known source of reactive oxygen species (ROS) in the cardiovascular system. METHODS: Mitochondria were isolated from Wistar rat hearts (n = 8) and incubated with HX/XO in the presence and in the absence of calcium. Several methods were used to assess the protection afforded by CV: evaluation of mitochondrial volume changes (by measuring changes in the optical density of the mitochondrial suspension), calcium uptake and release (with a fluorescent probe, Calcium Green 5-N) and mitochondrial respiration (with a Clark-type oxygen electrode). RESULTS: CV decreased mitochondrial damage associated with ROS production by HX and XO, as verified by the reduction of mitochondrial swelling and increase in mitochondrial calcium uptake. In the presence of HX and XO, CV also ameliorated mitochondrial respiration in the active phosphorylation state and prevented decrease in the respiratory control ratio (p < 0.05) and in mitochondrial phosphorylative efficiency (p < 0.001). CONCLUSIONS: The data indicate that CV partly protected heart mitochondria from oxidative damage induced by HX and XO, which may be useful during myocardial ischemia and reperfusion. It is also suggested that mitochondria may be a priority target for the protective action of some compounds. |
publishDate |
2002 |
dc.date.none.fl_str_mv |
2002 2002-01-01T00:00:00Z 2008-12-12T14:59:23Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.4/340 |
url |
http://hdl.handle.net/10400.4/340 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Rev Port Cardiol. 2002 Dec;21(12):1447-55 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Portuguesa de Cardiologia |
publisher.none.fl_str_mv |
Sociedade Portuguesa de Cardiologia |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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