Urinary volatilomics unveils a candidate biomarker panel for noninvasive detection of clear cell renal cell carcinoma
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10284/9977 |
Resumo: | Clear cell renal cell carcinoma (ccRCC) is the most common type of kidney cancer usually associated with asymptomatic development and risk of systemic progression. Hence, reliable molecular biomarkers of ccRCC are needed to provide early and minimally invasive detection. In this study, urinary volatilome profiling of patients diagnosed with ccRCC (n = 75), and cancer-free controls (n = 75), was performed to investigate the presence of a volatile signature characteristic of ccRCC. Volatile organic compounds (VOCs) in general, and more specifically volatile carbonyl compounds (VCCs), present in urine were extracted by headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME-GC-MS). Supervised multivariate models showed a good discriminatory power of ccRCC patients from controls in urine. Overall, 22 volatile metabolites were found significantly altered between the two groups, including aldehydes, ketones, aromatic hydrocarbons, and terpenoids. A candidate six-biomarker panel, comprising octanal, 3-methylbutanal, benzaldehyde, 2-furaldehyde, 4-heptanone, and p-cresol, depicted the best performance for ccRCC detection with 83% sensitivity, 79% specificity, and 81% accuracy. Moreover, the ccRCC urinary volatilome signature suggested dysregulation of energy metabolism and overexpression of enzymes associated with carcinogenesis. These findings provide the molecular basis for the fine-tuning of gas-sensing materials for application in the development of a bioelectronic sensor. |
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Urinary volatilomics unveils a candidate biomarker panel for noninvasive detection of clear cell renal cell carcinomaClear cell renal cell carcinomaDiagnostic biomarkersUrineVolatilomeGas chromatography - mass spectrometryClear cell renal cell carcinoma (ccRCC) is the most common type of kidney cancer usually associated with asymptomatic development and risk of systemic progression. Hence, reliable molecular biomarkers of ccRCC are needed to provide early and minimally invasive detection. In this study, urinary volatilome profiling of patients diagnosed with ccRCC (n = 75), and cancer-free controls (n = 75), was performed to investigate the presence of a volatile signature characteristic of ccRCC. Volatile organic compounds (VOCs) in general, and more specifically volatile carbonyl compounds (VCCs), present in urine were extracted by headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME-GC-MS). Supervised multivariate models showed a good discriminatory power of ccRCC patients from controls in urine. Overall, 22 volatile metabolites were found significantly altered between the two groups, including aldehydes, ketones, aromatic hydrocarbons, and terpenoids. A candidate six-biomarker panel, comprising octanal, 3-methylbutanal, benzaldehyde, 2-furaldehyde, 4-heptanone, and p-cresol, depicted the best performance for ccRCC detection with 83% sensitivity, 79% specificity, and 81% accuracy. Moreover, the ccRCC urinary volatilome signature suggested dysregulation of energy metabolism and overexpression of enzymes associated with carcinogenesis. These findings provide the molecular basis for the fine-tuning of gas-sensing materials for application in the development of a bioelectronic sensor.ACS PublicationsRepositório Institucional da Universidade Fernando PessoaPinto, JoanaAmaro, FilipaLima, Ana RitaCarvalho-Maia, CarinaJerónimo, CarmenHenrique, RuiBastos, Maria de LourdesCarvalho, MárciaGuedes de Pinho, Paula2021-06-30T09:42:37Z2021-01-01T00:00:00Z2021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10284/9977eng1535-389310.1021/acs.jproteome.0c009361535-3907metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-09-06T02:09:16Zoai:bdigital.ufp.pt:10284/9977Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:46:45.385962Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Urinary volatilomics unveils a candidate biomarker panel for noninvasive detection of clear cell renal cell carcinoma |
title |
Urinary volatilomics unveils a candidate biomarker panel for noninvasive detection of clear cell renal cell carcinoma |
spellingShingle |
Urinary volatilomics unveils a candidate biomarker panel for noninvasive detection of clear cell renal cell carcinoma Pinto, Joana Clear cell renal cell carcinoma Diagnostic biomarkers Urine Volatilome Gas chromatography - mass spectrometry |
title_short |
Urinary volatilomics unveils a candidate biomarker panel for noninvasive detection of clear cell renal cell carcinoma |
title_full |
Urinary volatilomics unveils a candidate biomarker panel for noninvasive detection of clear cell renal cell carcinoma |
title_fullStr |
Urinary volatilomics unveils a candidate biomarker panel for noninvasive detection of clear cell renal cell carcinoma |
title_full_unstemmed |
Urinary volatilomics unveils a candidate biomarker panel for noninvasive detection of clear cell renal cell carcinoma |
title_sort |
Urinary volatilomics unveils a candidate biomarker panel for noninvasive detection of clear cell renal cell carcinoma |
author |
Pinto, Joana |
author_facet |
Pinto, Joana Amaro, Filipa Lima, Ana Rita Carvalho-Maia, Carina Jerónimo, Carmen Henrique, Rui Bastos, Maria de Lourdes Carvalho, Márcia Guedes de Pinho, Paula |
author_role |
author |
author2 |
Amaro, Filipa Lima, Ana Rita Carvalho-Maia, Carina Jerónimo, Carmen Henrique, Rui Bastos, Maria de Lourdes Carvalho, Márcia Guedes de Pinho, Paula |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Institucional da Universidade Fernando Pessoa |
dc.contributor.author.fl_str_mv |
Pinto, Joana Amaro, Filipa Lima, Ana Rita Carvalho-Maia, Carina Jerónimo, Carmen Henrique, Rui Bastos, Maria de Lourdes Carvalho, Márcia Guedes de Pinho, Paula |
dc.subject.por.fl_str_mv |
Clear cell renal cell carcinoma Diagnostic biomarkers Urine Volatilome Gas chromatography - mass spectrometry |
topic |
Clear cell renal cell carcinoma Diagnostic biomarkers Urine Volatilome Gas chromatography - mass spectrometry |
description |
Clear cell renal cell carcinoma (ccRCC) is the most common type of kidney cancer usually associated with asymptomatic development and risk of systemic progression. Hence, reliable molecular biomarkers of ccRCC are needed to provide early and minimally invasive detection. In this study, urinary volatilome profiling of patients diagnosed with ccRCC (n = 75), and cancer-free controls (n = 75), was performed to investigate the presence of a volatile signature characteristic of ccRCC. Volatile organic compounds (VOCs) in general, and more specifically volatile carbonyl compounds (VCCs), present in urine were extracted by headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME-GC-MS). Supervised multivariate models showed a good discriminatory power of ccRCC patients from controls in urine. Overall, 22 volatile metabolites were found significantly altered between the two groups, including aldehydes, ketones, aromatic hydrocarbons, and terpenoids. A candidate six-biomarker panel, comprising octanal, 3-methylbutanal, benzaldehyde, 2-furaldehyde, 4-heptanone, and p-cresol, depicted the best performance for ccRCC detection with 83% sensitivity, 79% specificity, and 81% accuracy. Moreover, the ccRCC urinary volatilome signature suggested dysregulation of energy metabolism and overexpression of enzymes associated with carcinogenesis. These findings provide the molecular basis for the fine-tuning of gas-sensing materials for application in the development of a bioelectronic sensor. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-30T09:42:37Z 2021-01-01T00:00:00Z 2021-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10284/9977 |
url |
http://hdl.handle.net/10284/9977 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1535-3893 10.1021/acs.jproteome.0c00936 1535-3907 |
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metadata only access info:eu-repo/semantics/openAccess |
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metadata only access |
eu_rights_str_mv |
openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
ACS Publications |
publisher.none.fl_str_mv |
ACS Publications |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799130333800562688 |