Tapirira guianensis Aubl. Extracts inhibits proliferation and migration of oral cancer cells lines
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/45061 |
Resumo: | Cancer of the head and neck is a group of upper aerodigestive tract neoplasms in which aggressive treatments may cause harmful side effects to the patient. In the last decade, investigations on natural compounds have been particularly successful in the field of anticancer drug research. Our aim is to evaluate the antitumor effect of Tapirira guianensis Aubl. extracts on a panel of head and neck squamous cell carcinoma (HNSCC) cell lines. Analysis of secondary metabolites classes in fractions of T. guianensis was performed using Nuclear Magnetic Resonance (NMR). Mutagenicity effect was evaluated by Ames mutagenicity assay. The cytotoxic effect, and migration and invasion inhibition were measured. Additionally, the expression level of apoptosis-related molecules (PARP, Caspases 3, and Fas) and MMP-2 was detected using Western blot. Heterogeneous cytotoxicity response was observed for all fractions, which showed migration inhibition, reduced matrix degradation, and decreased cell invasion ability. Expression levels of MMP-2 decreased in all fractions, and particularly in the hexane fraction. Furthermore, overexpression of FAS and caspase-3, and increase of cleaved PARP indicates possible apoptosis extrinsic pathway activation. Antiproliferative activity of T. guianensis extract in HNSCC cells lines suggests the possibility of developing an anticancer agent or an additive with synergic activities associated with conventional anticancer therapy. |
id |
RCAP_117b9ac3ea00e3dc68277d8db6987b99 |
---|---|
oai_identifier_str |
oai:repositorium.sdum.uminho.pt:1822/45061 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Tapirira guianensis Aubl. Extracts inhibits proliferation and migration of oral cancer cells linesHNSCCCytotoxic activityAlkaloidsApoptosis markersScience & TechnologyCancer of the head and neck is a group of upper aerodigestive tract neoplasms in which aggressive treatments may cause harmful side effects to the patient. In the last decade, investigations on natural compounds have been particularly successful in the field of anticancer drug research. Our aim is to evaluate the antitumor effect of Tapirira guianensis Aubl. extracts on a panel of head and neck squamous cell carcinoma (HNSCC) cell lines. Analysis of secondary metabolites classes in fractions of T. guianensis was performed using Nuclear Magnetic Resonance (NMR). Mutagenicity effect was evaluated by Ames mutagenicity assay. The cytotoxic effect, and migration and invasion inhibition were measured. Additionally, the expression level of apoptosis-related molecules (PARP, Caspases 3, and Fas) and MMP-2 was detected using Western blot. Heterogeneous cytotoxicity response was observed for all fractions, which showed migration inhibition, reduced matrix degradation, and decreased cell invasion ability. Expression levels of MMP-2 decreased in all fractions, and particularly in the hexane fraction. Furthermore, overexpression of FAS and caspase-3, and increase of cleaved PARP indicates possible apoptosis extrinsic pathway activation. Antiproliferative activity of T. guianensis extract in HNSCC cells lines suggests the possibility of developing an anticancer agent or an additive with synergic activities associated with conventional anticancer therapy.The authors acknowledge CNPq, FAPEMIG (APQ-00953-12), Ministerio da Ciencia, Tecnologia e Inovacao-MCTI, and Fundo Nacional de Saude-FNS (1302/13), FINEP (MCTI/FINEP/MS/ SCTIE/DECIT-01/2013-FPXII-BIOPLAT) and CAPES (Brazilian agencies) for the financial support of this study. We thank Arali Aparecida Costa Araujo for her support in collecting of T. guianensis and Joao Maximo De Siqueira (UFSJ) and Paulo Cezar Vieira (UFSCar) for their support in the phytochemical analysis.info:eu-repo/semantics/publishedVersionMDPI AG[et. al.]Universidade do MinhoOliveira, Renato José SilvaLopes, Gabriela FrancineCamargos, Luiz FernandoRibeiro , Ana MacielSantos, Fábio Vieira dosSeverino, Richele PriscilaSeverino, Vanessa Gisele PasqualottoTerezan, Ana PaulaThomé, , Ralph GruppiReis, R. M.2016-11-142016-11-14T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/45061engSilva-Oliveira, R. J., Lopes, G. F., Camargos, L. F., Ribeiro, A. M., dos Santos, F. V., Severino, R. P., . . . Ribeiro, R. I. M. A. (2016). Tapirira guianensis Aubl. Extracts inhibit proliferation and migration of oral cancer cells lines. International Journal of Molecular Sciences, 17(11). doi: 10.3390/ijms171118391661-659610.3390/ijms1711183927834805http://www.mdpi.com/1422-0067/17/11/1839info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:37:55Zoai:repositorium.sdum.uminho.pt:1822/45061Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:34:16.539097Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Tapirira guianensis Aubl. Extracts inhibits proliferation and migration of oral cancer cells lines |
title |
Tapirira guianensis Aubl. Extracts inhibits proliferation and migration of oral cancer cells lines |
spellingShingle |
Tapirira guianensis Aubl. Extracts inhibits proliferation and migration of oral cancer cells lines Oliveira, Renato José Silva HNSCC Cytotoxic activity Alkaloids Apoptosis markers Science & Technology |
title_short |
Tapirira guianensis Aubl. Extracts inhibits proliferation and migration of oral cancer cells lines |
title_full |
Tapirira guianensis Aubl. Extracts inhibits proliferation and migration of oral cancer cells lines |
title_fullStr |
Tapirira guianensis Aubl. Extracts inhibits proliferation and migration of oral cancer cells lines |
title_full_unstemmed |
Tapirira guianensis Aubl. Extracts inhibits proliferation and migration of oral cancer cells lines |
title_sort |
Tapirira guianensis Aubl. Extracts inhibits proliferation and migration of oral cancer cells lines |
author |
Oliveira, Renato José Silva |
author_facet |
Oliveira, Renato José Silva Lopes, Gabriela Francine Camargos, Luiz Fernando Ribeiro , Ana Maciel Santos, Fábio Vieira dos Severino, Richele Priscila Severino, Vanessa Gisele Pasqualotto Terezan, Ana Paula Thomé, , Ralph Gruppi Reis, R. M. |
author_role |
author |
author2 |
Lopes, Gabriela Francine Camargos, Luiz Fernando Ribeiro , Ana Maciel Santos, Fábio Vieira dos Severino, Richele Priscila Severino, Vanessa Gisele Pasqualotto Terezan, Ana Paula Thomé, , Ralph Gruppi Reis, R. M. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
[et. al.] Universidade do Minho |
dc.contributor.author.fl_str_mv |
Oliveira, Renato José Silva Lopes, Gabriela Francine Camargos, Luiz Fernando Ribeiro , Ana Maciel Santos, Fábio Vieira dos Severino, Richele Priscila Severino, Vanessa Gisele Pasqualotto Terezan, Ana Paula Thomé, , Ralph Gruppi Reis, R. M. |
dc.subject.por.fl_str_mv |
HNSCC Cytotoxic activity Alkaloids Apoptosis markers Science & Technology |
topic |
HNSCC Cytotoxic activity Alkaloids Apoptosis markers Science & Technology |
description |
Cancer of the head and neck is a group of upper aerodigestive tract neoplasms in which aggressive treatments may cause harmful side effects to the patient. In the last decade, investigations on natural compounds have been particularly successful in the field of anticancer drug research. Our aim is to evaluate the antitumor effect of Tapirira guianensis Aubl. extracts on a panel of head and neck squamous cell carcinoma (HNSCC) cell lines. Analysis of secondary metabolites classes in fractions of T. guianensis was performed using Nuclear Magnetic Resonance (NMR). Mutagenicity effect was evaluated by Ames mutagenicity assay. The cytotoxic effect, and migration and invasion inhibition were measured. Additionally, the expression level of apoptosis-related molecules (PARP, Caspases 3, and Fas) and MMP-2 was detected using Western blot. Heterogeneous cytotoxicity response was observed for all fractions, which showed migration inhibition, reduced matrix degradation, and decreased cell invasion ability. Expression levels of MMP-2 decreased in all fractions, and particularly in the hexane fraction. Furthermore, overexpression of FAS and caspase-3, and increase of cleaved PARP indicates possible apoptosis extrinsic pathway activation. Antiproliferative activity of T. guianensis extract in HNSCC cells lines suggests the possibility of developing an anticancer agent or an additive with synergic activities associated with conventional anticancer therapy. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-11-14 2016-11-14T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/45061 |
url |
http://hdl.handle.net/1822/45061 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Silva-Oliveira, R. J., Lopes, G. F., Camargos, L. F., Ribeiro, A. M., dos Santos, F. V., Severino, R. P., . . . Ribeiro, R. I. M. A. (2016). Tapirira guianensis Aubl. Extracts inhibit proliferation and migration of oral cancer cells lines. International Journal of Molecular Sciences, 17(11). doi: 10.3390/ijms17111839 1661-6596 10.3390/ijms17111839 27834805 http://www.mdpi.com/1422-0067/17/11/1839 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI AG |
publisher.none.fl_str_mv |
MDPI AG |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799132864188514304 |